Abstract. To effectuate RNA interference (RNAi), Argonaute (AGO) proteins recognize and load RNA guide strands by their requisite 5′-phosphate (5′-P). Howe

DICER1 is often mutated in cancer, including in DICER1-related tumour predisposition. Here, the authors show that cancer-associated DICER1 mutations shift microRNA processing and reactivate HERVH endo...

Background Pathogenic variants in AGO2, encoding a central component of the RNA-induced silencing complex (RISC), cause the neurodevelopmental disorder Lessel-Kreienkamp syndrome (LESKRES). The varian...

Small interfering RNAs (siRNAs) are widely used to repress gene expression. For human Argonaute2 (AGO2) to cleave target RNA, siRNAs must be almost...

Very short RNAs play critical roles in modern biology, and are thought to have been crucial for genome replication during the origin of life. Next-generation sequencing is an essential tool for charac...

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LIMD1 helps AGO2 silence microRNA targets more effectively, revealing an additional layer of control beyond RNA sequence alone.

Human Argonaute2 (AGO2) binds siRNAs to target complementary mRNAs. Although guided–target complementary is required for slicing, there is a wide range of slicing efficacy. A study now shows the preci...

PIWI proteins are members of the Argonaute family and together with piRNAs protect metazoan germlines from transposons. PIWI proteins adopt a bi-lobed architecture with a central RNA-binding channel. ...

Author summary In order to make spermatozoa, germ cells undergo the meiotic specialized cell division, during which the sex chromosomes are temporarily shut down in a process called Meiotic Sex Chromo...

Human Argonaute 2 requires distortion of the paired small interfering RNA (siRNA)–target RNA duplex, not just base pairing, to cleave targets. Structures show how RNA shape and key residues enable cat...

Immune checkpoint inhibitors are used to treat advanced non-small cell lung cancer to activate an antitumor immune response, however they fail in many patients due to resistance. This study shows that...

This study shows that yolk, a conserved vehicle for nutrient provisioning to developing eggs, also transports regulatory small RNAs, such as microRNAs, from somatic tissues to the germline and embryos...

Gregory J. Hannon passed away in April 2026 at the age of 61. A towering figure in modern molecular biology, Greg influenced remarkably diverse areas of science. His work reshaped our understanding of...

Zhang et al. report cryo-EM structures of AGO2-siRNA complexes capturing RISC assembly intermediates. They reveal MID-PIWI-mediated duplex loading, α14-guided end sensing, and stepwise passenger peeli...

Structures of the AGO maturation complex reveal how chaperones and an RNA duplex drive assembly of the RNA-induced silencing complex.

Highly conserved mRNA 3′ UTRs act as co-translational chaperones for intrinsically disordered regions (IDRs), preventing inter-domain misfolding and enabling biogenesis of fully active proteins.

MicroRNAs control gene expression, but their direct RNA targets and turnover mechanisms remain difficult to study. Here, the authors present CLASHub, an integrated database and analysis platform that ...

Target-directed microRNA degradation is driven by the atypical ZSWIM8–CUL3 E3 ubiquitin ligase that uses a two-RNA-factor authentication mechanism to specifically recognize AGO–miRNA–trigger...

Insights into microRNA processing guide the design of an RNA medicine strategy that enables precise multitargeting against cancer.

Planarian extracellular vesicles carry small RNAs that mediate systemic gene silencing and coordinate regeneration.

Postdoktortjänst inom Excellenscentrat Livets Kemiska Mekanismer vid Uppsala universitet, Institutionen för kemi - LV, Uppsala universitet

ARGONAUTE (AGO) proteins are a highly conserved family of RNA-binding proteins that play central roles in gene regulation and developmental process...

A biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields

MicroRNAs guide Argonaute proteins to repress gene expression. Here, the authors define the binding rules for five Drosophila miRNAs, showing a narrow preference for canonical seed sites and identifyi...

Abstract. MicroRNAs (miRNAs) are central regulators of gene expression, yet how cells choose between the two strands (5p or 3p) of a miRNA duplex during bi

Raad et al. describe the cryo-EM structure of mouse MIWI in complex with pachytene piRNAs to reveal a larger RNA-binding channel. They also identify a putative tryptophan-binding pocket on the PIWI do...

Abstract. Short interfering RNAs (siRNAs) represent a novel class of therapeutic modalities, where, in the context of complex chemical modification pattern

Developers have married gene-modulating oligonucleotides with the targeted precision of antibodies, and the first filings using such conjugates in Duchenne muscular dystrophy are imminent.

Mutations in Argonaute proteins (AGOs) cause Argonaute syndrome, a neurodevelopmental disorder (NDD), yet the underlying molecular mechanism remain...

A biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields