A coordinated sequence of RNA helicases and associated factors dismantles normal and defective spliceosome complexes to enable intron degradation and ensure accurate gene expression.

Acanthamoeba castellanii, a pathogenic amoeba, belongs to an evolutionary branch distinctly removed from humans and fungi. Cryo-EM of the organism’s ATP synthase enzyme using raw mitochondrial lysates...

In eukaryotes, ribosome biogenesis initiates within the nucleolus, a hallmark multilayered compartment of the nucleus. Structures of pre-ribosomes have been characterized ex situ, but their assembly p...

The ciliary transition zone (TZ) regulates ciliary proteome composition, yet its molecular architecture, protein content, and contribution to motile ciliopathies remain poorly defined. We applied in situ cryo-electron tomography and subtomogram averaging ...

Fluorescent proteins and small-molecule dyes offer complementary advantages for biological imaging: proteins are amenable to genetic tagging, whereas dyes provide superior brightness and photostabilit...

The extent to which crystal packing constrains protein dynamics is hard to decipher. Now a combination of NMR, crystallography and molecular dynamics simulations show that intermolecular contacts can ...

In this issue of Cell Host & Microbe, Osterman et al. discover aRES, a new family of bacterial immune proteins that deplete cellular NAD+, generating cleavage products that cannot be utilized by canon...

Phase plates can in principle overcome the poor image contrast in electron cryo–microscopy (cryo-EM) and the resulting limits on the structural reconstruction of small proteins. However, previous desi...

Zhang et al. report cryo-EM structures of AGO2-siRNA complexes capturing RISC assembly intermediates. They reveal MID-PIWI-mediated duplex loading, α14-guided end sensing, and stepwise passenger peeli...

The mitochondrial oxidative phosphorylation system powering most aerobic organisms is typically depicted as two spatially segregated machineries(1): electron transport chain complexes I-IV (ETC CI-CIV

Data from cryogenic electron microscopy combined with hydrogen–deuterium exchange mass spectrometry inform a mechanism for cold-evoked activation of the TRPM8 channel, providing a structural and ...

Can you find the Euler angles of a cryo-EM projection?

To maintain genome stability, the specialised reverse transcriptase telomerase processively adds telomeric DNA repeats to chromosome ends. Here the authors reported structures of human telomerase at t...

In mice, a SPOCD1–TPR-dependent ‘nowhere-to-hide’ mechanism is required for complete non-stochastic piRNA-directed LINE1 DNA methylation by preventing transposons from escaping surveillance within heterochromatin.