The actin-related protein 2/3 (Arp2/3) complex is a central nucleator of branched actin filaments, essential for numerous cellular processes. Using superresolution imaging, we demonstrate that Arp2/3 ...

The sequential assembly of over 50 proteins during clathrin-mediated endocytosis (CME) is well documented in yeast. However, the mechanisms governing this ordered recruitment remain poorly understood....

The Golgi apparatus expands during differentiation and increased secretory demand, yet the transcriptional mechanisms governing its biogenesis remain poorly defined. To investigate this process, we de...

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AP3 generates clathrin-free carriers on endosomes by organizing via ARF1 dimers into elongated membrane-deforming arrays.

This Perspective discusses the mechanisms underlying both the formation and scission of caveolae, with a focus on highlighting the role of specific lipids in regulating and coupling these processes.

The SNARE VAMP7 is essential for an unconventional secretory pathway based on late endosomal secretion. Here the authors show that this secretion functions as an organelle quality-control and stress-c...

Talin has been believed to be indispensable for integrin activation. Here, the authors show that the curvature-sensing protein FCHo2, not talin, enables inside-out activation of integrin ɑvβ5 in curve...

Chemical synapses are fundamental units for the transmission of information throughout the nervous system. The cytoskeleton allows to build, maintain and transform both pre- and postsynaptic contacts,...

Targeted nanoparticles have the potential to revolutionize therapeutics for medical applications. Here, we demonstrate the utility of a flexible precision nanovesicle delivery system for functional de...

The microtubule cytoskeleton is the backbone of neuronal morphogenesis, driving the development of the dendrites and axon, and supporting trafficking to distant compartments. How neuronal microtubules...

An epidermal membrane periodic scaffold protects axons from damage.

Extracellular vesicles are released from almost all cell types and mediate intercellular communication by delivering proteins, nucleic acids and lipids. This Review examines extracellular vesicle upta...

Scientific Reports - Combining the CryoCapsule, HPM live µ and R221 conductive resin to track endosome from live imaging to electron microscopy using high-pressure freezing

Benito-Martínez, Salavessa and colleagues show that keratin intermediate filaments and microtubules control the three-dimensional perinuclear position of melanin-containing organelles, shielding the D...

The Mant-ATP assay is a simple and accessible means for rapid quantitative assessment of the ratio of super-relaxed myosin to disordered relaxed myosin. This protocol provides a standardized methodolo...

Background Myotonic dystrophy type 1 (DM1) is a multisystemic neuromuscular disorder characterized by CTG repeat expansion in the 3′ untranslated region of the dystrophia myotonica protein kinase co...

This time on The Microscopists, we're joined by Christophe Leterrier, team leader at NeuroCyto in Marseille and director of a Nikon Center of Excellence for super-resolution microscopy.Christophe r...

Myelination of CNS axons requires oligodendrocytes to undergo extensive morphological changes by producing large amounts of myelin membrane with defined protein composition and structure. The formatio...