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John Rubinstein

@johnrubinstein.bsky.social
2.3K followers 556 following 256 posts

CryoEM, Bioenergetics, V-ATPases (especially in synaptic vesicles and lysosomes), OxPhos (especially in mycobacteria as a TB drug target) Scientist, The Hospital for Sick Children Professor, U of Toronto 👨‍👩‍👧🔬🚲 rubinsteinlab.org COI: structura.bio

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Reposted by John Rubinstein
Matthew Freeman @mjafreeman.bsky.social · 16h
Yet another opportunity @dunnschool.bsky.social! We are looking for an Associate Professor If you have exciting research plans, @dunnschool.bsky.social is a great place: excellent science (and lovely people, obvs) www.path.ox.ac.uk/vacancy/asso...
path.ox.ac.uk
Associate Professorship of Cancer Biology - Sir William Dunn School of Pathology in association with the Queen’s College. - Dunn School
We are seeking to appoint an Associate Professor working in the area of the cellular and/or molecular biology of cancer, to be based in Oxford University’s Sir William Dunn School of Pathology. The ro...
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John Rubinstein @johnrubinstein.bsky.social · 30/09/2026
A new PhD is formed. Congratulations @zestytoast.bsky.social on a spectacular defense! And thanks to Christina Stallings for serving as external examiner (and guest speaker)
Yingke opens door to be greeted by the lab after her defense
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Cezar Khursigara @cmkhursigara.bsky.social · 30/09/2026
Molecular & Cellular Biology at the University of Guelph is recruiting an Assistant Professor in Molecular & Cellular Biotechnology through the Canada Impact+ Emerging Leaders program. Please share widely! careers.uoguelph.ca/job/Guelph-A...
careers.uoguelph.ca
Assistant Professor (Canada Impact+ Emerging Leader)
Assistant Professor (Canada Impact+ Emerging Leader)
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Jim Woodgett @jwoodgett.bsky.social · 25/09/2026
This should be the new Tourism Canada advert. Just perfect.
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Structura Biotechnology @structurabio.bsky.social · 24/09/2026
🇨🇦 Today we kicked off our hands-on workshop, “Beyond Consensus Refinements: Advanced Cryo-EM Data Processing with CryoSPARC,” in collaboration with The Hospital for Sick Children @sickkidsto.bsky.social! Excited for two days of diving deeper into advanced #cryoEM data processing with #CryoSPARC. 🔬
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John Rubinstein @johnrubinstein.bsky.social · 24/09/2026
The @structurabio.bsky.social @sickkidsto.bsky.social CryoSPARC workshop is underway!
Instructor Rich Waldo presents slides on advanced topics in cryo-EM image analysis to an attentive class.
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Reposted by John Rubinstein
Structura Biotechnology @structurabio.bsky.social · 23/09/2026
🔎 A classic #cryoEM particle curation conundrum: 2D or 3D classification? "Decoy Classification" using Heterogeneous Refinement in #CryoSPARC curates particles directly in 3D, helping remove junk particles while preserving valuable views. Read more in the CryoSPARC Blog 👉 bit.ly/3SXfwS0
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John Rubinstein @johnrubinstein.bsky.social · 23/09/2026
www.sickkids.ca/en/news/arch...
sickkids.ca
SickKids named world’s best children’s hospital for second year in a row
Back-to-back No. 1 ranking recognizes the backing of staff and a community committed to child health.
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Jim Sun, PhD @sunlab.bsky.social · 09/09/2026
We are looking for a postdoctoral fellow to join the team @ubcmicroimmuno.bsky.social @science.ubc.ca to study TB pathogenesis and host-directed therapy! Please help disseminate amongst your network. ubc.wd10.myworkdayjobs.com/ubcfacultyjo...
ubc.wd10.myworkdayjobs.com
Postdoctoral Research Fellow - Host-Pathogen Interactions & Host-Directed Therapeutics
Academic Job Category Faculty Non Bargaining Job Title Postdoctoral Research Fellow - Host-Pathogen Interactions & Host-Directed Therapeutics Department Research I Sun I Department of Microbiology...
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Trevor A. Branch @trevorabranch.bsky.social · 09/09/2026
