Wang et al. introduce HoTs, self-blinking DNA probes for live-cell super-resolution chromatin imaging. Compatible with both STORM and MINFLUX microscopy, HoTs capture chromatin organization across live cells, animal tissues, and clinical tumor samples, opening new opportunities for studying genome architecture in health and disease.

Ning Xu - Abnormal beating of airway cilia from a child with Primary Ciliary Dyskinesia (PCD), a genetic disorder affecting respiratory function.

Lab students were supposed to ID a mild germ. They all identified a deadly pathogen.

We show that, in a genomic locus with minimal complexity, the distance between promoter and enhancer modulates the frequency of clustered transcriptional bursts. Thus, besides the nucleotide sequence,...

Springer Nature has removed two studies by Max Planck. A bot may be to blame

Progressive intercellular spreading of α-synuclein (αS) is implicated in pathology initiation and propagation of synucleinopathies. However, how recipient neurons respond to incoming αS and whether these responses contribute to disease-associated early metabolic events, remains unknown. Here, using extracellular monomeric αS to model the earliest cellular response to spreading, we found that internalized αS accumulates at tri-organelle contact sites linking mitochondria, endoplasmic reticulum, and endo/lysosomal compartments. At these interfaces, αS stabilizes generally dynamic contacts and constrains their remodeling, thereby rewiring organelle communication. These effects require the acidic αS C-terminus and are not recapitulated by intracellular αS overexpression. Proteomic profiling of αS-associated mitochondria identified a contact site-enriched but quality-control-deficient state. Functionally, spreading αS impairs neuron-astrocyte mitochondrial quality control (MQC) by reducing neuronal mitochondria transfer to astrocytes, while enhancing mitochondrial import. Our findings establish organelle contact sites as critical target of spreading αS, through which rewired organelle communication impairs MQC and neuron-astrocyte crosstalk. ### Competing Interest Statement The authors have declared no competing interest.

A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...