To, Pett, Dufva et al. perform single-nucleus RNA and ATAC sequencing, as well as spatial transcriptomics, to study human meninges at 6–21 weeks post conception.

Meiring, Mahapatra et al. show that expanded microtubule lattice conformations, such as those induced by small-molecule taxanes, can lead to the release of GEF-H1 and thus induce RhoA activation.

Li, Jiang and colleagues present DePass, a graph-based deep learning framework for integrating paired multi-omics data in spatial and single-cell contexts. A dual-enhancement approach is used to remov...

Recent changes in Genentech’s scientific organization, occurring as the company makes artificial intelligence (AI) increasingly central to its research strategy, highlight a broader question facing bi...

Two studies show that the ER-associated protein CLN8 catalyses the acyl CoA-dependent acylation of glycerophosphoglycerol, producing lysophosphatidylglycerol, which is then converted into BMP. These r...

Radulovic et al. show that phosphatidylinositol 3-phosphate (PI3P), produced by the phosphatidylinositol 3-kinase PIK3C3, is formed on ER domains after lysosome damage. ER domain PI3P helps to recruit...

Xie, Tan, Mo and colleagues show that a liver-specific bipartite structure of the active X chromosome is lost early in hepatocarcinogenesis in mice and humans, driving X-linked gene activation and pre...

Xu, Guo et al. report suppressor screens in Caenorhabditis elegans to counteract tubulin mutants. They report several missense variants in tubulin genes that may restore microtubule architecture and d...

Biayna, Baranovskii and colleagues report that intron-retained RNAs derived from cell cycle- and genome maintenance-related genes are pooled at the periphery of nuclear speckles and undergo synchronou...

Le et al. show that in Xenopus embryos, local ectodermal tissue flows provide a directional cue that polarizes and guides embryonic myeloid cells migration via cell-on-cell interactions.

This Perspective highlights the use of current proteomics technologies in defining stage-specific reprogramming events to understand reproductive ageing, improve oocyte quality, and refine the outcome...

This Review discusses the spatial organization of nucleotide metabolism and its integration with cellular signalling pathways, emphasizing its importance as a dynamic regulatory axis in both physiolog...

Li, Jihad and colleagues develop an organoid co-culture system, alongside patient tissue and mouse model analyses, to model, characterize and compare cellular interactions between epithelia and stroma...

This Perspective discusses emerging evidence for non-cell-autonomous propagation of ferroptotic cell death and highlights the mechanisms that underlie its restriction in tissues, as well as ferroptosi...

The detailed study of membrane contact sites is challenging owing to their small size and highly dynamic nature. This Review highlights advanced imaging methods and available biosensors that can be us...

Gao et al. show that yeast grow faster by proportionally increasing mRNA and ribosome concentrations to increase actively translating ribosomes, while elongation remains constant. A mass-action model ...

Cavanaugh et al. show that embryos from aged mice have increased contractility and tissue viscosity on embryonic day 4.5, which leads to poor spreading and attachment. Similar age-associated mechanica...

Chen, Zhang and colleagues report ctEV-CLOCK as a tool to characterize circulating tumour-derived extracellular vesicles and demonstrate a pattern of circadian oscillation, which may be leveraged to d...

Peng, Xue et al. develop a nucleic acid metabolites detection method, DNA RNA Elements Areal Mass Spectrometry (DREAMS) which enables the identification of DNA and RNA modifications, as well as spatia...

Lebrec et al. present a FRET-based cGAS reporter and show that cGAS activation by genotoxic stress is rare and uncoupled from micronuclei. The study implicates R-loop structures and subtypes of chroma...

Kim et al. demonstrate a non-canonical function for EZH2 in modulating cholesterol metabolism and tumour development by interacting with SREBP2, which may be targeted by PROTACs to disrupt its oncogen...

Advances in electron microscopy instrumentation and methods continue to drive progress in the physical and life sciences. The 4th edition of this conference will bring together researchers from physic...

Zhao et al. describe an enhancer-dense nuclear microenvironment in the cerebellum that includes long neuronal genes and mega-enhancers. This microenvironment partly depends on the transcription factor...

Li et al. report that FLT3 inhibition impairs selenoprotein translation and induces ferroptosis to suppress FLT3-mutant leukaemia. This can be counteracted by dietary vitamin E, which may disrupt the ...