This is seismic news for fisheries scientists. Northern cod is *the* iconic symbol of total fisheries collapse due to overfishing (and other factors) *and* the iconic symbol of a complete failure to recover for decades afterwards. This is like announcing the complete recovery of bison on land.
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Roland Pierik @roleo.eurosky.social · 08/09/2026
The number of cases of cervical cancer reported in Scotland among women given the HPV vaccine at 12-13 years of age is now zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. Zero. www.bbc.com/news/article...
bbc.com
No cervical cancer cases in fully HPV-vaccinated women in Scotland
A study published by Public Health Scotland finds that the HPV vaccine is "highly effective".
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The Royal Society @royalsociety.org · 27/08/2026
Professor Bonnie Bassler is awarded the Copley Medal for her discovery of the universality of chemical communication in bacteria and for her idea that interfering with this communication could form the basis of novel anti-bacterials. #RSMedals royalsociety.org/medals-and-prizes/…
A woman with dark hair and a light blue shirt smiles at the camera in front of a bright white background.
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The Royal Society @royalsociety.org · 27/08/2026
Professor Dame Janet M Thornton DBE FMedSci FRS is awarded the Croonian Medal and Lecture 2027 for her pioneering work in protein structural and functional bioinformatics. #RSMedals royalsociety.org/medals-and-prizes/…
A scientist sits at a desk in front of a computer display with complicated graphics on the screen. She is turned slightly and smiling at the camera.
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The Royal Society @royalsociety.org · 28/08/2026
The Francis Crick Medal and Lecture 2027 is awarded to Dr Thi Hoang Duong (Kelly) Nguyen for her ground-breaking discoveries into the molecular mechanisms of telomere maintenance. #RSMedals royalsociety.org/medals-and-prizes/…
A woman in a blue top with a small colourful pattern smiles at the camera in front of a white background.
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The Royal Society @royalsociety.org · 27/08/2026
Professor Lewis E Kay FRS is awarded the Royal Medal (Biological) 2026 for his remarkable contribution to understand and quantify protein dynamics and their relevance to protein function. #RSMedals royalsociety.org/medals-and-prizes/…
A man in a black shirt and glasses smiles at the camera. You can see a computer screen and framed certificates in the background.
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Pol Solé Navais @polsolenavais.bsky.social · 05/09/2026
📣📣📣 The University of Gothenburg (Sweden) is recruiting 25 (yes, 25!) Assistant Professors in a wide range of disciplines (from arts to education to geology to maths), two of these at our Faculty. Happy to talk to anyone interested in knowing more about Sweden, Gothenburg or GU!
gu.se
Gothenburg Global Scholars Initiative
The University of Gothenburg is launching the Global Scholars Initiative – a coordinated international recruitment effort comprising 25 research positions across a broad range of disciplines and facul...
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John Rubinstein @johnrubinstein.bsky.social · 01/09/2026
I met Ada when I was a new PI before her Nobel Prize. She spotted a copy of F.A. Cotton's "Chemical Applications of Group Theory" on my shelf and proceeded to tell me some of the most interesting stories about science and scientists I've ever heard. She was one of a kind. Rest in Peace.
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Esther Verheyen 🇨🇦 @estherverheyen.bsky.social · 28/08/2026
Today 🇨🇦 announced the 64 scholars recruited from top US universities for the Canada Global Impact+ Chairs. They sounds like great scientists. But the enormous level of funding funnelled to this program is jaw-dropping when compared to the pitiful success rates at CIHR.
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Stephanie Carvin @stephaniecarvin.bsky.social · 27/08/2026
Wake up, babe. The Prime Minister just dropped “hydronym”.
The name Lake Ontario comes from the Wendat word Ontari’io, which, appropriately, means “the lake is beautiful, the lake is big”.

