iiinova — where your digital self keeps evolving

Reposted by Diorge Souza

Gabriel Oka @okagu.bsky.social · 28/08/2026

🧬 Happy to share our new preprint: ppigFinder, a desktop tool integrating bacterial genome analysis with large-scale AlphaFold 3 protein–protein interaction screening. We showcase it by exploring the VirD4 interactome of Xanthomonas citri. 💻 github.com/leepusp/ppig...

github.com

GitHub - leepusp/ppigfinder

Contribute to leepusp/ppigfinder development by creating an account on GitHub.

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Reposted by Diorge Souza

cryoEM papers @cryoempapers.bsky.social · 27/08/2026

ppigFinder: an integrated desktop application for bacterial genome annotation and AlphaFold 3 based protein protein interaction screening www.biorxiv.org/content/10.64898/2026.08.23.746524v1 #cryoEM

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Diorge Souza @diorgeps.bsky.social · 12/05/2026

How do bacteria avoid killing their own siblings while using contact-dependent weapons? In this work, a conserved DUF4189 protein (Xac2611/Tpf) blocks X-T4SS mediated killing, likely via interaction with VirB5. Great work led by my friend @okagu.bsky.social at the @chuckfarah.bsky.social lab

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Reposted by Diorge Souza

cryoEM papers @cryoempapers.bsky.social · 28/02/2026

A conserved archaeal ribosome-associated factor linking bacterial hibernation and eukaryotic energy sensing pubmed.ncbi.nlm.nih.gov/41757010/ #cryoEM

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Reposted by Diorge Souza

Alex Bisson @archaeon-alex.bsky.social · 24/02/2026

Another paper on a topic we never worked on before: a new ribosomal hibernation! Under the leadership of the super-postdoc Diorge, Davis Lab (ribo-cryoEM master), and @vikramalva.bsky.social (protein-evo whisperer), we identified AHA (not the song, but archaeal hibernation AMPKγ factor). (1/2)

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Diorge Souza @diorgeps.bsky.social · 24/02/2026

A fantastic position! The dream place to do science! 🤩

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Reposted by Diorge Souza

Vikram Alva @vikramalva.bsky.social · 23/02/2026

Very cool work led by @diorgeps.bsky.social from the labs of @archaeon-alex.bsky.social & Joey Davis (MIT)! They solved the Haloferax volcanii ribosome and characterize AHA, a conserved archaeal hibernation factor. With several recent related preprints, it’s an exciting moment for the field!

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Diorge Souza @diorgeps.bsky.social · 23/02/2026

A new preprint from the @archaeon-alex.bsky.social and Joey Davis (MIT) labs! We solved the structure of the ribosome from the archaeon Haloferax volcanii and discovered a new highly conserved ribosome hibernation factor that we named AHA (AMPKγ–HPF from Archaea) 🧵⬇️ www.biorxiv.org/content/10.6...

biorxiv.org

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Diorge Souza @diorgeps.bsky.social · 01/12/2025

So happy to see this come together! Huge congratulations to my friend @javierespadas.bsky.social and everyone involved. Amazing job!

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Reposted by Diorge Souza

Buzz Baum @buzzbaum.bsky.social · 01/12/2025

Javier has done beautiful work (following on from the study he did with Diorge Souza), showing how Asgard Escrt-III likely cut membranes. The answer: with Hofund.

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Reposted by Diorge Souza

Roux Lab, Geneva @rouxlab.bsky.social · 01/12/2025

Fantastic work from Javier @javierespadas.bsky.social in collaboration with @buzzbaum.bsky.social and @kaksonen.bsky.social labs, thank you Chris Toret, thank you Diorge @diorgeps.bsky.social!

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Reposted by Diorge Souza

Javier Espadas @javierespadas.bsky.social · 01/12/2025

New preprint from the lab!!🎉 We show that Asgard archaea ESCRT-III proteins can trigger membrane fission and reveal its molecular mechanism, offering clues to how these cells may have built internal compartments. But do these organisms even have these compartments? www.biorxiv.org/content/10.1...

biorxiv.org

Molecular basis for cellular compartmentalization by an ancient membrane fission mechanism

The emergence of cell compartmentalization depends on membrane fission to create the endomembrane compartments. In eukaryotes, membrane fission is commonly executed by ESCRT-III, a protein complex con...

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Reposted by Diorge Souza

Javier Espadas @javierespadas.bsky.social · 11/02/2025

Extremely happy to see our work finally out!🎉 Great collaboration between @rouxlab.bsky.social @buzzbaum.bsky.social labs and a real pleasure to work and share co-first authorship with incredible scientists and friends @diorgeps.bsky.social @sami-c.bsky.social. Big thanks to the rest of the authors!

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Reposted by Diorge Souza

MRC Laboratory of Molecular Biology @mrclmb.ac.uk · 11/02/2025

New research on ancient Asgard archaeal ESCRT-III proteins reveals evolutionary secrets of membrane remodelling. Diorge Souza, Javier Espadas and Sami Chaaban, investigated the ESCRT-III proteins with Buzz Baum and Aurelien Roux. Read more: www2.mrc-lmb.cam.ac.uk/asgard-archa... #LMBResearch (1/2)

Evolution, structure and membrane remodelling function of the ESCRT-III superfamily in eukaryotes and their closest relatives Asgard archaea. The two subfamilies, B-type and A-type, have distinct structural properties to perform sequential steps of the membrane remodelling pathway.

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Reposted by Diorge Souza

Roux Lab, Geneva @rouxlab.bsky.social · 10/02/2025

ESCRT-III is an ancient complex, present in Archaea, with only two proteins and all membrane remodelling functions. Great work by @javierespadas.bsky.social, @diorgeps.bsky.social, @sami-c.bsky.social, thanks to the @buzzbaum.bsky.social lab! Drawing by Julie Polge. www.science.org/doi/10.1126/...

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Diorge Souza @diorgeps.bsky.social · 08/02/2025

Happy to share our paper’s final version on the evolution, structure, and membrane binding / remodeling of Asgard ESCRT-III proteins! And my first 🦋 post! www.science.org/doi/10.1126/...

science.org

Asgard archaea reveal the conserved principles of ESCRT-III membrane remodeling

Asgard archaea have two ESCRT-III subunits with the conserved traits that drive stepwise membrane remodeling in eukaryotes.

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