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Séamus Holden 🔬🦠🧫

@seamusholden.bsky.social
2.9K followers 1.3K following 118 posts

Professor of Microbial Biophysics, University of Warwick. Interested in all things bacterial cell envelope, cell biophysics and super-resolution microscopy. holdenlab.github.io

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Reposted by Séamus Holden 🔬🦠🧫
MRC Laboratory of Molecular Biology @mrclmb.ac.uk · 02/10/2026
Considering a PhD in 2027? Applications for Gates Cambridge Scholarships @gatescambridge.bsky.social are now open for October 2027 PhD entry (deadline: 14 October 2026). Learn more about PhD study at the LMB: mrclmb.ac.uk/careers-and-...
mrclmb.ac.uk
PhD Students | MRC Laboratory of Molecular Biology
PhD Students Budding scientists from all over the world undertake postgraduate research at the LMB. Our students are creative and innovative, driving the su ...
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Reposted by Séamus Holden 🔬🦠🧫
Institut Jacques Monod @ijmonod.bsky.social · 01/10/2026
✍️ The Bonazzi Lab published a new article 📖 @natcomms.nature.com 📃Bacterial growth under confinement requires transcriptional adaptation to resist turgor pressure build-up 🔗 www.nature.com/articles/s41...
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Robin Bisson @robinbisson.bsky.social · 30/09/2026
The Labour Party conference didn’t deliver any surprises on R&D, but it was possible to feel which way the wind is blowing I sat down with Emily Darlington MP to talk about it. As an adviser during the New Labour years, both on science and within the Treasury, she had some important insights
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Ákos T Kovács @evolvedbiofilm.bsky.social · 30/09/2026
The phenotypic landscape of the model firmicute Bacillus subtilis PNAS from Carol A. Gross www.pnas.org/doi/10.1073/...
pnas.org
The phenotypic landscape of the model firmicute Bacillus subtilis | PNAS
Firmicutes are gram-positive bacteria with important roles in human health, disease, and industry. However, more than a quarter of genes in the mod...
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Freya Harrison @friendlymicrobe.bsky.social · 30/09/2026
This will be a brilliant post with a super team #MicroSky
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Mark Peifer (He, him) @peiferlabunc.bsky.social · 28/09/2026
Today's winning Biology All email--in fact this is a contender for best this year
Hi all,

We received a package with no name to GSB 2144 with 80 sheep brains in it. If you are expecting this package, please reach out and we can coordinate the pickup!

Best wishes,
Jenna
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Reposted by Séamus Holden 🔬🦠🧫
Erin Purcell @erinpurcell.bsky.social · 24/09/2026
'We have replaced the danger of mouth-pipetting with the danger of a generation of scientists spending their most productive years writing grants that will not be funded. This is its own form of danger, just slower and less honest than the phenol.' Onion-level satire with a side of 'oooof.'
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Reposted by Séamus Holden 🔬🦠🧫
Steve Russell @steverus.bsky.social · 24/09/2026
We’re recruiting to an assistant/associate professorship in Pathogen Genetics- come and join us. www.cam.ac.uk/jobs/assista...
cam.ac.uk
Assistant/Associate Professor in Pathogen Genetics
Applications are invited for an Assistant or Associate Professorship in Pathogen Genetics to be held in the Department of Genetics. We are searching for a talented individual with a track record of fu...
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 18/09/2026
Intriguing preprint on effects of cyclic di-AMP on Strep pneumo cell division, looking forward to reading properly www.biorxiv.org/content/10.6... I wonder this is related to effect of ci-di-AMP on turgor #microsky
biorxiv.org
Cyclic di-AMP signalling affects cell division and penicillin susceptibility in Streptococcus pneumoniae
Resistance to β-lactam antibiotics in the human pathogen Streptococcus pneumoniae is mainly attributed to the acquisition of mutated versions of pbp2x , pbp2b and pbp1a , encoding functional penicilli...