The name is more than 400 years old, predating both the Confederation of Canada and the Declaration of Independence of the United States of America.

We know that America is changing. Their trading relationships, their foreign policies, their national monuments, their hydronyms. 

Canadians also know that naming reality means calling it Lake Ontario – then, now and always.
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Kelly Nguyen @kellythd-nguyen.bsky.social · 27/08/2026
Such an honor to receive the Francis Crick Medal from the Royal Society. As I always say, I am just very lucky to have worked with amazing people. Big shoutout to all past and present lab members, mentors, colleagues and collaborators for continuing inspiring me!
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Deborah Marsh @djmarsh24.bsky.social · 27/08/2026
In April this year at a medical conference (ASCO 2026), results of a drug improving overall survival in patients with #PancreaticCancer received a standing ovation - almost unheard of. Exciting to see the RAS inhibitor #daraxonrasib now approved by the FDA for metastatic pancreatic adenocarcinoma. 🧪
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John Rubinstein @johnrubinstein.bsky.social · 22/08/2026
So many important contributions. The story of the development of abberation-corrected electron microscopy, in which Prof. Rose player a central role, is one of the most remarkable in science. Arguably should have been awarded a Nobel Prize.
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Lars-Anders Carlson @lacarlson.bsky.social · 22/08/2026
Harald Rose, the giant of electron microscopy theory, passed away this summer aged 91. Thankful to his contributions to our field! Ruhe in Frieden, Prof. Rose. en.wikipedia.org/wiki/Harald_...
en.wikipedia.org
Harald Rose - Wikipedia
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The Associated Press @apnews.com · 22/08/2026
BREAKING: The U.S. is imposing 50% tariffs on $20 billion worth of Canadian products after last-ditch negotiations failed. Canada promises to match the tax.
bit.ly
US is set to impose 50% tariffs on $20 billion worth of Canadian products
The United States is set to impose 50% tariffs on $20 billion worth of Canadian products early Saturday after last-ditch negotiations failed to resolve the latest strain in already tense relations between the historic allies.
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Edward Marcotte @edwardmarcotte.bsky.social · 19/08/2026
Exciting news for personalized mRNA cancer vaccines, especially if the phase 3 effect size matches the phase 2 trial (49% reduced death or recurrence), although we have to wait for the official numbers arstechnica.com/health/2026/...
arstechnica.com
mRNA cancer vaccine succeeded in Phase 3 melanoma trial, Moderna and Merck say
The vaccine reportedly thwarted the cancer's return and spread.
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Rhys Grinter @rhyswg.bsky.social · 18/08/2026
Out now in @natmicrobiol.nature.com: Bacillus subtilis builds protein filaments that act as a pseudomembrane, pulling quinone out of the bilayer and carrying it into the cytosol for reduction by NADH dehydrogenase, well away from the membrane itself. doi.org/10.1038/s415... 1/5
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Structura Biotechnology @structurabio.bsky.social · 17/08/2026
🚀 Preprint alert: cFAR and Relative Signal: Diagnosing Preferred Orientation in Single-Particle #CryoEM Explore how cFAR, tFAR, and Relative Signal provide complementary diagnostics for preferred orientation, tested across 14 #EMPIAR datasets and compared with established metrics 👉 bit.ly/4wBY1Ev
cFAR and Relative Signal: Diagnosing Preferred Orientation in Single-Particle CryoEM
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cryoEM papers @cryoempapers.bsky.social · 16/08/2026
cFAR and Relative Signal: Diagnosing Preferred Orientation in Single-Particle Cryo-EM www.biorxiv.org/content/10.64898/2026.08.11.744264v1 #cryoEM
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Jamie Blaza @jnb-lab.bsky.social · 10/08/2026
Permanent academic position as a Lecturer in cryo-ET open at York. We are looking for someone to lead a research program making the most of the stunning advances occurring in cryo-ET. It's a research and teaching post but with a focus on research in the early years. jobs.york.ac.uk/vacancy/lect...
jobs.york.ac.uk
Jobs - The University of York
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Harmit Singh Malik @harmitmalik.bsky.social · 06/08/2026
Come be our colleague. I have been in this Division for 27 years (4 as a postdoc and 23 as a PI). It is an amazing place to work, collaborate, and be inspired. If you are applying to be an Assistant or Associate Professor this Fall, don't miss this deadline.
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Bui Lab @McGill @builab.bsky.social · 04/08/2026
Introducing CsMT: A new CryoSPARC workflow for high-resolution microtubule reconstruction based on novel PF-pair classification! You can reconstruct MTs entirely in CryoSPARC—zero custom scripts needed. Inspired & built through a fantastic collaboration with the Cianfrocco lab! tinyurl.com/kdpm86tw
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Harmit Singh Malik @harmitmalik.bsky.social · 31/07/2026
Wow 🤩 @benoitbruneau.bsky.social Very proud! Big congrats!
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Michael Glickman @michaelglickman.bsky.social · 23/07/2026
Please repost! #microsky🦠 The Glickman Lab at MSK is recruiting! Projects in Mtb pathogenesis, basic biology of mycobacteria, and mycobacterial cancer therapies. See our publications here: tinyurl.com/3f386f4x Send CV and cover letter to: glickmanmycolab@gmail.com
scholar.google.com
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Mark Carney @mark-carney.bsky.social · 24/07/2026
Huge congratulations to Professor Jacob Tsimerman of the University of Toronto on receiving the Fields Medal — one of the highest honours in mathematics. Nearly a century after Canadian mathematician J.C. Fields established this award in Toronto, it comes home.
Screenshot of an article from CBC News. The headline reads: University of Toronto mathematician Jacob Tsimerman wins Fields Medal
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Structura Biotechnology @structurabio.bsky.social · 23/07/2026
🚀 Can automation solve preferred orientation in your #cryoEM datasets? After automating processing across 21 GPCR datasets in #CryoSPARC, we updated the workflow to be even more robust to preferred orientation, achieving improved map quality over manual processing. Read more 👉 bit.ly/3RyOIGG
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Dr. Deanna Santer @drdeannasanter.bsky.social · 14/07/2026
📢 Super excited to promote that our #Immunology department is recruiting a Tier 2 Canada Research Chair in Immunobiology of Chronic Disease!🎉🥳 Please note this is a targeted recruitment with more details via QR code. I am lucky to work here in such a collaborative environment Please share widely
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Structura Biotechnology @structurabio.bsky.social · 14/07/2026
✉️ The latest #CryoSPARC Newsletter is out! Highlights include: 🔹 Sept 2026 workshop hosted with SickKids 🇨🇦 🔹 New cryosparc-tools video 🔹 New case study on discrete heterogeneity 🔹 Small-protein #cryoEM advances 🔹 Dynamic masking guide (v5) Read more here: mailchi.mp/b8fb173dac5c...
Sign up for the newsletter: https://cryosparc.com/#newsletter
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John Rubinstein @johnrubinstein.bsky.social · 06/07/2026
CryoSPARC workshop in Toronto! Beyond consensus refinements: Advanced cryo-EM data processing with CryoSPARC Sept 24-25, 2026 (part of the Canadian Cryo-EM Nexus) Register here: cvent.me/dL1wem Please see details in the link or the image Alt text @structurabio.bsky.social @sickkidsto.bsky.social
Workshop announcement graphic shows a classroom scene with the CryoSPARC logo and workshop title, date, and hosting organizations (SickKids and Structura Biotechnology)

