iiinova — where your digital self keeps evolving

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Juanma García-Arcos @juanmagararc.bsky.social · 10/09/2026

1/3 A few snapshots from the past intense days at the University of Helsinki for our FLIM and FCS course! We combined lectures, hands-on microscopy and analysis with plenty of questions and discussion. A fantastic group of researchers to learn with. 🔬 #FLIM #FCS #Microscopy

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🍁 Bryan Heit, PhD 🍁 @bryanheit.bsky.social · 28/08/2026

Happy #FluorescenceFriday! This is the cortical actin cytoskeleton of a macrophage imaged using eSRRF and @spirochrome.com's SPY555-FastAct_X.

image shows the web-like network formed by actin. Image is pseudo-coloured using a heatmap with red "dim" regions and orange-white "bright" regions.

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Spirochrome @spirochrome.com · 01/06/2026

Spoiler: lots of 😍 SPY555-FastAct live cell movies in there.

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Spirochrome @spirochrome.com · 01/06/2026

Today is the Day, CenSpark650 is available! Time to get those centrioles and cilia shining under the 🔬objective! spirochrome.com/product/cens... video: 20h imaging of primary cilium formation in a single RPE-1 cell stained with CenSpark650. movie credits: @cepourroy.bsky.social

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Omaya Dudin 𓂆 ¦🍉¦🦠¦🔬¦ @dudinlab.bsky.social · 28/05/2026

An amazing new tool to explore "non-models" is just about to open a new world of imagining possibilities....

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Spirochrome @spirochrome.com · 27/05/2026

📢On June 1st, we will be launching CenSpark650, the very first selective fluorescent probe for imaging centrioles and cilia in live cells.🔬 No transfection, zero genetic manipulation. Just Add & Image 1 hour later. Learn more: spirochrome.com/12-may-2026/ #Bioimaging

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Spirochrome @spirochrome.com · 04/05/2026

Casually posting this one for no particular reason! #MayTheFourth

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Spirochrome @spirochrome.com · 01/05/2026

Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆 The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes. evidentscientific.com/en/landing/i...

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Spirochrome @spirochrome.com · 01/05/2026

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Evelyn Strickland @strickland-evelyn.bsky.social · 20/04/2026

What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~ www.biorxiv.org/content/10.6...

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Spirochrome @spirochrome.com · 21/04/2026

Mesmerizing light sheet imaging of neutrophils swarming at a yeast target! The neutrophils were labeled with SPY650-DNA (yellow) and a calcium dye (blue). This movie is part of the recent manuscript by @strickland-evelyn.bsky.social that we highly recommend reading! www.biorxiv.org/content/10.6...

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Spirochrome @spirochrome.com · 16/04/2026

What a probe! Congratulations @cepourroy.bsky.social! We're happy to share that we re working hard on making CenSpark probes available to the centriole aficionados in the coming weeks!

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Spirochrome @spirochrome.com · 08/04/2026

Organoids stained with Spirochrome probes for actin and DNA! 😍

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Zhixing Chen @zhixingchen2.bsky.social · 04/04/2026

See how PKMito dyes promote mitochondrial research😀 @spirochrome.com

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Spirochrome @spirochrome.com · 01/04/2026

Beware the SPY488-FastAct rollercoaster! We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct. Find out more about SPY488-FastAct: spirochrome.com/product/spy4...

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Spirochrome @spirochrome.com · 23/03/2026

📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy! There is now a very detailed #JOVE video that explains it all step by step. Video link: dx.doi.org/10.3791/6940... Peroxi_SPY link: spirochrome.com/products/?_c... 🧪

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Spirochrome @spirochrome.com · 23/03/2026

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Spirochrome @spirochrome.com · 18/03/2026

🤩 Another amazing example of U-ExM actin staining with HAK-actin by @dudinlab.bsky.social et. al.

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Spirochrome @spirochrome.com · 16/03/2026

An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™. These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green). Movie from preprint www.biorxiv.org/content/10.6... 🧪

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Spirochrome @spirochrome.com · 16/03/2026

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Spirochrome @spirochrome.com · 10/03/2026

🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁 Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT) Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology

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Spirochrome @spirochrome.com · 10/03/2026

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Spirochrome @spirochrome.com · 03/03/2026

🟢FastAct™ goes green! 🟢🔬 We launch SPY488-FastAct™ a green channel live cell actin probe SPY488-FastAct allows imaging F-actin in live and fixed cells Compatible with SPY555 and SPY650 for easy 3 color imaging. spirochrome.com/product/spy4... available at our distributors in a couple of days. 🧪

Widefield microscope image of HeLa cells stained with SPY488-FastAct

Widefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)

Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)

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Spirochrome @spirochrome.com · 03/03/2026

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Gerlich Lab @gerlichlab.bsky.social · 25/02/2026

1/New preprint just dropped! 🔥🔥 We investigate how the genome is destroyed in apoptotic cells in a way that prevents DNA fragments from spreading beyond the dying cell 🧬⚰ Done here at @imbavienna.bsky.social & in the super @rcollepardo.bsky.social & Rosen labs: www.biorxiv.org/content/10.6...

biorxiv.org

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Spirochrome @spirochrome.com · 27/02/2026

Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday Easy to use: just dilute the probe in cell culture medium and image 30 min later! 🔗 spirochrome.com/product/spy6... 🙏 Big thanks to @lazaresaladin.bsky.social for the images. 🧪

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Spirochrome @spirochrome.com · 27/02/2026

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Spirochrome @spirochrome.com · 27/02/2026

An amazing preprint by @gerlichlab.bsky.social on the fate of DNA during apoptosis. Definitely to add on your reading list! Our SPY650-DNA probe was heavily used to visualize DNA throughout the manuscript. Congratulations to the team!

