Chromatin organizes nuclear DNA, but whether it actively regulates whole-cell mechanics remains unknown. Here, we used NETosis to show that chromatin decompaction within the nucleus increases plasma membrane tension and cell volume, establishing chromatin as a regulator of cell mechanics.

Cellular lipid homeostasis requires mechanisms that detect subtle changes in lipid abundance and trigger rapid, coordinated responses. The INSIG/SCAP/SREBP2 pathway provides a central feedback system ...

An alkyne-tagged ethanolamine analog (AlkEA) enables bioorthogonal, metabolic labeling of endogenous phosphatidylethanolamine (PE) and its protein modifications. AlkEA is incorporated into PE via the...

Cellular lipid homeostasis requires mechanisms that detect subtle changes in lipid abundance and trigger rapid, coordinated responses. The INSIG/SCAP/SREBP2 pathway provides a central feedback system ...

Nature - Human cells cultured in microgravity produced fewer mitochondrial proteins.

VPS13C, whose dysfunction causes Parkinson’s disease, adopts a lipid-transfer-inactive conformation in the cytoplasm and relies on calmodulin for proper folding and localization to membrane surfaces.

Long associated with the regulation of the endolysosomal pathway, the authors show that the PIKfyve lipid kinase extends its sphere of influence to modulat

Nature Communications - Not all components of the lysosome damage response pathway have been defined. Here, the authors discover that the bridge-like lipid transport protein VPS13C senses lysosomal...

The new EMBO Members and Associate Members are recognized for their outstanding achievements in the life sciences

Thiam and Carpentier review seipin as a conformationally dynamic lipid rheostat that steers lipid fate and preserves cellular metabolic balance.

Speakers invités: Giovanni D’Angelo (EPFL, Lausanne, Suisse) Christophe Leterrier (INP, Marseille, France) Miriam Stoeber (University of Geneva, Genève, Suisse)

The ER's continuous tubular network is maintained by ER-shaping proteins whose mutation or dysregulation contributes to neurodegenerative diseases. Here, we show that ER morphology sets the speed of ...

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Here, Deol et al. use genetic screens in gene-edited reporter cell lines to identify regulators of ferroptosis suppressor protein 1 (FSP1) expression and stability. They show that vitamin B2 metabolis...

CRISPR–Cas9 screening identifies CLCC1 as a factor that increases neutral lipid flux to prevent hepatic steatosis and promotes nuclear pore complex assembly by promoting membrane bending and fusi...

The SNARE VAMP7 is essential for an unconventional secretory pathway based on late endosomal secretion. Here the authors show that this secretion functions as an organelle quality-control and stress-c...

Lysosomes are essential in maintaining cellular health. Endocytic lysosome reformation (ELR) regenerates functional lysosomes following degradation of endocytic cargo, yet the mechanisms driving this process remain largely unknown. Here, we define the molecular machinery underlying ELR. We find that unlike autophagic lysosome reformation (ALR), ELR proceeds independently of mTOR and dynamin 2, but requires the mitochondrial fission GTPase DRP1. DRP1 mediates scission of endolysosomal tubules at contact sites with the endoplasmic reticulum (ER) and mitochondria. Disruption of DRP1 function or ER endolysosome contact results in elongated tubules, indicating defective lysosome reformation. Moreover, mitochondrial activity is essential for tubule initiation, and Ca2+ transfer from endolysosomes to mitochondria is crucial for ELR onset. Our findings reveal a dual role for mitochondria in ELR: first in ELR initiation and second in DRP1-dependent tubule fission at ER-mitochondria-endolysosome tripartite contact sites, uncovering the previously unappreciated role of mitochondria in endolysosome remodeling and fission. ### Competing Interest Statement The authors have declared no competing interest. Swiss National Science Foundation, 320030-231859, 310030-197779 University of Basel, https://ror.org/02s6k3f65

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Coupling an optogenetic lipid-modifying enzyme with proximity labeling reveals protein networks and mechanisms regulating lipid homeostasis in the membranes of target organelles.