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 18/09/2026
Really impressing MINFLUX tracking of molecular motors www.nature.com/articles/s41... #bioimaging
nature.com
Characterizing dynamics of productive dynein stepping by MINFLUX - Nature Structural & Molecular Biology
Slivka et al. track dynein’s microtubule-binding domain with minimal fluorescence photon flux, revealing transient backward ‘dips’ before steps. These dips represent microtubule release upon ATP bindi...
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 18/09/2026
Really neat natural language based microscope control software from @jonasries.bsky.social lab www.biorxiv.org/content/10.6... #bioimaging
biorxiv.org
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Reposted by Séamus Holden 🔬🦠🧫
Charlotte Walker @charlottewalker.bsky.social · 28/10/2023
Someone sent me this meme about peer reviewer comments and it is 👌🏻 #PhDSky #AcademicSky
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Cllr Dr Alex Powell @apowelllaw.bsky.social · 15/09/2026
All eyes on Brighton. These actions are hideous. But they also suggest serious issues at the institution, are we about to see the first institution go bust? Without government action, this decimation of our universities will continue to and accelerate. Where is the Prime Minister?
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James Connolly @ruamicro.bsky.social · 14/09/2026
Are you a motivated researcher interested in bacteria-host interactions? We have postdoc position available in my lab to join our Wellcome Trust funded collaborative team. We will study the role and regulation of the enigmatic sphingolipids in E. coli. Please share. www.jobs.ac.uk/job/DSX115/r...
jobs.ac.uk
Research Assistant/ Associate in Bacteria-Host Interactions at Newcastle University
Explore an exciting academic career as a Research Assistant/ Associate in Bacteria-Host Interactions. Don't miss out on other academic jobs. Click to apply and explore more opportunities.
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Reposted by Séamus Holden 🔬🦠🧫
Freya Harrison @friendlymicrobe.bsky.social · 03/09/2026
It's taken 12 years and a lot of work from many fantastic people, but we have published an analysis of how our reconstruction of Bald's eyesalve kills bacteria. This was driven by @tosinorababa.bsky.social & Jess Furner-Pardoe through their PhD work #MicroSky journals.asm.org/doi/10.1128/... [1/n]
Jess in the lab, holding an onion and a garlic bulb and talking; Tosin in the lab holding a petri dish and inspecting it; two glass bottles labelled "eyesalve 33/6/14"
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Christophe 🔬 Leterrier @christlet.bsky.social · 02/09/2026
Really cool! Smaller than GFP and reversibly binding last-generation rhodamine dyes. De novo pan-rhodamine binders for fluorescence microscopy from mammalian cells to extremophiles www.cell.com/cell/fulltex... Congrats @bohuang.bsky.social @rhodamine110.bsky.social @mullinslab.bsky.social et al
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Reese Richardson @reeserichardson.bsky.social · 25/08/2026
A massive update: At least ~15 companies~ are selling scientists antibodies using faked validation data. We've documented 18,000+ manipulated images on 17,000+ products sold by leading laboratory suppliers including Thermo Fisher, Abcam, Santa Cruz Biotechnology, Millipore Sigma and Bio-Techne. 1/🧵
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EMBO @embo.org · 24/08/2026
Sign up now for the EMBO Workshop "Bacterial and archaeal #CellDivision: Diverse mechanisms and models" in Barga (Lucca), Italy, 20–23 April 2027. Deadline: 6 January 2027 meetings.embo.org/event/27-cell-div… #EMBObacDiv27 #genetics #biochemistry #StructuralBiology #EMBOevents 🧪
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Susan Schlimpert @s-lab.bsky.social · 25/08/2026
It is happening, and registration is open for the EMBO Workshop: Bacterial and archaeal cell division: diverse mechanisms and models. 20–23 April 2027 | Barga, Italy. Come and join us @awehenkel.bsky.social, @seamusholden.bsky.social, @s-lab.bsky.social ! Deadline: Jan 6, 2027. #Bacdiv27
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BWJones @bwjones.bsky.social · 19/08/2026
An excellent thread by @doctorow.pluralistic.net on defining AI simply as a technology that is currently in a massive overhype cycle. But this overhype is doing real damage to our lives, environment, and sense of society. The sooner we contextualize it as another technology, we can regain sanity.
