Nature Protocols, Published online: 24 September 2026; doi:10.1038/s41596-026-01444-9SIMworks is an integrated software suite for quality-controlled augmented quantitative structured illumination microscopy (SIM), enabling both new learners and experienced users to implement best practices in SIM imaging.

Nature Protocols, Published online: 24 September 2026; doi:10.1038/s41596-026-01447-6This tutorial overviews the use of organoid technologies across a broad spectrum of applications, from investigating cell fate decisions and cell–cell interactions to studying genetic diseases, species evolution and clinical questions.

Nature Protocols, Published online: 24 September 2026; doi:10.1038/s41596-026-01445-8A protocol for screening compounds using human reporter iPS cell-derived cardiac fibroblasts to identify antifibrotic drug candidates with translational potential.

A Nature conference reimagining what it means to deliver truly personalised care in the next decade.

Nature Protocols, Published online: 21 September 2026; doi:10.1038/s41596-026-01427-wTissue expansion mass spectrometry imaging enables the mapping of lipids, metabolites, proteins, peptides and N-glycans at high spatial resolution.

Nature Protocols, Published online: 21 September 2026; doi:10.1038/s41596-026-01416-zThis Protocol uses retrograde axonal tracing and fluorescence-activated cell sorting to transcriptionally profile innervating neurons at single-cell resolution.

Nature Protocols, Published online: 17 September 2026; doi:10.1038/s41596-026-01438-7Drug self-administration studies capture the voluntary, goal-directed nature of human drug taking, allowing investigators to dissociate the behavioral and neural mechanisms that motivate drug seeking and taking from the pharmacological effects of drug exposure itself. Now, a protocol provides instructions for hardware assembly and implementation of intravenous drug self-administration in head-fixed mice.

This Collection from Nature Protocols covers metabolomics and metabolite analysis. The protocols cover key stages of metabolite research, including sample ...

A collection of proteomics protocols from Nature Protocols focussing on our most recent content.

A collection of protocols at the interface of electronic, robotic and living systems to fabricate actuators, circuits, robots, wearables and integrated ...

Sensing technologies provide us with the ability to detect changes in local chemical, biological or physical environments we would otherwise be unaware of. ...

A collection of articles covering protocols for synthesizing, processing and characterizing advanced materials, with particular emphasis on 2D materials and ...

Nature Protocols, Published online: 16 September 2026; doi:10.1038/s41596-026-01440-zThis protocol reliably generates induced pluripotent stem cells derived from hair follicle keratinocytes.

A Nature conference reimagining what it means to deliver truly personalised care in the next decade.

Nature Protocols, Published online: 15 September 2026; doi:10.1038/s41596-026-01432-zWe present a Protocol for long-term imaging of intestinal organoids, in combination with OrganoidTracker software, that enables both single-cell tracking analysis for use in dynamic cell type inference and fluorescence intensity measurements.

Nature Protocols, Published online: 11 September 2026; doi:10.1038/s41596-026-01414-1This protocol describes the generation of eight-cell embryo-like cells (8CLCs) from human pluripotent stem cells. The resulting 8CLCs exhibit transcriptional and epigenetic features resembling those of human 8C embryo cells.

Nature Protocols, Published online: 11 September 2026; doi:10.1038/s41596-026-01430-1Copper-based catalysts undergo structural changes during the course of the chemical reaction. This protocol describes how to use in situ/operando analysis using Raman, IR, XPS and XRD to understand Cu reconstruction during CO2 electroreduction.

Nature Protocols, Published online: 09 September 2026; doi:10.1038/s41596-026-01446-7This is a Perspective covering a Python toolbox for neuroscience research that enables users to fetch datasets, construct networks and null models, estimate graph metrics, fit statistical models and visualize brain images.

A Nature conference reimagining what it means to deliver truly personalised care in the next decade.

Nature Protocols, Published online: 04 September 2026; doi:10.1038/s41596-026-01422-1Here the authors provide a flexible and versatile protocol for surface functionalization of living cells through in situ dopamine polymerization that allows personalized living cells to be designed with customizable functions by tuning the surface components.

Nature Protocols, Published online: 03 September 2026; doi:10.1038/s41596-026-01434-xThe cell types forming the blood–brain barrier are underrepresented in existing single-cell studies. This protocol describes the isolation of cerebral vessels and subsequent enzymatic extraction of high-purity nuclei for downstream multiomics.

Nature Protocols, Published online: 01 September 2026; doi:10.1038/s41596-026-01429-8The physicochemical properties of transition metal dichalcogenides strongly depend on their crystal phases. This Protocol describes a salt-assisted synthesis of metastable 1T′-transition metal dichalcogenide crystals with high crystalline quality and high phase purity.

Nature Protocols, Published online: 28 August 2026; doi:10.1038/s41596-026-01442-xWe present a protocol for the fabrication of highly ordered composite films in which 2D nanosheets can be aligned and fixed, yielding high tensile strength and suitability for high-performance wearable electronics.

Nature Protocols, Published online: 25 August 2026; doi:10.1038/s41596-026-01428-9Uniform two-dimensional platelets made from synthetic polymers can range in size from 100 nm to several micrometers and have diverse applications. This Protocol describes the synthesis and crystallization-driven self-assembly of poly(ε-caprolactone)-based polymers.

Nature Protocols, Published online: 24 August 2026; doi:10.1038/s41596-026-01421-2This protocol is for the synthesis of radioafterglow nanoprobes that convert X-ray energy to near-infrared emissions with long half-life and high brightness, detectable from up to 15-cm depth.

