In eukaryotes, ribosome biogenesis initiates within the nucleolus, a hallmark multilayered compartment of the nucleus. Structures of pre-ribosomes have been characterized ex situ, but their assembly p...

In a recent article by Haile et al., the atomic-level structure of the protein complex responsible for the invasion of the malaria parasite into red blood cells is described. Here, the key findings and the implications for the development of next-generation antimalarials are reviewed.

The ciliary transition zone (TZ) regulates ciliary proteome composition, yet its molecular architecture, protein content, and contribution to motile ciliopathies remain poorly defined. We applied in situ cryo-electron tomography and subtomogram averaging ...

Cell entry of nonenveloped animal viruses requires translocation of a macromolecular assembly across a cellular membrane. Double-stranded RNA viruses introduce into the target cell an inner capsid par...

“We will be known forever by the tracks we leave.”

Li et al. obtain high-resolution, native structures revealing that cytoplasmic lattices in mammalian eggs organize and store degradative and cytoskeletal complexes for early embryonic development.

Isolation and high-resolution structure of the moving junction protein complex from invading malaria parasites reveals an intricate molecular machine that bridges the host-pathogen interface and informs the de novo design of a small protein binder that inhibits parasite entry into host cells.

Improved fractionation strategies can identify antibiotics with previously unseen scaffolds and mechanisms, exemplified by manikomycin from Streptomyces rimosus, which acts by targeting the E-site of ...

Author summary Parasites in the phylum Apicomplexa maintain their intracellular lifestyle using specialized organelles that mediate the lytic cycle of host cell invasion, intracellular replication, an...

Highlighted Article: MyoF regulates actin organization in T. gondii by assembling into an oligomeric complex that contains multiple motor domains.

AP3 generates clathrin-free carriers on endosomes by organizing via ARF1 dimers into elongated membrane-deforming arrays.

Cryo-electron tomography (cryo-ET) is emerging as a transformative tool for structural biology. Unlike methods based on purified molecules, cryo-ET en…

Using cryo-electron tomography, the authors reveal how plasmodesmata (plant cytoplasmic bridges) are gated by cell wall remodelling and how helical protein assemblies scaffold internal membranes, with...

Eukaryotic cytochrome (cyt) c is a highly conserved mitochondrial protein central to cellular respiration, featuring a covalently attached hexacoordinate heme whose redox potential is tuned by axial H...

The kinase ZAK is activated at collided ribosomes to mediate the ribotoxic stress response.