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The amoeba Incendiamoeba cascadensis demonstrates that eukaryotic life can withstand temperatures beyond what was thought possible and sheds light on molecular strategies for survival in extreme heat.

Across eukaryotes, the nuclear envelope exhibits distinct remodelling strategies during mitosis: complete breakdown (open mitosis), partial disruption (intermediate), or full retention (closed). Howev...

Animal cells extensively remodel their cytoskeleton during differentiation and can notably switch between two major motility modes: flagellum-based swimming and actin-based crawling. We previously sho...

Bhaskar Naidu and Vera Lillo et al. show that extracellular mechanical forces reciprocally regulate Golgi function. By linking cell adhesion and spreading,

Using in situ cryo-electron tomography, researchers found a unique ‘arm’ domain on the small subunit of Chlamydomonas chlororibosomes. This discovery suggests that chlororibosome structure is more evo...

The authors show that persistent ultrafine DNA bridges become coated by membranes and the ESCRT-III proteins CHMP1B and IST1, which protect the genome within from damage.

The manchette is a transient microtubule (MT)-based structure that is vital for the correct shaping of sperm during spermiogenesis. Throughout spermiogenesis, the manchette retains structural integrity for several days, raising the question of how its MTs are regulated. Here, using cryo-electron tomography of manchettes isolated from rat testes, we find that manchette MT ends are structurally diverse. We show that the MT-binding protein CLASP2 is present throughout the manchette and likely regulates both MT ends. Using cryo-electron microscopy single particle analysis and super-resolution microscopy, we reveal that SPACA9 and MNMIP1 (SH3D21) bind to the seam of manchette MTs from the luminal side. SPACA9 binds to both α- and β-tubulin of protofilament 1 but does not interact directly with protofilament 13, while MNMIP1 binds directly to protofilament 13. MNMIP1 further extends and threads through the MT lattice at the seam. Our study reveals a novel seam MT inner protein complex with a unique binding mode, providing a plausible explanation for MT regulation that maintains manchette integrity over an extended period.

The nitrilotriacetic acid group coordinated with nickel, NTA(Ni), is the standard chelator for purifying fusion proteins containing poly-histidine tags. The development of lipids with NTA(Ni) headgrou...

DNA damage in the tardigrade Hypsibius exemplaris elicits distinct cellular outcomes depending on replication status. While non-replicating cells tolerate genotoxic stress, constitutively replicating...