Nature Protocols, Published online: 24 August 2026; doi:10.1038/s41596-026-01425-yMetal adsorption using metal–organic frameworks is a good way to remove and recover heavy or rare-earth metals from water in the environment. This protocol describes their design as well as assays to characterize their stability and performance.

Nature Protocols, Published online: 17 August 2026; doi:10.1038/s41596-026-01426-xThis Protocol outlines a pathologist-led, centralized workflow for processing frozen tumor tissues to generate high-quality DNA for whole-genome sequencing. It integrates cryosectioning, digital pathology, macrodissection and laser microdissection to enrich tumor content and automated DNA extraction.

Nature Protocols, Published online: 17 August 2026; doi:10.1038/s41596-026-01415-0High-resolution liquid chromatography–tandem mass spectrometry is widely used in exposomics, for example, to study metabolomic responses to environmental exposures. This Protocol describes spectra processing, statistical analysis, dose–response modeling and causal analysis using MetaboAnalyst 6.0.

Nature Protocols, Published online: 13 August 2026; doi:10.1038/s41596-026-01418-x[FeFe]-Hydrogenases catalyze H2 production and consumption. Their impact is limited by the complexity of the natural synthetic process. This protocol describes the synthesis of diiron subclusters to simplify the production of functional enzymes.

Nature Protocols, Published online: 11 August 2026; doi:10.1038/s41596-026-01407-0A printable composite material provides a practical and cost-effective route for fabricating metasurfaces on flexible or curved substrates, scalable from the UV to IR wavelengths and deployable for photonic devices.

Nature Protocols, Published online: 11 August 2026; doi:10.1038/s41596-026-01399-xAn approach for combining expansion microscopy with fluctuation-based super-resolution analysis enables the visualization of individual proteins via conventional fluorescence microscopy.

Nature Protocols, Published online: 10 August 2026; doi:10.1038/s41596-026-01417-yAntioxidant nanozymes are therapeutic candidates for oxidative diseases. Here a standardized protocol is described to characterize superoxide dismutase-like or catalase-like nanozymes and quantify their activity.

Nature Protocols, Published online: 10 August 2026; doi:10.1038/s41596-026-01406-1Here, the authors provide a protocol for a head-restrained approach in mice for intravenous and oral self-administration of drug and nondrug rewards.

Nature Protocols, Published online: 05 August 2026; doi:10.1038/s41596-026-01431-0Stabilization of metal single-atom or cluster catalysts is achieved by forming CeOx nanoislands on SiO2 through strong electrostatic adsorption method. Transition-metal precursors are added to form single atoms or clusters on these islands.

Nature Protocols, Published online: 30 July 2026; doi:10.1038/s41596-026-01423-0Overestimations in the cyclization efficiency of small, restriction enzyme-digested DNA fragments

Nature Protocols, Published online: 30 July 2026; doi:10.1038/s41596-026-01424-zReply to: Overestimations in the cyclization efficiency of small, restriction enzyme-digested DNA fragments

Nature Protocols, Published online: 28 July 2026; doi:10.1038/s41596-026-01412-3This protocol describes Core2Edge, a human glioblastoma organoid and brain slice co-culture model that recapitulates tumor infiltration and transcriptional heterogeneity, along with a light-sheet imaging approach to capture the entire model in three dimensions.

Nature Protocols, Published online: 28 July 2026; doi:10.1038/s41596-026-01419-wA microscopy approach for the use of focus-feedback for single-plane tracking of subcellular structures during prolonged live-cell fluorescent imaging sessions.

A collection of protocols covering a range of techniques for isolating, profiling, identifying and characterizing immune cells and biomarkers

Nature Protocols, Published online: 27 July 2026; doi:10.1038/s41596-026-01410-5This Protocol Extension describes a programmable tetrahedral DNA nanostructure (TDN) platform for selective cell and protein capture, using aptamer-functionalized TDN for cell capture and peptide-functionalized TDN–hydrogel for protein sequestration.

Nature Protocols, Published online: 24 July 2026; doi:10.1038/s41596-026-01385-3Polymer deconstruction, via catalytic hydrogenolysis, is an important approach for plastic recycling and upcycling. This workflow describes sequential quantitative analysis using multiple techniques to account for all of the reactants and products.

Nature Protocols, Published online: 24 July 2026; doi:10.1038/s41596-026-01408-zAn entry-level guide for understanding how to choose large language models for medical research applications, customize the prompts and fine-tune the models’ performance.

Nature Protocols, Published online: 23 July 2026; doi:10.1038/s41596-026-01411-4A protocol covering the synthesis of lipid-shelled nanobubbles, which enable an increase in sensitivity of contrast-enhanced ultrasound imaging and expand the possible applications in the clinical space.

Nature Protocols, Published online: 22 July 2026; doi:10.1038/s41596-026-01405-2Cellular lipids are known to have specific protein interactions. This protocol describes how to study the lipid–protein interactome using multifunctional lipid probes capable of photo-cross-linking and click chemistry by in-gel fluorescence or mass spectrometry.

Nature Protocols, Published online: 21 July 2026; doi:10.1038/s41596-026-01409-yThis large-scale genome structure interrogation approach uses prime editing to insert recombinase recognition sites into repetitive genomic regions. It enables thousands of precise genomic edits to be installed per cell with systematic investigation of their effects.

Nature Protocols, Published online: 15 July 2026; doi:10.1038/s41596-026-01382-6This is a Tutorial Review on advances in bioelectronic interfaces covering research in material science for biomimetic interfaces and in synthetic biology for the reconstitution of complex biological functions.