A spatial lipidomic atlas of the mouse brain reveals more than 500 biochemical territories that mirror cell types and connectivity, capture oligodendrocyte heterogeneity and ventricular zonation, and ...

Mouse genetics and computer modeling suggest that a reaction-diffusion mechanism defines digit pattern.

Prime assembly enables RNA-guided integration of medium-to-large DNA sequences in human cells without requiring double-strand breaks or cell cycle progression, and supports exon recoding, transgene in...

This study shows that the brain arises from two distinct progenitors: one forms the forebrain and midbrain, whereas the other generates the hindbrain. These insights enable the generation of human hin...

Xenocortication with human neurons enables circuit- and behaviour-level analysis of neurodevelopment in mice.

Scaffolded DNA Computer performs diverse computations while thermodynamically relaxing towards equilibrium, demonstrating simplicity, reusability, speed, robustness and scalability of the approac...

The genomic regions regulating gene expression are often themselves transcribed into a variety of noncoding RNAs (ncRNAs). However, the regulatory roles of this noncoding transcription remain largely ...

An imaging system developed to record cellular activity throughout the whole body of zebrafish captures cellular organ dynamics and identifies multiple distributed circuits.

CFH is a key mediator of neuronal resilience across the central nervous system in mice and humans.

In senescent cells, mitochondria-derived acetyl-CoA promotes histone acetylation and increases chromatin accessibility at inflammatory gene loci. Inhibition of SLC25A1 attenuates these effects, unders...

Whether inflammation leaves a lasting imprint on the intestinal stem cell (ISC) niche has remained unknown. Adir et al. find that persistent modified ECM (modECM) encodes inflammatory memory, reshapes...

Human brain organoids were developed for over five years in culture to demonstrate that brain cells can continue to mature and record the passage of time, following human-specific endogenous programs,...

Amygdala astrocyte primary cilia are disrupted during stress, and their restoration leads to improvements in stress-related behaviour in mice.

GOOSE enables the design and testing of thousands of disordered protein region sequences to reveal distinct sequence-to-function relationships.

Changes in gene expression have been observed in the aging human brain, but our understanding of the underlying regulatory mechanisms remains limited. To unravel these complexities, we analyzed single...

Nature - Somatic mutations reveal the ontogeny of microglia in human aging

Kilobase-scale nickase-targeting (KNIT) editing enables single-nick-based DNA insertion across genomic loci and cell types, supporting insertion of DNA fragments exceeding 10 kb, with high effici...

In senescent cells, mitochondria-derived acetyl-CoA promotes histone acetylation and increases chromatin accessibility at inflammatory gene loci. Inhibition of SLC25A1 attenuates these effects, unders...

Alternate RNA decoding, an understudied process, leads to peptide sequence modifications that can have substantial functional effects on protein stability, tissue-specific proteomes and dise...

Mammals display prominent diversity in the ability to regenerate damaged ear pinna, but the genetic changes underlying the failure of regeneration remain elusive. We performed comparative single-cell ...

Aging disrupts tissue homeostasis across organ systems. Here, we identify tissue-resident macrophages (TRMs) as central coordinators of age-related organ decline through impaired clearance of senescen...

High-resolution spatial transcriptome analysis of a human embryo at Carnegie stage 6 reveals three distinct developmental trajectories from the epiblast towards amnion, primitive streak and axial meso...

The cerebellum ages unevenly, with some regions showing preservation that may help protect the brain from decline. The authors show that large cerebellar volume is associated with stronger cognitive r...

Semaglutide attenuates brain inflammation in mice, coordinately reversing inflammatory gene programs across diverse brain cell types likely engaging both neuronal circuits and peripheral immune respon...

Microglia-mediated neuroinflammation drives Alzheimer’s disease progression. In this article, Liu et al. show that the histone acetyltransferase KAT7 promotes mitochondrial DNA-dependent inflammation ...

Tosoni and colleagues identify immature neurons in the aged human hippocampus with signatures of younger biological age and cellular fitness. Their molecular programs and intercellular interactions ar...

Recent memories are consolidated during sleep by spontaneous reactivation. However, whether and how memory reactivation affects sleep dynamics remain unclear. By tracking and modulating memory activit...

Microglia cellular changes underlie divergent resilience-associated mechanisms in human aging.

The DishBrain system is the first real-time synthetic biological intelligence platform that demonstrates that biological neurons can adjust firing activity in a way that suggests the ability to learn ...

AhR functions as a neuronal brake on axon regeneration, integrating environmental sensing, protein homeostasis and metabolic signalling to control the balance between stress adaptation and axonal repa...

Integration of gene expression data from multiple tissues across four mammalian species reveals conserved transcriptomic signatures of mammalian ageing and mortality and uncovers the modular...

Using xenograft inflammation–recovery models and single-cell multiomics, a haematopoietic stem cell population after inflammatory stress is identified.

Single-cell transcriptomics is revolutionizing our understanding of cellular diversity, yet comparing transcriptional programs across the tree of life remains challenging. We developed TranscriptForme...

In PS19 mice, microglia-derived glycoprotein nonmetastatic melanoma protein B promotes mitochondrial extracellular vesicle secretion, enabling mitochondrial transfer to astrocytes. This improves astro...

Decima learns to predict cell-type-specific gene expression based on DNA sequences, contributing to the analysis of gene regulation and design of context-specific regulatory elements.

Persons carrying loss-of-function variants of proprotein convertase subtilisin–kexin type 9 (PCSK9) have reduced levels of low-density lipoprotein (LDL) cholesterol and fewer atherosclerotic cardio...

Nature - An AI system to help scientists write expert-level empirical software

Systems-level analyses of organ intrinsic nervous systems reveal that these networks are initially configured by lineage-dependent programmes, and their architecture and molecular identity are refined...

Myeloid cells show marked heterogeneity in Alzheimer’s disease. This study introduces CODEX-CNS, a single-cell spatial proteomics pipeline, and identifies a human microglial subpopulation enriched in ...

A mitochondrial transplantation approach rescues mitochondrial deficiency and prevents mitochondrial DNA depletion syndrome, Leigh syndrome, and Parkinson’s disease in cellular and mouse models.

Old T cells secrete an enzyme that can impair brain function in mice.

INSTALL overcomes fundamental challenges for DNA delivery and integration methods by synergizing immune-stealth nucleic acids with recombinases to enable kilobase-scale integration strategies without ...

Systems-level analyses of organ intrinsic nervous systems reveal that these networks are initially configured by lineage-dependent programmes, and their architecture and molecular identity are refined...

Connexin proteins found in white perch fish were used to engineer synthetic electrical synapses, enabling precision circuit editing in mammals.

Quenching mitochondrial reactive oxygen species in monocyte-derived macrophages, but not in microglia, ameliorates neuroinflammation.