RT&T-AMP-MERFISH enables whole-transcriptome-scale, isoform-resolved spatial imaging of single cells in intact tissues and reveals how gene programs and transcript isoform usage vary across cell types...

A photolabile oligonucleotide system enables spatially resolved single-cell measurements to show how the epigenome regulates gene expression.

The actin-related protein 2/3 (Arp2/3) complex is a central nucleator of branched actin filaments, essential for numerous cellular processes. Using superresolution imaging, we demonstrate that Arp2/3 ...

A new study reveals that aging hematopoietic stem cells trigger a form of innate immune memory, known as trained immunity, that fuels the chronic low-grade inflammation that underlies age-related phys...

Epithelial lumen formation is fundamental to organ development, yet how apical surfaces first emerge remains unclear. Ghosh, Martin, and Chen et al., show that lumen initiation is driven via bulk deli...

Nature Reviews Molecular Cell Biology - Single-molecule genomics methods are used to study the activity of regulatory factors on individual DNA molecules genome-wide, thereby enabling...

The genomic regions regulating gene expression are often themselves transcribed into a variety of noncoding RNAs (ncRNAs). However, the regulatory roles of this noncoding transcription remain largely ...

This Review discusses how rare-disease-causing variants in the noncoding genome impact gene regulation, why these examples are so few and how new approaches could accelerate discovery of noncoding var...

Le et al. show that in Xenopus embryos, local ectodermal tissue flows provide a directional cue that polarizes and guides embryonic myeloid cells migration via cell-on-cell interactions.

Inflammation induces interchromosomal DNA interactions that bring parent genes into close proximity, facilitating the formation of chimeric mRNAs that encode physiologically relevant, functional prote...

More than twenty years ago, the FANTOM consortium uncovered that mammalian genomes are pervasively transcribed, revealing multitudes of RNAs with unknown functions. A subset of these transcripts has s...

Microtubules are essential components of the cytoskeleton, yet important links between their molecular properties and organismal functions remain unresolved. In this Roadmap, Janke et al. discuss key ...

Cell Press article: mRNA 3′ UTRs chaperone intrinsically disordered regions to control protein activity

Cellular processes often exhibit irreversible state transitions even after external signals responsible for a transition are withdrawn. Long-standi...

Here the authors show that, under stress, epithelial cells reveal an unexpected role for a chromatin factor in directing mRNA to cell protrusions, enabling local organelle assembly and cell movement, ...

This study introduces iChIP2, a low-input chromatin immunoprecipitation followed by sequencing method that profiles histone post-translational modifications (hPTMs) simultaneously across diverse eukar...

Mammals display prominent diversity in the ability to regenerate damaged ear pinna, but the genetic changes underlying the failure of regeneration remain elusive. We performed comparative single-cell ...

Integrin adhesions allow dynamic cell-matrix interactions to regulate cell behaviour. Here, the authors reveal that tensin 1, a multidomain adaptor protein, forms condensates in cells that act as reve...