Chen, Xu, Zhou, Wu et al. report that a citric acid cycle enzyme OGDH regulates METTL3 succinylation and its activation as an m6A reader, thereby modulating NRF2 translation, disulfidptosis evasion an...

Aygenli et al. map chromatin-bound CLOCK/BMAL1 protein complexes across three mouse tissues and uncover PROX1, HNF1B and HOXA5 as homeodomain cofactors that directly bind and co-occupy BMAL1 sites to ...

Mistry et al. propose that Dnmt3a-mutant haematopoietic cells induce mesenchymal stromal cell senescence. Removal of senescent non-haematopoietic cells reduces Dnmt3a-mutant haematopoiesis.

Brandon, Robinson-Thiewes, Kaulage et al. report that targeted BRD4 recruitment enables transcription through H3K9me3/HP1-marked heterochromatin without erasing repressive marks, identifying condensat...

Zhang, Zhao, Liang et al. report that, independent of its catalytic function, the glycolytic enzyme PGAM1 acts as a scaffold protein to regulate autophagy activation, thereby modulating cellular homeo...

Henneman et al. report an unexpected role for class 3 PI3K (PI3K-3) acting as a transcriptional co-activator during fasting. They show that nuclear PI3K-3 co-activates the methyltransferase Set1/COMPA...

Using a dynamic fluorescent reporter, Tang et al. demonstrate that cancer stem cells are hyper-responsive to microenvironmental cues, which they sense and adapt to through YAP/TAZ signalling, thereby ...

Johnson et al. combine single-cell and spatial transcriptomics approaches to build a spatially resolved atlas of the developing human intestine and intestinal organoids, uncovering spatial organizatio...

Luo et al. describe a budding type of lysosome fission linked to delivery of MFF from mitochondrial-derived vesicles to lysosomes. MFF recruits DRP1 for membrane scission. This axis restores lysosome ...

Yao, Li, Gai et al. profile normal coronary artery and early and advanced coronary atherosclerosis samples from humans using single-cell transcriptomics and identify a progenitor-like endothelial subt...

Yao, Li, Gai et al. profile normal coronary artery and early and advanced coronary atherosclerosis samples from humans using single-cell transcriptomics and identify a progenitor-like endothelial subt...

Zhou, Wang et al. dissect the role of PRC2 in early embryogenesis through inducible degradation of EED, a core PRC2 subunit, and report that PRC2 is required for proper maternal-to-zygotic transition ...

Jha et al. show that the transmembrane protein CD44 restricts EGFR mobility to maintain an EGFR–PI3K–Rac gradient during bleb-based cell migration.

Paraschiakos, Yuan et al., using a computational pipeline, identify the short linear actin filament-binding motif (SFM) as an evolutionarily conserved actin filament-binding motif present in 103 human...

Nishimura et al. develop a platform named cell surface liposome binding (CLiB), which allows the high-throughput monitoring of protein–lipid interactions and can be used to design lipid-binding probes...

Liu, Huang, Shen, Shao, Jing and collaborators combine single-cell transcriptomics with Patch-seq and metabolomics to dissect primate anterior insular cortex. They identify two von Economo neuron (VEN...

Liu, Huang, Shen, Shao, Jing and collaborators combine single-cell transcriptomics with Patch-seq and metabolomics to dissect primate anterior insular cortex. They identify two von Economo neuron (VEN...

Xie et al. demonstrate that extrachromosomal DNA tethering to mitotic chromosomes is in part mediated by H3K27ac-marked chromatin, associated chromatin binders and transcription-associated cofactors.

Bae et al. identify MED4 as a regulator of tumour dormancy from a genome-wide CRISPR screen, and show that loss of MED4 alters the enhancer landscape and induces extracellular matrix remodelling to dr...

Nguyen et al. show that accumulation of dimethylarginine in lysosomes in disease models is associated with lipotoxicity and lipid droplet abundance.

Diao, Zou et al. demonstrate that activation of the integrated stress response drives lineage dedifferentiation and tumour progression in lung adenocarcinoma, associated with aberrant mitochondrial fu...

Because of their ability to simulate certain aspects of embryonic development, stem cell-based embryo models can be a promising tool in our efforts to better ...