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Dave Baltrus 🦦 @surtlab.bsky.social · 19/08/2026
+1 for my own inherent biased viewpoint actually aligning with data
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 22/07/2026
Jobs: My fantastic membrane biologist colleague Satyajit Mayor (warwick.ac.uk/fac/sci/med/...) has a really exciting single molecule #biophysics PDRA position currently advertised based at @warwickmed.bsky.social warwick-careers.tal.net/vx/lang-en-G...
warwick-careers.tal.net
Research Fellow in Membrane Biophysics (111600-0526) - University of Warwick
Title: Research Fellow in Membrane Biophysics (111600-0526). Application Deadline: . Position Type: Fixed Term
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Reposted by Séamus Holden 🔬🦠🧫
James Connolly @ruamicro.bsky.social · 10/07/2026
Fantastic opportunity to join a brilliant lab and wider collaborative team!
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Graham Dellaire @docdellaire.bsky.social · 15/06/2026
🔥Kind of a big advance in CryoEM - www.science.org/doi/10.1126/...
science.org
Laser phase plate improves structure determination of small proteins by cryo-EM
Phase plates can in principle overcome the poor image contrast in electron cryo–microscopy (cryo-EM) and the resulting limits on the structural reconstruction of small proteins. However, previous desi...
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Reposted by Séamus Holden 🔬🦠🧫
Freya Harrison @friendlymicrobe.bsky.social · 15/06/2026
Hey #MicroSky, can anyone tell me offhand whether Klebsiella pneumoniae ATCC13883 has rough or smooth LPS please? My searches are failing. Thank you :)
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Reposted by Séamus Holden 🔬🦠🧫
Christophe 🔬 Leterrier @christlet.bsky.social · 15/06/2026
Banker-type hippocampal neuron culture world championship
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 01/06/2026
Update on GeneFrame alternative for bacterial imaging. After discussion with us and others, SunJin lab have how launched a substitute for timelapse imaging in geneframes www.sunjinlab.com/product/ispa... Dimensions are very similar, price is reasonable ($105 for 250) #microsky
sunjinlab.com
iSpacer 0.3mm – SUNJIN LAB
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 21/05/2026
Still some time to apply for this faculty position at Warwick!
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Prof Paula Salgado @pssalgado.bsky.social · 12/05/2026
Just over a week left to apply for this #PhD #studentship on S-layers and #AMR! Please share and if you want to know more, get in touch!
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Exciting Instruments @ei-science.bsky.social · 05/05/2026
🤔 Still wondering how smFRET works in practice - and how it complements your current techniques? Our handbook shows when and why to use it alongside existing methods to add dynamic, single-molecule insight. Download: buff.ly/CxuqL1A #smFRET #biophysics #biochemistry
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Prof Paula Salgado @pssalgado.bsky.social · 29/04/2026
🧫 #AMR #PhD open @newcastleuni.bsky.social! How does #Cdifficile protein "armour" help it resist antimicrobials? Join me and @henrikstrahl.bsky.social to find out using: 🔬 #Structuralbiology, #microbiology #microscopy #genetics 📅 Deadline 20 May 2026 #PhD #PhDLife Pls share! (UK home only)
findaphd.com
C. difficile S-layer’s role in antimicrobial resistance at Newcastle University on FindAPhD.com
PhD Project - C. difficile S-layer’s role in antimicrobial resistance at Newcastle University, listed on FindAPhD.com
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 22/04/2026
There's more - we also have a broad call for 6 Assistant Professors in @warwicklifesci.bsky.social www.nature.com/naturecareer...
nature.com
Assistant Professor x6 - Coventry, West Midlands (GB) job with The University of Warwick | 12858563
The School of Life Sciences (SLS) wishes to appoint six excellent and ambitious early career principal investigators
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 22/04/2026
Join us! We are recruiting an Associate/ Assistant Professor in quantitative, systems and engineering biology (QSEB) @warwicklifesci.bsky.social . warwick-careers.tal.net/vx/lang-en-G...
