Professor Alexei Maklakov, School of Biological Sciences, University of East Anglia – contact me Professor Tracey Chapman, UEA/BIO Professor Simone Immler, UEA/BIO Why do some animals continue to live...

The emergence of reproductive barriers between closely related populations is a fundamental step in speciation, yet the underlying genetic mechanisms remain poorly understood in mammals. Here we resol...

Abstract. Paramutation is an interaction of alleles resulting in heritable gene silencing, but the molecular events that initiate paramutation are largely

In Drosophila, the protein Lsp2, which is induced by dietary essential amino acids, is a key physiological effector of mTORC1, and mutant flies that lack the Lsp2 gene exhibit reduced translation of T...

In Drosophila, the protein Lsp2, which is induced by dietary essential amino acids, is a key physiological effector of mTORC1, and mutant flies that lack the Lsp2 gene exhibit reduced translation of T...

A comparative analysis of multiomics data from embryonic and adult tissues of Atlantic salmon and rainbow trout reveals developmental and tissue-specific constraints shaping regulatory evolution follo...

Cell viability depends on the precise expression of essential genes, which are controlled by CpG-island (CGI) promoters densely bound by transcription factors (TFs). This has led to the prevailing model that TFs cooperate to ensure ubiquitous expression. Here, using rapid and reversible single and combinatorial degradation in murine stem cells, we systematically dissect the regulatory interactions between five key TFs. We uncover an unexpectedly specific architecture in which regulatory dominance, rather than cooperation, is the prevailing mode, where individual TFs autonomously drive chromatin opening and gene activation at largely distinct promoters. Cooperative regulation occurs at a minority of sites with antagonistic or synergistic outcomes modulated by the interplay between nucleosome positioning and TF sensitivity to chromatin. This logic is recapitulated at synthetic sequences and reflected in human genetic variation. These findings reveal that single TFs dominantly activate distinct sets of CGI-linked genes, including essential genes, across development, homeostasis, and disease. ### Competing Interest Statement The authors have declared no competing interest. DFG, GR 6341/2-1, 556634773

Abstract. The evolution of de novo genes from ancestrally nontranscribed sequences has been reported in a wide range of taxa. However, the DNA sequence cha

Schargel et al. engineer TnpB-family proteins into minimal de novo RNA-guided transposition systems. Nuclease-deficient TnpB-family proteins enable three-component DNA integration with high efficiency, target-site immunity, orientation control, and programmable insertion into the bacterial chromosome. In parallel, mutations to the TniQ-TnsC interface in wild-type V-K CAST attenuate off-site transposition.

Ribosome hibernation helps cells survive stress by reversibly silencing translation and preserving ribosomal complexes. Although well characterized in bacteria and eukaryotes, archaeal hibernation rem...

Protein synthesis declines with age across many tissues, but whether this decline actively contributes to physiological aging remains unclear. Oocytes may be particularly vulnerable because they rely ...

The molecular factors and mechanisms that mediate the chromatin recruitment of activation-induced deaminase and the generation of its stalled polymerase II substrate during somatic hypermutation are i...

Abstract. Translation initiation involves a concerted set of intermolecular interactions that efficiently recognize optimal AUG start codons. However, tran

Alternative RNA processing generates extensive transcript diversity, yet how transcript architecture influences selective m6A deposition is incompletely understood. Exon-junction-based models explain ...

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RNA modifications play an important role in biological processes. Mapping the diversity of RNA chemistry and studying the biological function of in...

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Single-cell transcriptomics, spatial transcriptomics and chromatin accessibility maps of the human craniofacial region in developing embryos provide insights into the mechanisms underlying facial vari...

In eukaryotes, ribosome biogenesis initiates within the nucleolus, a hallmark multilayered compartment of the nucleus. Structures of pre-ribosomes have been characterized ex situ, but their assembly p...

Simulations integrating micro-C and imaging data provide a coherent view of chromatin organization from nucleosomes to clutches to domains, revealing that most regions form compact domains but short r...

Transposable elements (TEs) are key contributors to genome evolution, yet their macroevolutionary roles in adaptive radiations remain poorly understood, as high-quality genomic resources at sufficient...

Cyclin-dependent kinase 9 (CDK9) inhibition represents a promising therapeutic strategy in cancers that disrupts RNA polymerase II pause release and collapses oncogenic transcripts. Here, we show that...

Transposable elements mobilize within and occasionally between genomes, including from host to virus. We identified two insertions of the human BC200 noncoding RNA gene in the poxvirus molluscum conta...

Abstract. Efficient purification of specific RNAs from lysates remains a major challenge. Existing methods, such as MS2 tagging or oligonucleotide hybridiz

Plasmids drive bacterial adaptation, but how their diversity arises has remained unclear. Here, they show that in Staphylococcus aureus, mobile genetic elements repeatedly fuse and delete plasmids, re...

CRISPR-Cas provides RNA-mediated adaptive immunity, but how its first RNA-guided effector arose is unclear. In this study, we report the discovery of Viral Interference Programmable Repeat (VIPR) syst...

In the last decade, there has been an explosion in the development of in vivo germline genetic editing technologies in arthropods, first pioneered by the Receptor-Mediated Ovary Transduction of Cargo ...

Here, we combined ribosome profiling under conditions of mTOR inhibition and m⁶A mapping to identify distinct subsets of mRNAs that differ in their sensitivity to the suppression of cap-dependent initiation.

Here we utilize molecular dynamics simulations of FTO-RNA cap complexes to identify conserved aromatic residues on the surface of FTO involved in 5' cap recognition.

Biayna, Baranovskii and colleagues report that intron-retained RNAs derived from cell cycle- and genome maintenance-related genes are pooled at the periphery of nuclear speckles and undergo synchronou...

Rhynchospora tenuis undergoes obligate, genome-wide achiasmy in both male and female meiosis.

Development depends on gene regulation by enhancers across long genomic distances. This Perspective discusses mechanisms enabling long-range enhancer–promoter communication and the potential advantage...