Axonal localization of importin β1 is required for presynaptic functions that support spatial memory tasks.

Hitch-hiking of transcripts on organelles, including mitochondria, has emerged as a common pathway to transport mRNAs into the axon to enable their local translation. However, the extent of mitochondr...

Nature Neuroscience - Distinct neuron subtypes compartmentalize specific functions by differentially localizing and translating specific RNAs. This work identifies dynamic subtype- and...

Live-cell imaging of mRNA encoding secretome proteins and translated nascent peptide markers show that secretome translation occurs at endoplasmic reticulum junctions near lysosomes, requires lun...

Neurons rely on spatial and temporal control of protein synthesis to respond rapidly and locally to external stimuli, a process facilitated by the ...

How mitochondria positioning dynamically adapts to changes in cell shape is poorly understood. Here, Bradbury et al. show that cell-size-dependent trafficking of TRAK2 mRNA underpins the cell-size-sca...

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Translational repression by FMRP has been attributed primarily to ribosome stalling. We show that FMRP sequesters its target mRNAs from translation initiation. FMRP binds not only to the transcript body but also directly to cap-bound eIF4E and poly(A)-tail-bound PABPC1. Through binding these two proteins, FMRP protects its target mRNAs from translation and decay.

Spatiotemporal control of biomolecules is a key challenge in cell biology. Here, authors introduce RELISR, an optogenetic condensate system enabling reversible, light-dependent sequestration and relea...

Gene-targeting molecules known as antisense oligonucleotides help improve cognitive function in mouse models of a severe chromosomal disorder.

Activated Slack potassium channels bind translation regulators to stimulate translation of cortical β-actin mRNA.

CRISPR-TO, a system for programmable control of spatial localization of cellular RNAs, is presented and enables functional investigation of endogenous RNA localization in diverse living cells.

Subcellular RNA organization can affect critical cellular functions. However, our understanding of RNA microenvironments, particularly biomolecular condensates, remains limited, largely due to a lack ...

Synaptic neurotransmission is a critical hallmark of brain activity and one of the first processes to be affected in neural diseases. Monitoring this process, and in particular synaptic vesicle recycl...

Superfluous axons are removed from muscle fibers through temporal changes in mRNA translation.

Spatial RNA organization plays a pivotal role in diverse cellular processes and diseases, but the functional implications of spatial RNA localization remain underexplored. We present CRISPR-mediated t...

Researchers developed DELTA, a method for brain-wide measurement of synaptic protein turnover with single-synapse resolution, providing a powerful tool to localize and study mechanisms underlying syna...

Mitochondrial endosymbiosis was a pivotal event in eukaryotic evolution, requiring core proteins to adapt to function both within the mitochondria and in the host cell. Here, we systematically profile...

Nixon-Abell et al. show that ANXA11 condensation on lysosomal membranes causes a coupled phase transition of the underlying lipids and mechanical stiffening of the overall ensemble involved in RNP gra...

Axonal transcriptomes have been characterized, but their regulation and role in neuronal circuits remain unclear. Here authors uncover activity-dependent RNA localization in axons important for memory...

Cheng et al. identify a mitochondrial complex IV (CIV) deficiency in the brains of patients with sporadic amyotrophic lateral sclerosis (ALS). They demonstrate that defects in mitochondrial CIV induce...

Brain function requires precise spatiotemporal regulation of the neuronal proteome. To allow adaptation of the proteome in distal outposts of neurons,…

Depletion or inhibition of core stress granule proteins, G3BP1 in mammals and TIAR-2 in Caenorhabditis elegans, increases the growth of spontaneous...

Millions of ribosomes are packed within mammalian cells, yet we lack tools to visualize them in toto and characterize their subcellular composition. In this study, we present ribosome expansion micros...

Individual neurons have one or more axons that often extend long distances and traverse multiple microenvironments. However, it is not known how the composition of individual axons is established or l...

It is unclear how new lysosomal proteins are properly delivered in polarized neurons. Here, the authors developed a method combining the synchronization of protein release from the endoplasmic reticul...

Nature Biotechnology - Single-molecule live-cell fluorescence in situ hybridization uses an RNA-targeting CRISPR–Csm complex to image and track endogenous RNAs.