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Felipe Opazo

@opazo.bsky.social
1.4K followers 2.3K following 39 posts

PI at the Uni Medical Center Göttingen (UMG), Founder and CTO of NanoTag Biotechnologies. Using nanobodies for super-resolution microscopy in neurosciences. Opinions are my own.

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Reposted by Felipe Opazo
NanoTag Biotechnologies @nanotag.bsky.social · 06/02/2026
📄This new Journal of Microscopy 🔬 paper uses rigorous single-molecule assays to benchmark secondary nanobodies and shows NanoTag #SmartSecondaries® deliver high-affinity binding, efficient saturation, and exceptionally low off-target labeling for robust #multiplexed immunoassays 💪
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NanoTag Biotechnologies @nanotag.bsky.social · 17/04/2026
Never worked with #SmartSecondaries® (secondary nanobodies) for detecting conventional or recombinant primary antibodies? Here you can find more information: nano-tag.com/technology/s...
nano-tag.com
Smart Secondaries® - NanoTag Biotechnologies
Ideal secondary Ab replacement for One Step IF, higher resolution & species-independent multiplexing. Discover benefits of Smart Secondaries®!
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NanoTag Biotechnologies @nanotag.bsky.social · 28/09/2026
Spontaneous blinking. OneStep IF. Precise #SMLM. 🙌 Our Smart Secondaries® JF635b combine a self-blinking dye with small secondary #nanobodies for reduced linkage error, no clustering & fast OneStep IF. For #dSTORM & #MINFLUX 🔬✨ 🦙 Browse our JF635b-conjugated nanobodies here: bit.ly/4ffIkdB
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Mark Peifer (He, him) @peiferlabunc.bsky.social · 28/09/2026
Are you looking for a postdoc exploring the cell biology of development, defining how cells change shape and move? Do you want to develop a research program & teaching skills preparing you for a position at a college or small University? Our new NIH grant will support those goals 1/n Please share
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NanoTag Biotechnologies @nanotag.bsky.social · 28/09/2026
✨🔬 Want to see our JF635b-conjugated #nanobodies in action? 📄 Check out the recent NanoLetters paper by Samrat Basak @samratb.bsky.social, Roman Tsukanov @romantsukanov.bsky.social, Felipe Opazo @opazo.bsky.social et al., doi.org/10.1021/acs....
doi.org
Self-Blinking Dye Restores Efficient Use of Nanobodies in Single-Molecule Localization Microscopy
Abstract. Direct stochastic optical reconstruction microscopy (dSTORM) relies on controlled fluorophore blinking to achieve nanometer-scale resolution, yet
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Reposted by Felipe Opazo
Christophe 🔬 Leterrier @christlet.bsky.social · 25/09/2026
Here's a balanced account of where the microscopy community stands on AI use by this year's Small World in Motion winner. My main issue is that no one seems to have seen the original data despite requests (no mention of it being reviewed by the organizers) www.the-scientist.com/allegations-...
the-scientist.com
Allegations of AI Use in Nikon Competition Spark Controversy | The Scientist
Microscopists raise concerns about inappropriate AI usage in Nikon's microscopy contest-winning video. Nikon stands their ground, citing no policy breach.
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BIOspektrum @claudialudy.bsky.social · 24/09/2026
Bei einer neuen Markierungsmethode werden fluoreszierenden Marker nicht anhand ihrer Farbe, sondern ihrer Leuchtdauer unterschieden. Dadurch lassen sich mit Standardmikroskopen acht verschiedene Proteine in einer Zelle gleichzeitig sichtbar machen. @opazo.bsky.social pubs.acs.org/ancac3/artic...
pubs.acs.org
Nanobodies Equipped with HaloTag Enable Scalable Fluorescence-Lifetime Multiplexing
Abstract. Fluorescence lifetime (FL) is a robust strategy for multiplexed imaging; however, variability in fluorophores’ local microenvironment when used o
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Saravanan Palani @syncellbiolab.bsky.social · 10/09/2026
🚨 Our new @elife.bsky.social study dissects how a dividing cell keeps its septin scaffold and its contractile ring in step. 👉 elifesciences.org/articles/106... Spearheaded by @bindubhojappa.bsky.social #cellbiology #celldivision #septins #yeast #kinases
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Felipe Opazo @opazo.bsky.social · 04/09/2026
This new paper 🥂, just published in ACS Nano @pubs.acs.org s.acs.org, needed physicists, chemists, and biochemists in the same room! 🤓 Running out of colors in your #microscopy experiments? We developed #NanoFLex, which encodes protein identity by leveraging #nanobodies and #fluorescence lifetime
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NanoTag Biotechnologies @nanotag.bsky.social · 02/09/2026
#NanoTag mindset: 𝗡𝗼 𝗦𝗹𝗼𝗴𝗮𝗻𝘀. 𝗝𝘂𝘀𝘁 𝗦𝗰𝗶𝗲𝗻𝗰𝗲. We started in academia, developing #nanobodies for our own research - and we still approach our reagents as scientists: develop in-house, test thoroughly, characterise and share the data 📄. Because only reliable tools move #science forward 🚀
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Roman Tsukanov @romantsukanov.bsky.social · 27/08/2026
New publication alert 🚨 Our Nano Letters paper on self-blinking dyes with nanobodies for robust SMLM is now Open Access! pubs.acs.org/nalefd/artic... Commercial Nb–JF635b probes are available from @nanotag.bsky.social. Joint effort of amazing collaborators, inspired by the great @opazo.bsky.social
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robmoon.bsky.social @robmoon.bsky.social · 03/07/2026
We love using mNeonGreen for live but have failed to find good reagents for things like expansion microscopy. This is a fantastic addition to the toolbox. Great work!
