Neurons rely on localized protein synthesis to rapidly adapt synaptic function to activity, yet how dendritic translation regulates mitochondrial remodeling during synaptic plasticity remains poorly u...

Here, we combined ribosome profiling under conditions of mTOR inhibition and m⁶A mapping to identify distinct subsets of mRNAs that differ in their sensitivity to the suppression of cap-dependent initiation.

Mitochondrial DNA (mtDNA) encodes core subunits of the oxidative phosphorylation machinery, and its expression is spatially organized, with the genome packaged into nucleoids around which transcriptio...

University of California, San Diego is hiring. Apply now!

Mitochondria perform diverse metabolic and signaling functions, yet how these activities are spatially organized within the mitochondrial network of cells remains poorly understood. Organelle contact ...

Lysosomal dysfunction is a well-recognized feature of aging. Here, we used a suite of tools for rapid lysosomal isolation to construct a multitissue atlas of the metabolite changes lysosomes undergo d...

Abstract. Efficient purification of specific RNAs from lysates remains a major challenge. Existing methods, such as MS2 tagging or oligonucleotide hybridiz

Steady-state RNA abundance measurements mask the synthesis and decay rates that shape gene expression. Analog intrinsic recoding sequencing (AIR-seq) repurposes the base-pairing properties of N4-hydro...

Chang et al. show that co-translational protein misfolding feeds back to ribosomes, slowing translation elongation and triggering mRNA decay. The ribosome-associated chaperone Zuo1 mediates this respo...

Mitochondrial function depends on the maintenance of its genome, and disruptions in copy number and distribution are hallmarks of mitochondrial disorders. Mitochondrial DNA (mtDNA) replication is spat...

The Princeton Pulse Podcast shares breakthroughs in genetics, molecular biology, and genomics that are helping researchers understand how we can live longer and healthier.

UC San Diego received a $5 million NIH renewal for its FIRST program, supporting faculty recruitment, mentorship and career development for 12 rising biomedical researchers.

Cells maintain membrane function by actively balancing the molecular shape of their lipids. Milshteyn et al. find that, when subjected to high hydrostatic pressure, yeast and human cells, but not bact...

RNA-binding proteins (RBPs) orchestrate post-transcriptional processes, including splicing, cleavage and polyadenylation, and translation. Our updated…

In eukaryotic cells, mitochondria form networks that range from highly fused interconnected structures to fragmented populations of individual orga...

Xie et al. show that mitochondrial translation elongation rate influences OXPHOS biogenesis. In the filamentous fungus Neurospora crassa, MRM1 slows elongation speed independently of its methyltransferase activity by simultaneously binding mitoribosomes and mRNAs, thereby coordinating synthesis with folding and membrane insertion of mitochondrially encoded OXPHOS subunits.

Garcia et al. identify a non-canonical function of the splicing factor U2AF1 in translation and localization of nuclear-encoded mitochondrial RNAs. An oncogenic missense mutation leads to pervasive ch...

The distribution of mitochondrial DNA–containing nucleoids is essential for mitochondrial function and genome inheritance; however, no known mechanisms can explain nucleoid segregation or their regula...

Nature Protocols - This Protocol describes the transcriptome-wide labeling of RNAs in a particular subcellular compartment using proximity biotinylation by localized APEX2 enzyme and subsequent...

Mitochondria have a plethora of roles in cells, many of which are related to dynamic changes in their size, shape, and intracellular location. Mitochondrial morphology is commonly assessed by microsco...

Mitochondrial research and genome maintenance at Ludwig-Maximilians-University.

Geisterfer, Jalihal et al. show spatially distinct effects of Whi3 condensates on target translation in Ashbya syncytia. In vitro, translation is enriched at condensate–solute interfaces but repressed...