The endoplasmic reticulum (ER)-Golgi interface is a dynamic trafficking hub maintained in part by TANGO1, a scaffolding protein that coordinates proteins and membranes at ER exit sites (ERES). TANGO1 ...

All cells experience mechanical forces, yet how they increase energy production to meet the demands of force response remains incompletely understood. This

The Nobel Prize in Physiology or Medicine 2026 was awarded jointly to Karl Deisseroth, Peter Hegemann and Georg Nagel “for their discoveries concerning light-gated ion channels and optogenetics.”

Lipid droplets (LDs) are assembled at discrete endoplasmic reticulum (ER) subdomains whose local phospholipid composition, rather than their protein content alone, determines whether a LD nucleates an...

Protein misfolding in the endoplasmic reticulum (ER) of podocytes/glomerular epithelial cells (GECs) induces ER stress and contributes to the pathogenesis of glomerular disease. Activation of IRE1α an...

Li et al. identify nitrate-responsive Sialin2 as a regulator that couples stress granule assembly to autophagy-linked recovery, thereby preserving condensate reversibility and limiting disease-associa...

Autophagic degradation of parts of the nucleus (nucleophagy) and endoplasmic reticulum (ER-phagy) utilizes the selective autophagy receptors, Atg39...

3-Phosphoinositides are essential cellular lipids regulating health and disease. Among them, phosphatidylinositol-3,5-bisphosphate [PI(3,5)P2] remains the least understood. Using a newly developed rat...

Mouse experiments show that Alzheimer’s disease and corticobasal degeneration tau filaments retain their distinct structures during templated seeding, supporting strain-specific tau propagation a...

Proteomic and phosphoproteomic analyses of lysosomes identify a nutrient-independent regulatory arm of mTORC1 signalling that mediates lysosomal adaptation to stress and whose deregulation underl...

Constitutive lysosomal degradation of cytosolic proteins is fundamental to proteostasis, but how these substrates are delivered into the lysosomal lumen remains incompletely understood. In mammals, th...

Cargo has traditionally been viewed as a passive substrate of coat-mediated transport. Cryo-EM reveals that cargo containing tandem sorting motifs directly promotes higher-order adaptor assembly by br...

Lennartz et al. evaluate lipid enrichment in clathrin-coated pits and observe that the differential lipid partitioning into clathrin-coated pits is largely driven by the lipid asymmetry of the plasma ...

Dietary restriction (DR) protects against metabolic disease, extends lifespan, and is associated with remodeling of tissue reactive oxygen species (ROS). ROS control biological adaptation through reve...

Organelles form an interconnected network whose morphology, positioning and interactions reflect cellular state. However, reproducibly quantifying these organelle phenotypes across large cell populati...

Sydir et al. identify an ESCRT-independent pathway for nuclear envelope assembly in S. japonicus that involves the conserved proteins, Vid27 and Alx1. This

The endoplasmic reticulum (ER) is the primary site of eukaryotic membrane protein synthesis and quality control, which largely relies on ER-associated degradation (ERAD) to eliminate aberrant nascent ...

The autophagy core machinery mediates the enclosure of cytosolic cargo destined for degradation in the lysosome. The Atg9-Atg2-Atg18 complex coordinates phagophore expansion via directed lipid transfe...

Meiring, Mahapatra et al. show that expanded microtubule lattice conformations, such as those induced by small-molecule taxanes, can lead to the release of GEF-H1 and thus induce RhoA activation.

The B cell receptor (BCR) drives the differentiation of naive B cells into activated plasma cells that produce antibodies. The BCR consists of a plasma membrane-bound surface immunoglobulin (sIg) that...

Reactive astrocytes emerge as a primary consequence of TSC2 loss in tuberous sclerosis complex, implicating glial dysfunction as a primary driver of pathogenesis and highlighting the astrocytes as pot...

The endoplasmic reticulum (ER) extends throughout neurons and regulates many functions, including neurite outgrowth, neurotransmission, and synaptic plasticity. Mutations in ER-shaping proteins cause ...

Rab GTPases control myriad molecular functions by acting as a nucleotide-dependent switch for protein-protein interactions localized in discrete subcellular regions. Finding additional molecular roles...

Lysosomes degrade extracellular and intracellular materials targeted for disposal. Lysosomes display an acidic milieu established by the V-ATPase that promotes this hydrolytic activity and powers memb...

To integrate diverse cellular function and coordinate homeostasis, the endoplasmic reticulum (ER) communicates with all other intracellular compartments through physical interfaces termed membrane con...

Ensuring quality and maintenance of mitochondria within eukaryotic cells is important for cellular fitness. One critical pathway that needs to be tightly controlled to achieve this is mitophagy, which...

Lysosomal enzymes are synthesized in the Endoplasmic Reticulum (ER) and transported to lysosomes to execute their functions. Deficiencies in lysoso...

CRISPR-Cas provides RNA-mediated adaptive immunity, but how its first RNA-guided effector arose is unclear. In this study, we report the discovery of Viral Interference Programmable Repeat (VIPR) syst...

Cell-state diversity drives tissue adaptability, repair, and disease resilience, but capturing this complexity is a challenge. Current approaches rely on transcriptional profiling and overlook organel...

Zhao et al. develop constitutive and inducible Golgi reporter mice that enable in vivo visualization of Golgi architecture and Golgiphagy at single-cell re

The mitochondrial–peroxisomal fission adaptor MFF is identified as a selective pro-ferroptotic mediator, and the newly developed MFF–SPARK sensor enables high-content screening of ferroptosis-sen...

Ferroptosis is a regulated form of cell death driven by iron-dependent and unrestrained lipid peroxidation, which generates phospholipid hydroperoxides that cause membrane rupture. Oxidized phospholip...

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Damaged mitochondria are cleared by lysosomes. Here, authors show that ER-to-lysosome cholesterol transport preserves lysosomal degradative function during mitophagy, enabling efficient recycling of l...

Two studies show that the endoplasmic reticulum-associated protein CLN8 catalyses the acyl CoA-dependent acylation of glycerophosphoglycerol, producing lysophosphatidylglycerol, which is then converte...

Tunneling nanotube-like (TNT) connections allow mitochondrial exchange between tumor cells and its tumor microenvironment. Here, the authors show that TNT connections mediate bidirectional mitochondri...

Accessible cholesterol, the pool of membrane cholesterol with high chemical activity, regulates vital processes including vertebrate development and pathogen evasion. The mechanisms that govern plasma...

Microtubules are key components of the eukaryotic cytoskeleton involved in vital functions in virtually every cell. Among the emerging molecular mechanisms to adapt microtubules to their diverse funct...

Xu, Guo et al. report suppressor screens in Caenorhabditis elegans to counteract tubulin mutants. They report several missense variants in tubulin genes that may restore microtubule architecture and d...

The ciliary transition zone (TZ) regulates ciliary proteome composition, yet its molecular architecture, protein content, and contribution to motile ciliopathies remain poorly defined. We applied in s...

The cell cycle is tightly regulated by checkpoint mechanisms that ensure faithful duplication and segregation of the genome. Here, we induced cell-cell fusion between mitotic and interphase cells to s...

Yang et al. reveal that the ARLA1A-NAC089 module couples ER phagy with the UPR in plants. This dual regulatory axis fine-tunes cell survival versus death under stress, providing a mechanistic framework for maintaining ER homeostasis and enhancing environmental resilience.

Agarwal et al. introduce MitoSpace, a self-supervised model trained on 4D lattice light-sheet microscopy data. The model resolves drug-induced mitochondrial phenotypes without labels, predicts membran...