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NanoTag Biotechnologies @nanotag.bsky.social · 03/07/2026
Proud of our team and grateful to everyone who contributed to this journey. This paper reflects our shared passion for science and our commitment to developing reliable research tools for the scientific community. 🦙💚🔬
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Joachim Goedhart @joachimgoedhart.bsky.social · 01/07/2026
A High-Affinity Nanobody Recognizing mNeonGreen Enables Versatile Biochemical, Cellular, and in vivo Applications. by Steffen Frey and team: www.biorxiv.org/content/10.6...
mNeonGreen Selector: a pulldown resin for mNeonGreen-fusion proteins. (A) Sketch of mNG
Selector binding mNG fusion proteins. (B, C) Immunoprecipitation from E.coli lysate in the presence (B) or
absence (C) of target protein (mNG-Halo fusion, arrowhead). Proteins were eluted in boiling SDS sample
buffer. mNG Selector enables highly specific one-step capture of mNG-tagged proteins. (D) Binding assay to
evaluate the cross-reactivity of the mNG Selector to other recombinant fluorescent proteins. Shown is a figure
of the equilibrium bound fraction of each indicated fluorescent protein prior to buffer washing. (E)
Compatibility of mNG Selector to various reagents applied during binding (top panel) or during washing steps
(lower panel). mNG fluorescence is lost in the presence of 2 M Gua-HCl. The symbols below each row indicate
binding efficiency: ++: strong binding, + binding, -: no binding
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Felipe Opazo @opazo.bsky.social · 02/07/2026
New paper! 🚀 A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science. We hope you find it useful! 💚
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Sasha | Kai Dao 🔬 @kaidaoscience.bsky.social · 19/06/2026
8 hours. That’s all fish need to heal wounds 🐟⏱️ Why are they so fast compared to humans? I break down the science of rapid regeneration in a microscopy story 🔬 Watch here: youtu.be/Mt9f61adaP0 #FluorescenceFriday #Microscopy
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Saravanan Palani @syncellbiolab.bsky.social · 12/06/2026
Our new paper in @plosbiology.org introduces IntAct-U-ExM enabling isoform-specific imaging of actin at nanoscale across yeast, mammalian cells & primary neurons. Fabulous work spearheaded by @anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...
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Chromatin labeling and imaging group @andimaging.bsky.social · 09/06/2026
Make HDAC6 visible again! 6SiR-C3-NextA is a selective far-red probe for imaging endogenous HDAC6 in living cells across diverse mammalian cell types. Done by @vanthang.bsky.social at @mpi-nat.bsky.social Now in JACS @pubs.acs.org pubs.acs.org/doi/10.1021/...
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Reposted by Felipe Opazo
Mario Del Rosario @mariodelr.bsky.social · 05/06/2026
Resolution isn't just optics. Structural resolvability also depends on probe size, linkage error, labelling efficiency, steric hindrance and, molecular organisation. VLab4Mic models all of these starting from PDB or AlphaFold structures
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Florian Jug @florianjug.bsky.social · 05/05/2026
It is finally out there in published form. 🎉 #MicroSplit (with an 𝕊) #Microscopy 🔬 #AI 🤖 www.nature.com/articles/s41... And no paywall... so... enjoy the read! 💸 👋 This is where the post could end... but I will have to go on for a bit below... 😉 🧵
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Ewerslab @ewerslab.bsky.social · 05/05/2026
New paper out! Left-handed DNA cannot bind to natural, right-handed DNA. This leads to lower background if it is used as a probe in DNA-PAINT. Using Fluorigenic DNA-PAINT with left-handed DNA allows for low background, fast 3D DNA-PAINT! Congrats, Bas! skeetorial: 1/x pubs.acs.org/doi/full/10....
pubs.acs.org
Accelerated Left-Handed DNA-PAINT Using Fluorogenic Probes
Single-molecule localization microscopy (SMLM) techniques offer nanometer-scale resolution by stochastically localizing individual fluorescent molecules. Among these, DNA-mediated point accumulation f...
