Linking cellular phenotype to genotype and gene expression is a central goal in biomedical research. Live-cell imaging captures dynamic cellular phenotypes, whereas single-cell sequencing measures gen...

Current genetic screens face a trade-off between scaling to large mutant libraries and measuring information-rich phenotypes. SPARCS automates laser microdissection to enable pooled forward genetic screens for complex image-based phenotypes at scale. By physically recovering individual mutants, SPARCS supports multimodal profiling of hits beyond genotyping, including via mass spectrometry-based proteomics.

Many epigenetic regulators play essential roles in animal development, but precisely how they function across different cell types without clear sequence preferences remains a foundational challenge. ...

This annual meeting takes place at Munich (DE) providing an instrumental platform to discuss the recent findings on epigenetics with the local community.

Simulations integrating micro-C and imaging data provide a coherent view of chromatin organization from nucleosomes to clutches to domains, revealing that most regions form compact domains but short r...

Transposable elements mobilize within and occasionally between genomes, including from host to virus. We identified two insertions of the human BC200 noncoding RNA gene in the poxvirus molluscum conta...

Cohesin-mediated loop extrusion limits sister-fork coupling and tethers nearby replication forks under replication stress, promoting fork reversal and slowing fork progression to safeguard genome...

By tethering a protein of interest to anti-CRISPR, protein degradation is transformed into amplified and programmable transcriptional outputs via Cas-based transcription factors, with sensitivity to p...

Nature Reviews Molecular Cell Biology - Single-molecule genomics methods are used to study the activity of regulatory factors on individual DNA molecules genome-wide, thereby enabling...

View details and apply for this Postdoctoral Training Fellow - Chromatin Biology and Epigenetics vacancy in Chelsea . Salary: Salary range £45,600 - £51,450 per annum. Dependent on re...

The genomic regions regulating gene expression are often themselves transcribed into a variety of noncoding RNAs (ncRNAs). However, the regulatory roles of this noncoding transcription remain largely ...

The genomic regions regulating gene expression are often themselves transcribed into a variety of noncoding RNAs (ncRNAs). However, the regulatory roles of this noncoding transcription remain largely ...

Biayna, Baranovskii and colleagues report that intron-retained RNAs derived from cell cycle- and genome maintenance-related genes are pooled at the periphery of nuclear speckles and undergo synchronou...

Cryo-EM and genetic analyses reveal that the SWI/SNF chromatin remodeler is an integral component of the transcription pre-initiation complex. The structures demonstrate how SWI/SNF facilitates transcription initiation through stabilization of PIC organization and concerted remodeling of the +1 nucleosome.

DNA replication generates sister chromatids with identical sequence, yet its mechanism is fundamentally asymmetric: Chromatids inherit strands of opposite orientation, whereas forks synthesize new str...

Najia, Le, Borrajo et al. establish “cellular self-reporting,” a technology to export molecular analytes from cells in virus-like particles. The authors demonstrate how mRNA export enables longitudinal transcriptome-wide profiling of living cells by sampling cell media. This platform transcends the destructive nature of conventional transcriptomic analysis methods and reveals gene expression dynamics in complex biological systems in situ.

More than twenty years ago, the FANTOM consortium uncovered that mammalian genomes are pervasively transcribed, revealing multitudes of RNAs with unknown functions. A subset of these transcripts has s...

Knowledge of the spatial location of a gene is necessary to understand its epigenomic regulation.

Transcription factors (TFs) differ in their ability to interact with nucleosomes, and most TFs need other TFs to locate their motifs in the human genome. Here, Xiao et al. show that two ELF2 proteins are able to unwind DNA from nucleosomes and access all of their motifs in the genome.

Bulk transcriptome and proteome correlate only modestly, but this has not been investigated in the same cell or across cell-state changes. Here we introduce a scalable technology that quantifies thous...

Lebrec et al. present a FRET-based cGAS reporter and show that cGAS activation by genotoxic stress is rare and uncoupled from micronuclei. The study implicates R-loop structures and subtypes of chroma...