Abstract. Pre-mRNA splicing is an essential step in eukaryotic gene expression during which spliceosomes remove introns from nascent RNAs while ligating th

The control of gene expression is central to cellular physiology and to various biotechnological applications. In higher eukaryotes, synthesis of most messenger RNAs involves removal of introns during...

Trypanosomatid parasites rely on Spliced Leader (SL) RNA trans-splicing to produce every mature mRNA. Here, the authors present cryo-EM structures of the trans spliceosome during SL exon ligation, pro...

A small, spotted tiger cat from Bolivia may just be the beginning of a wave of new small cat species.

Using mass photometry combined with a single-molecule trapping method allows real-time monitoring of the assembly of individual virus-like particles with molecular resolution, enabling observation and...

View Projected Impact of the FY2027 Budget Cuts to the NIH and NSF

The Dollywood Foundation, home of Dolly Parton’s Imagination Library, today announced Give Like Dolly Day on September 25, a global day dedicated to supporting local Dolly Parton’s Imagination Library...

The Physics of Life at the Nanoscale Conference, 2027

ALT: Ribonucleic Acid Rna

Pre-mRNA secondary structure can modulate the regulatory function of intronic sequences by masking or exposing splice-site signals and altering the accessibility of other features. Yet experimentally ...

The Physics of Life at the Nanoscale Conference, 2027

Much pre-mRNA splicing initiates co-transcriptionally, but its mechanism is unclear. We investigated this process by degron depletion of the U1-70K subunit of U1 snRNP that contacts transcribing RNA p...

Cryo-EM and genetic analyses reveal that the SWI/SNF chromatin remodeler is an integral component of the transcription pre-initiation complex. The structures demonstrate how SWI/SNF facilitates transcription initiation through stabilization of PIC organization and concerted remodeling of the +1 nucleosome.

Abstract. Fluorescence lifetime (FL) is a robust strategy for multiplexed imaging; however, variability in fluorophores’ local microenvironment when used o

Understanding how the spliceosome integrates regulatory cues to generate RNA diversity remains a central question in gene expression control. Emerging evidence reveals a multilayered framework in whic...

Bacterial ribosomes can synthesize proteins encoded within their own RNA.

Arp2, a subunit within the actin nucleator Arp2/3, encodes two splice variants that differ by five residues in the D-loop, a critical element for catalyzing actin polymerization. It is unknown if this...

The Physics of Life at the Nanoscale Conference, 2027

Nuclear speckles are prominent biomolecular condensates enriched in RNA-processing factors. Although historically viewed as reservoirs for spliceosoma…

TDP-43 is an essential RNA-binding protein. Here, the authors show how it binds single-stranded poly(UG) RNA through high affinity 1D lattice interactions. The N-terminal domain functions to accelerat...

The hyphae-to-yeast morphological transition promotes dissemination of the pathogen Candida albicans, but the mechanisms underlying this process are poorly defined. By identifying a bacterial metaboli...

We study how host alternative splicing shapes the outcome of influenza virus infection — from cryptic isoforms that antagonize the polymerase to the ANP32 proteins that support it.