3-Phosphoinositides are essential cellular lipids regulating health and disease. Among them, phosphatidylinositol-3,5-bisphosphate [PI(3,5)P2] remains the least understood. Using a newly developed rat...

Lennartz et al. evaluate lipid enrichment in clathrin-coated pits and observe that the differential lipid partitioning into clathrin-coated pits is largely driven by the lipid asymmetry of the plasma ...

Nanorulers serve as essential calibration standards to validate optical super-resolution microscopy down to the Ångström scale. Complementary DNA-, protein-, and virus-based scaffolds provide unique ...

Short, DNA-based fluorophore labels that transiently and repeatedly bind to DNA-labeled antibodies enable super-resolution STED imaging. Compared to covalent labels, dynamic labels constantly exchang...

Radulovic et al. show that phosphatidylinositol 3-phosphate (PI3P), produced by the phosphatidylinositol 3-kinase PIK3C3, is formed on ER domains after lysosome damage. ER domain PI3P helps to recruit...

Lysosomes degrade extracellular and intracellular materials targeted for disposal. Lysosomes display an acidic milieu established by the V-ATPase that promotes this hydrolytic activity and powers memb...

Slivka et al. track dynein’s microtubule-binding domain with minimal fluorescence photon flux, revealing transient backward ‘dips’ before steps. These dips represent microtubule release upon ATP bindi...

COLLEGE STATION, TX — Sources across the Varma laboratory at Texas A&M University confirmed Monday that research technician Marcus Cole, 28, who can accurately pipette an entire 384-well plate while…

Cell entry of nonenveloped animal viruses requires translocation of a macromolecular assembly across a cellular membrane. Double-stranded RNA viruses introduce into the target cell an inner capsid par...

Neutrophils maintain migration speed in confined capillaries through adaptive actomyosin remodeling.

The detailed study of membrane contact sites is challenging owing to their small size and highly dynamic nature. This Review highlights advanced imaging methods and available biosensors that can be us...

Increased activity of leucine-rich repeat kinase 2 (LRRK2) confers Parkinson's disease risk. LRRK2 dynamically localizes to lysosomal membranes in response to various stresses, yet the mechanisms by w...

Popular vendors’ catalogs feature more than 18,000 manipulated images

Coronins are evolutionarily conserved proteins involved in many biological processes, but exactly how coronin proteins exert their function is unclear. This study shows that peptide or fluorescent pro...

MINFLUX nanoscopy reveals subcellular distribution and conformational diversity of PIEZO1 channels in intact cells.

Eilts and colleagues combine a double-homogenisation expansion microscopy approach with dSTORM, revealing molecular details of endogenous protein complexes, including microtubules, clathrin lattices a...

In cells, ESCRT-III is unique in mediating fission of membrane necks from inside, a process called reverse-topology fission. Yet, in vitro, the complex primarily assembles outside membrane necks and m...

SAN DIEGO, CA—Citing time constraints, Dr.

Oatman et al. introduce PyCLM, a Python suite for closed-loop optogenetic experiments combining real-time biosensor measurement and segmentation of thousands of cells. The method is applied to epithel...