Workshop information:
As part of the Canadian Cryo-EM Nexus, The Hospital for Sick Children (SickKids) and Structura Biotechnology are pleased to announce a two-day CryoSPARC workshop:

Beyond consensus refinements: Advanced cryo-EM data processing with CryoSPARC

at SickKids in Toronto on September 24-25, 2026.

Led by experts from Structura, this workshop offers a unique opportunity for participants who already have a sound understanding of cryo-EM and substantial CryoSPARC experience to deepen their skills and become a key resource for cryo-EM image analysis within their institutions.

There is $115 (CAD) charge to partially defray some of the costs of running the workshop, which is sponsored by SickKids and Structura. Attendees will need to bring their own laptop computer and arrange for travel and accommodation.
Please apply before July 24, 2026 using the following link:
https://cvent.me/dL1wem
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John Rubinstein @johnrubinstein.bsky.social · 06/07/2026
CryoSPARC workshop in Toronto! Beyond consensus refinements: Advanced cryo-EM data processing with CryoSPARC Sept 24-25, 2026 (part of the Canadian Cryo-EM Nexus) Register here: cvent.me/dL1wem Please see details in the link or the image Alt text @structurabio.bsky.social @sickkidsto.bsky.social
Workshop announcement graphic shows a classroom scene with the CryoSPARC logo and workshop title, date, and hosting organizations (SickKids and Structura Biotechnology)

Workshop information:
As part of the Canadian Cryo-EM Nexus, The Hospital for Sick Children (SickKids) and Structura Biotechnology are pleased to announce a two-day CryoSPARC workshop:

Beyond consensus refinements: Advanced cryo-EM data processing with CryoSPARC

at SickKids in Toronto on September 24-25, 2026.

Led by experts from Structura, this workshop offers a unique opportunity for participants who already have a sound understanding of cryo-EM and substantial CryoSPARC experience to deepen their skills and become a key resource for cryo-EM image analysis within their institutions.

