Lennartz et al. evaluate lipid enrichment in clathrin-coated pits and observe that the differential lipid partitioning into clathrin-coated pits is largely driven by the lipid asymmetry of the plasma ...

Radulovic et al. show that phosphatidylinositol 3-phosphate (PI3P), produced by the phosphatidylinositol 3-kinase PIK3C3, is formed on ER domains after lysosome damage. ER domain PI3P helps to recruit...

Acidification of lysosomes, endosomes and the Golgi underpins organelle-specific functions within the endomembrane system. This process is driven by vacuolar-type H + -ATPases (V-ATPases), proton pump...

Inhibition of Pikfyve, a kinase generating PI(3,5)P2, abolishes CHMP4B (an ESCRT-III subunit) recruitment to lysosomes, and leads to an accumulation of STING vesicles and sustained signaling. Our resu...

Endoplasmic reticulum autophagy (ER-phagy) is a selective autophagy pathway in which receptor proteins target ER membranes and proteins for degradation, yet its role in Alzheimer’s disease (AD) remain...

Compartmentalization is central to coordinate complex biochemical processes in eukaryotic cells. Proteins governing organelle function and proteome co…

This study describes the cell-type-specific lysosomal proteome across the major brain cell types and identifies SLC45A1 as a neuron-specific lysosomal sugar exporter linked to lysosomal storage disease.

Coupling an optogenetic lipid-modifying enzyme with proximity labeling reveals protein networks and mechanisms regulating lipid homeostasis in the membranes of target organelles.

Proper neuronal development and function rely on the polarized sorting of transmembrane proteins (TMPs) to their distinct somatodendritic or axonal domains. Most TMPs follow a conventional secretory p...

Brain function requires precise spatiotemporal regulation of the neuronal proteome. To allow adaptation of the proteome in distal outposts of neurons,…

It is unclear how new lysosomal proteins are properly delivered in polarized neurons. Here, the authors developed a method combining the synchronization of protein release from the endoplasmic reticul...