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Subhojit Roy MD, PhD @roylab-ucsd.bsky.social · 18/04/2026
There is still time to apply. I'm now looking for someone who likes imaging, for a project like this: www.cell.com/neuron/fullt...
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NanoTag Biotechnologies @nanotag.bsky.social · 17/04/2026
📣 Neuroscientists – something NEW for you! NanoTag expands its portfolio of neurobiological #rHcAbs 🧠. Nanobodies targeting #SYT1 and #CNPase are now available as Mouse or Rabbit IgG Fc fusions – ideal for ICC and IHC 🔬. Learn more 👉 bit.ly/4cKITOd #Neuroscience #RecombinantAntibody
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Nadja Simeth @nadjasimeth.bsky.social · 13/04/2026
🚀 Just published! In PhD work by first author Dongchen Du, we’ve developed a simple, byproduct-free labeling strategy using imidazopyridinium dyes formed in situ from isoquinolines! onlinelibrary.wiley.com/doi/full/10.... #Fluorescence #Bioconjugation #ChemicalBiology #InSituLabeling
onlinelibrary.wiley.com
In Situ Construction of Imidazopyridinium Fluorescent Labels for Bioconjugation
Chromophore engineering of isoquinoline aldehydes generates TICT-based fluorogenic dyes for in situ labeling of diverse (bio)molecules with large Stokes shifts compatible with cell imaging.
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Felipe Opazo @opazo.bsky.social · 04/03/2026
New preprint out! In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...
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Guillaume Jacquemet @guijacquemet.bsky.social · 11/02/2026
Delighted to share our latest preprint "Filopodia-mediated trans-endocytosis" www.biorxiv.org/content/10.6... Work driven by many people including Hanna, Marcela, Sujan, Marie-Catherine, Anna and Monika. Fantastic collaboration with Johanna Englund, Emilia Peuhu, and Eija Jokitalo
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Brandán Pedre @brandanium.bsky.social · 09/02/2026
🧪🧬 New preprint! What if the same biosensor could reveal how cysteine is regulated across life domains? We introduce Cystector, a genetically encoded fluorescent biosensor for real-time cysteine monitoring in living cells. www.biorxiv.org/content/10.6... 🧵A thread ⬇️
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Eugene Katrukha 🇺🇦 @ekatrukha.bsky.social · 24/12/2025
Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render) @zeiss-microscopy.bsky.social
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Eugene Katrukha 🇺🇦 @ekatrukha.bsky.social · 22/12/2025
We also tried it recently and indeed, it blinks nicely without any smelly buffers or extra stuff added. Makes SMLM sample preparation a breeze. I hope soon new additional colors will be discovered, apart from the far red.
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NanoTag Biotechnologies @nanotag.bsky.social · 22/12/2025
🎄 A glimpse into #NanoTag Christmas dinner - Glühwein outdoors, a shared dinner, and Schrottwichteln 🎁♻️ where funny, used items are exchanged and find a new home. The past year has been special for us as we celebrate 10 years. Grateful for our team, customers, and partners. Here’s to what’s next 🥂
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Felipe Opazo @opazo.bsky.social · 22/12/2025
✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
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Felipe Opazo @opazo.bsky.social · 17/10/2025
Happy to tell that I got my tenured professorship at the University Medical Center in Göttingen 🇩🇪! Grateful for my students, mentors, international collaborators, the @nanotag.bsky.social team, and my family (dog included 🐾). Motivated to keep pushing science, innovation, startups, and education 🌍✨
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NanoTag Biotechnologies @nanotag.bsky.social · 07/10/2025
#NanoTag joins the Life Science Ecosystem at Nordic Life Science Days #NLSDays2025 in Gothenburg!🇸🇪 Meet our CEO Hansjörg Götzke at the Booth B03:46 (German Pavilion), and discover our sdAb platforms🦙, protein production services, and research tools🔬advancing #LifeScience & #Biomedicine.
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Felipe Opazo @opazo.bsky.social · 28/09/2025
Fantastic talk of @ewerslab.bsky.social with ExM data on Septins and Unroof Cells in #GEF25!
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NanoTag Biotechnologies @nanotag.bsky.social · 25/09/2025
🦙 NanoTag at #SingleDomainAntibodies Conference 2025! Our CEO Hansjörg Götzke will be in Utrecht sharing how our immune & synthetic sdAb platforms drive advances in research, diagnostics & therapeutics. Our founder Felipe Opazo @opazo.bsky.social will also be there to connect & share insights.