There is $115 (CAD) charge to partially defray some of the costs of running the workshop, which is sponsored by SickKids and Structura. Attendees will need to bring their own laptop computer and arrange for travel and accommodation.
Please apply before July 24, 2026 using the following link:
https://cvent.me/dL1wem
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John Rubinstein @johnrubinstein.bsky.social · 04/07/2026
New preprint! Karin Schumacher's postdoc Nadja used our SidK approach to purify Arabidopsis V-ATPase & Mariia Khamina determined its structure. The structure shows unique plant V-ATPase features and that TLDc proteins (here OXR5) bind V-ATPase in all eukaryotes. 🌱 tinyurl.com/mw9xaszk
Overall structure of Arabidopsis V-ATPase. (A) SDS-PAGE of purified V-ATPase. (B) Composite map of Arabidopsis V-ATPase in rotational state 1. (C) Atomic model of Arabidopsis V-ATPase in rotational state 1. (D) Cross sections of the V1 and VO regions in three rotational states. (E) Conformations of subunit H in V-ATPase structures of Arabidopsis V-ATPase (top) and the citrus fruit (bottom) V-ATPase.V-ATPase from the TGN/EE interacts with the TLDc protein OXR5. (A) Composite map of Arabidopsis V-ATPase in rotational state 1 bound to OXR5 (green density). (B) AlphaFold3 model (left) and domain organisation (right) of OXR5. (C) VHA-a1-mNG is localized at the TGN/EE. OXR5-PmScarlet-I shows partial overlap with VHA-a1-mNG at the TGN/EE. Scale bar, 20 µm. Root tip elongation zone of 5-day-old Arabidopsis seedlings were analyzed by confocal laser scanning microscopy. (D-H) Atomic model of Arabidopsis V-ATPase interactions with OXR5.
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Baptiste Lacoste @blacoste.bsky.social · 04/07/2026
🚨 We are recruiting at the Ottawa Hospital Research Institute, in the Regenerative Medicine Program ! If your are passionate about basic science, and dream to develop your own research program, please apply! #Ottawa #Canada #Recruitment #Scientist #Research Full description at the link below:
uottawa.wd3.myworkdayjobs.com
Tier 2 Canada Research Chair in Vascular Links to Neurodevelopment
Follow us on LinkedIn Posting Reason: New Position Job Type: Contingent Worker Anticipated Duration in Months (for contracts and temporary assignments): Job Family: Academic Leadership and Faculty # o...
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Utrecht Virology Lab @utrechtvirology.bsky.social · 02/07/2026
📢 We're hiring! We are looking for an Assistant or Associate Professor to join our team at Utrecht University. If you're excited about leading an independent research programme in a collaborative #virology community, we'd love to hear from you. Please share! 👇🦠 #AcademicJobs #FacultyPosition
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John Rubinstein @johnrubinstein.bsky.social · 01/07/2026
First Eurovision, next the EU... 🇨🇦🇪🇺 www.cbc.ca/news/enterta...
cbc.ca
Canada will enter the 2027 Eurovision Song Contest | CBC News
CBC/Radio-Canada and the European Broadcasting Union have announced Canada will participate in the 2027 Eurovision Song Contest in Bulgaria.
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RCSB Protein Data Bank @rcsbpdb.bsky.social · 01/07/2026
Thankfully quarantine for MV Hondius cruise passengers just ended without any new cases, but it is always a good time for Molecule of Month to explore how a family of rodent-borne viruses can cause severe illness in humans...
pdb101.rcsb.org
PDB101: Molecule of the Month: Hantavirus
Family of rodent-borne viruses that can cause severe illness in humans
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Chi-Min Ho @cmholab.bsky.social · 30/06/2026
Incredibly proud of Messi Haile @mehsehret.bsky.social, a founding member of the Ho Lab, for her beautiful work uncovering the inner workings of a central piece of the malaria parasite invasion machinery: the malarial moving junction, out today in @cellpress.bsky.social! tinyurl.com/4p3md2eb 🦠❄️🔬
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Mikko Taipale @miketilapia.bsky.social · 30/06/2026
Our evil ORFeome paper is out! In collaboration with @alex-stark.bsky.social, we screened ~4,000 viral proteins and secreted effectors from bacteria and parasites, covering hundreds of diverse pathogens, for phenotypes in human cells.
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Kelly Nguyen @kellythd-nguyen.bsky.social · 30/06/2026
Honored to be elected to EMBO,together with Manu, Julian and ex-benchmate @wojtekgalej.bsky.social. I’m grateful to all past and present Iab members for their amazing work and support from colleagues, mentors and family over the years.
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The Globe and Mail @theglobeandmail.com · 26/06/2026
theglobeandmail.com
Women’s Auxiliary Volunteers at SickKids bring warmth to patients and their families
Members of the WAV, the Toronto hospital’s most well-known volunteer group, have been providing support for 75 years
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Kresten Lindorff-Larsen @lindorfflarsen.bsky.social · 23/06/2026
Come join the new Novo Nordisk Foundation Center for Protein Design as a group leader Cool science in a great scientific environment in wonderful and very family friendly Copenhagen There are two positions that can either be at TT assistant or associate prof level employment.ku.dk/tenure-track...
employment.ku.dk
Tenure-track Assistant Professor or Associate Professor of De novo Protein Design
The Novo Nordisk Foundation Center for Protein Design at the University of Copenhagen seeks to appoint two new group leaders in de novo protein design from 1 February 2027 or as soon as possible thereafter. These positions will be at the Assistant or Associate Professor level and associated with the Department of Biology (Faculty of Science) or the Department of Drug Design and Pharmacology (Faculty of Health and Medical Sciences).
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