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Joey Tidei @j-today.bsky.social · 24/02/2026

SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info!

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Spirochrome @spirochrome.com · 25/02/2026

Another fiery 💥 probes combination by @j-today.bsky.social, this time with SPY555-Tubulin and SPY650-FastAct_X. 🤩

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Spirochrome @spirochrome.com · 23/02/2026

ICYMI SPY555-FastAct_X and SiR-tubulin in action here! 🤯 16h imaging of neurons 💥 Thank you @j-today.bsky.social for posting this gem!

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epithelial mechanics fan club @epimechfc.bsky.social · 08/02/2026

Hello epithelial mechanics fans!! I’m Juanma @juanmagararc.bsky.social 👋 I work on cell mechanics (see celldynamicslab.com) and use Flipper-TR FLIM to probe membrane biophysics in cells. Join me on this tour about Flipper: what it measures, strengths, advice, cool case studies, and cute drawings!

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Kim Gaudin @gaudinkim.bsky.social · 20/01/2026

📐 Visual communication for @spirochrome.com Design for the launch of HAK-actin™, a new fluorescent probe for Expansion Microscopy. Project included a clear usage schematic, flyer, and folded leaflet, with a minimalist, efficient style aligned with Spirochrome’s visual identity.

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Spirochrome @spirochrome.com · 08/01/2026

Are you staining DNA in your ExM samples? We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol. You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies. spirochrome.com/products/?_c...

The images shows an array of 6 Expansion microscopy images of cell nuclei (three columns and 2 rows). each image was taken with a different SPY-DNA probe to stain the nuclear DNA and is pseudocolored to the fluorescence wavelength of the SPY-DNA probe. Trom top left to bottom right: SPY505-DNA (green); SPY555-DNA (magenta); SPY595-DNA (yellow); SPY620-DNA (orange); SPY650-DNA (red); SPY700-DNA (darker red). a white scale bar of 50 micrometers is given for each image to get an impression of the scale of each nuclei.

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Spirochrome @spirochrome.com · 05/01/2026

Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well! Peroxi_SPY can thus be combined with immunofluorescence labeling. A new article provides a detailed protocol: app.jove.com/t/69405/pero... Products link: spirochrome.com/products/?_c...

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Spirochrome @spirochrome.com · 05/01/2026

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Luc Reym⌬nd @lreymond.bsky.social · 05/01/2026

📄New article! Fixed cell Peroxisome imaging is now possible with Peroxi_SPY probes In a new JoVE article Triana Amen, @mschuhmacher.bsky.social and Einat Zalckvar robustly show that Peroxi_SPY stained cells can be fixed and stained by IF! Detailed protocol in the article link (first reply below⬇️)

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Spirochrome @spirochrome.com · 16/12/2025

A new article by @zhixingchen2.bsky.social shows that the far red mitochondrial probe PKmito Deep Red is a robust mitochondrial membrane potential probe when imaged by FLIM. www.nature.com/articles/s41... PKmito Deep Red is exclusively available at Spirochrome: spirochrome.com/product/pkmi...

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Luc Reym⌬nd @lreymond.bsky.social · 16/12/2025

New paper from @zhixingchen2.bsky.social's lab! It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime! www.nature.com/articles/s41...

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Spirochrome @spirochrome.com · 28/11/2025

The unique mechanosensitive probe Flipper-TR is at the core of an amazing article by @juanmagararc.bsky.social and @rouxlab.bsky.social team of membrane tension experts. Congratulations! There is a great 🧵 explaining the key findings how and why cells build membrane tension gradients ⬇️

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Juanma García-Arcos @juanmagararc.bsky.social · 27/11/2025

New paper from the @rouxlab.bsky.social on Nature Communications! We study how membrane tension is spatially organized in cells. Using the mechanosensitive probe Flipper-TR to visualize tension across the plasma membrane of adherent cells and to dissect the conditions needed for a gradient to happen

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Spirochrome @spirochrome.com · 24/11/2025

A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct www.science.org/doi/full/10.... The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects

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Spirochrome @spirochrome.com · 24/11/2025

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Luc Reym⌬nd @lreymond.bsky.social · 19/11/2025

Cool, the HAK-actin preprint was showcased by @prelights.bsky.social A good reason to read it if you haven't yet 😜

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Omaya Dudin 𓂆 ¦🍉¦🦠¦🔬¦ @dudinlab.bsky.social · 19/11/2025

Quite a busy day on #Bluesky ! Been a while since we have been #preLight -ed ! Discover HAK-Actin if you haven't already ;)

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Spirochrome @spirochrome.com · 20/11/2025

Thank you @prelights.bsky.social for highlighting HAK-actin! HAK-actin is a great tool for ExM actin staining. spirochrome.com/product/hak-...

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Claire Brown @clairebrown514.bsky.social · 18/11/2025

Klein Scientific telling us about the products they distribute including @spirochrome.com products. @globalbioimaging.bsky.social #GBI_AU_2025

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Spirochrome @spirochrome.com · 04/11/2025

👉🦠Interested in bacterial membrane tension? Our membrane tension probe Flipper-TR is a top tool for the job! A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension. 🔗 doi.org/10.1073/pnas... 🧪

doi.org

Exploring bacteria–surface interactions with a fluorescent membrane tension probe | PNAS

Understanding how bacteria interact with surfaces is critical for advancing applications in biofilm and biofouling prevention, biomaterial developm...

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Spirochrome @spirochrome.com · 29/10/2025

Find out how SPY650-FastAct_X can help your research here: spirochrome.com/product/spy6...

A group of live HeLa cells stained with SPY650-FastAct_X and imaged by STED microscopy. The image was kindly provided by Prof. Till Stephan.

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