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NanoTag Biotechnologies @nanotag.bsky.social · 17/09/2025
#ExM turned 10 this year - just like #NanoTag! 🎉 Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging. See you in Göttingen! #GEF25
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Joost Snijder @joostsnijder.bsky.social · 04/09/2025
Prefusion gB of HSV bound with a neutralizing nanobody www.nature.com/articles/s41...
nature.com
A nanobody specific to prefusion glycoprotein B neutralizes HSV-1 and HSV-2 - Nature
A neutralizing nanobody specific to the prefusion conformation of herpes simplex virus glycoprotein B has cross-species activity and offers insights into virus neutralization, possible immunogens and ...
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NanoTag Biotechnologies @nanotag.bsky.social · 29/08/2025
🔬Wrapping up #SMLMS2025 @smlm-symposium.bsky.social in Bonn! Thanks to everyone who stopped by our booth! It was great connecting and sharing how our #sdAb-based solutions enhance precision, simplify #Multiplexing, and streamline workflows in #SuperResolution microscopy. See you next year! 🦙
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André Nadler @nadlerlab.bsky.social · 21/08/2025
Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism. www.nature.com/articles/s41...
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Felipe Opazo @opazo.bsky.social · 11/08/2025
🧬 New paper out! We explored why #antibodies have kept their 2 binding sites. Turns out, this “divalence” is crucial for B-cell receptors to sense antigen clustering and trigger immune responses, especially for weak or low-density antigens. Check full text: doi.org/10.1038/s414...
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Guillaume Jacquemet @guijacquemet.bsky.social · 01/07/2025
- A postdoc to work on cancer extravasation (4-year project). - A postdoc help in completing Filopodia-related lab projects (2-year). - Multiple Marie Curie postdoc positions that will open in the fall. - We will open a 5-year position to cover my teaching (70% research and 30% teaching).
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NanoTag Biotechnologies @nanotag.bsky.social · 11/06/2025
🌞🦙 Summer makeover? You bet. Our alpacas ditched the fleece to stay cool – maybe your antibodies should too. sdAbs are high-performance tools made of just the antigen-binding domain (∼15 kDa, 3 nm). 🔬🧪Perfect for IF, super-resolution microscopy, IP & more. Browse our #nanobodies: nano-tag.com/shop/
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NanoTag Biotechnologies @nanotag.bsky.social · 04/06/2025
💬Thank you, Dr. Maja Klevanski @majaklevanski.bsky.social (University of Heidelberg), for the great feedback on our recombinant anti-SARS-CoV-2 S1 antibody (rHcAb)! 😊 🔬 Applied in #dSTORM to study virion–cell interactions under antiviral conditions. Learn more about sdAb–Fc fusions 👉 bit.ly/3xdhYsn
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NanoTag Biotechnologies @nanotag.bsky.social · 28/05/2025
🤹‍♀️ Still juggling primary antibodies from different species? Still stuck in sequential staining? ⏱️Switch to Smart Secondaries®🦙: ✅OneStep-IF ✅Same-species #multiplexing ✅Higher precision & reproducibility! Choose smart 👉 bit.ly/3UZxRMI #SecondaryNanobody #Microscopy #Immunofluorescence
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NanoTag Biotechnologies @nanotag.bsky.social · 20/05/2025
@abberior.rocks — and NanoTag Biotechnologies rocks with them! 🤘 Smart Secondaries® 🦙now conjugated to abberior STAR dyes 🌈. Discover brighter, sharper, #STED -ready, species-independent #multiplexing & one-step IF. 🔬🤩 #SecondaryNanobodies #SuperResolution #FluorescenceMicroscopy
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Saravanan Palani @syncellbiolab.bsky.social · 16/05/2025
Preprint: IntAct-U-ExM enables robust, isoform-specific expansion microscopy of actin networks in yeast and mammalian cells. A cool study led by @anubhavdhar.bsky.social in collaboration with Sudarshan and Deepak Nair. www.biorxiv.org/content/10.1...
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NanoTag Biotechnologies @nanotag.bsky.social · 13/05/2025
A new study by @jungmannlab.bsky.social presents #SPINNA, a model-based tool for quantifying protein stoichiometry, oligomerization state, and spatial arrangement from super-resolution data.🔬For precise labeling of #GFP & #mCherry fusion proteins researchers used our #nanobodies 😊 📄 bit.ly/4jTHfes
bit.ly
Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications
Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...
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Nikita Vladimirov @nvladimus.bsky.social · 20/01/2025
Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!
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NanoTag Biotechnologies @nanotag.bsky.social · 06/05/2025
Fun fact: Alpacas love dust baths! 🦙💨 Spring at our farm means blossoms, sun & lots of happy rolling in the sand 🌸☀️ Just like our alpacas enjoy a refreshing dust bath, our clients enjoy smooth #sdAb discovery. Let’s roll into new discoveries together 🙃! #NanoTag #VHH #nanobody #AlpacaAntibodies
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Luciano A. Masullo @lumasullo.bsky.social · 07/05/2025
Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩 We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨
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