Abstract. Efficient purification of specific RNAs from lysates remains a major challenge. Existing methods, such as MS2 tagging or oligonucleotide hybridiz

Cryogenic electron microscopy captures nucleotide excision repair complexes assembling on damaged DNA templates.

Innate immune recognition of bacteria is thought to center on cell-surface or endosomal pattern recognition receptors such as TLRs. Ragland et al. identify an intraphagosomal cyclic dinucleotide (CDN)...

The design of RNA-guided nucleases with properties not limited by evolution can expand programmable genome-editing capabilities. However, generating diverse multidomain proteins with robust enzymatic ...

LEAPER 3.0, a structurally optimized dual-bulge ADAR-recruiting RNA design platform, achieves precise and efficient endogenous A-to-I RNA editing across diverse ADAR contexts.

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Yang et al. reveal a dynamic DdmDE-mediated plasmid clearance pathway. Transient DNA bubbles permit promiscuous DdmE binding, while target specificity arises from slower dissociation at matched sites. DdmE then recruits a DdmD dimer to drive target-centered, bidirectional dsDNA unwinding. Free DdmD subsequently coats extruded ssDNA to enable site-specific endonucleolytic cleavage.

Biochemical, structural and cell biological analyses reveal that UAP56 (DDX39B) assembles with a TREX-2–like module that redirects non-functional polyadenylated RNAs from export to degradati...

NSUN2 is an enzyme that adds a small chemical mark to RNA molecules and uniquely selects its targets based on specific sequence patterns and structural features.

Abstract. Apurinic/apyrimidinic (AP) sites are among the most frequent DNA lesions, arising thousands of times per cell each day. These lesions threaten ge

Singh et al. combine cryo-electron microscopy and functional studies to reveal how a single protein complex selects diverse mRNAs for subcellular localization using a combination of shape, positional ...

ADP-ribosylation regulates DNA repair, but its distinct forms have been difficult to distinguish. Here, the authors develop sensitive, modular antibodies to precisely detect mono- and poly-ADP-ribosyl...

Some scientists are too eager to believe their own claims

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How ribosome-bound NAC distinguishes mitochondrial precursors from ER clients has remained unclear. Here, authors reveal a molecular switch in NAC that limits SRP access to nascent mitochondrial precu...

NAC enables cotranslational N-terminal processing of histones H2A and H4 by recruiting MetAP1 and NatD at the ribosomal tunnel.

During eukaryotic transcription, Pol II must overcome nucleosome obstacles and, because of DNA’s helical structure, must also rotate relative to DNA, generating torsional stress. However, there is lim...

Accumulating evidence has connected the chemically unstable, DNA-damaging gut bacterial natural product colibactin to colorectal cancer, including the identification of mutational signatures that are ...

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Natural genetic transformation is a conserved mechanism of bacterial horizontal gene transfer, which is directed entirely by the recipient cell and...

The kinase ZAK is activated at collided ribosomes to mediate the ribotoxic stress response.

Reconstituting 20-kb chromatin shows that tuning acetylation alone reshapes its folding, dynamics, and contact domain formation.