warwick-careers.tal.net
Associate Professor or Assistant Professor in Quantitative, Systems and Engineering Biology (111546-0426) - University of Warwick
Title: Associate Professor or Assistant Professor in Quantitative, Systems and Engineering Biology (111546-0426). Application Deadline: . Position Type: Permanent
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Christophe 🔬 Leterrier @christlet.bsky.social · 22/04/2026
A review on Super-resolution Structured Illumination Microscopy (SIM) to study endocytosis: pubs.acs.org/doi/10.1021/... Featuring our live-cell SIM of proximal axons showing frozen clathrin-coated pits within clearing of the spectrin mesh (doi.org/10.1126/scie...)
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Brian Cook @bcryo.bsky.social · 23/03/2026
1) The Herzik lab is excited to announce our newest preprint in collaboration with the Grotjahn lab! We have developed a surfactant cocktail that seeks to overcome one of the more nefarious problems in #cryoEM, Air-water interface (AWI)-induced damage and preferred orientation (PO).
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 11/03/2026
Neat paper from Schwille lab. They demonstrate constriction of synthetic vesicles using important components of the bacterial cell division machinery, FtsZ and FtsN. They induce strong constriction of Z-rings in vesicles just by adding the cytoplasmic domain of FtsN www.nature.com/articles/s41...
nature.com
Optimizing spatial organization of FtsZ rings for large-scale constriction in synthetic cells - Nature Communications
Spatially regulated membrane constriction is an important milestone in reconstituting minimal cell division. Here the authors engineer a truncated system where the cytosolic domain of FtsN forms large...
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bioRxiv Biophysics @biorxiv-biophys.bsky.social · 11/03/2026
3D-MINSTED nanoscopy and protein tracking in densely labelled living cells www.biorxiv.org/content/10.64898/20…
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Ellie Boardman @ellieboardman.bsky.social · 24/02/2026
Come work with us in Oxford! www.jobs.ac.uk/job/DQL601/p...
jobs.ac.uk
Postdoctoral Research Associates in the Molecular Analysis of Bacterial Outer Membrane Biogenesis at University of Oxford
An opportunity for an academic position as a Postdoctoral Research Associates in the Molecular Analysis of Bacterial Outer Membrane Biogenesis is available, as advertised on jobs.ac.uk. Apply now and ...
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 24/02/2026
There's still time to apply for this PhD position!
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Freya Harrison @friendlymicrobe.bsky.social · 18/02/2026
During her PhD, Jenny found something unexpected: biofilm-deficient P. aeruginosa mutants were just as tolerant to antibiotics as the WT when grown in our cystic fibrosis lung model. But why? #MicroSky 1/n
Four graphs, showing the response of PA14 and an isogenic pel mutant, and PAO1 and an isogenic pel psl double mutant, to meropenem and colistin. There is no difference between the response of mutants and WTs.
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Henrik Strahl @henrikstrahl.bsky.social · 15/02/2026
This is a nice one! Congratulations @carballido-lab.bsky.social et al! Nice to see you agree that this might lead to local enrichment of cell wall precursor lipids. Not easy to prove but I still think its an attractive model (to ensure high CW synthesis given low lipid II levels).
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Fusion Conferences @fusionconf.bsky.social · 10/02/2026
The demand for #ProkaryoticCellBio so far has been crazy! We received a record number of abstract & grant submissions, to which the conference chairs are busy reviewing! We predict poster slots & accommodation to begin filling up, don't wait until the deadline to register! More info ➡️ bit.ly/4raYCu
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 09/02/2026
Preprint on the permeability of Gram+ bacterial cell wall from a few months ago. Intriguing findings, elegant methodology. Cell wall is largely impermeable to molecule sizes of mNeonGreen (27kDa) and above. How do larger secreted proteins get through? www.biorxiv.org/content/10.1... #microsky
biorxiv.org
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Syma Khalid @sykhalid.bsky.social · 02/02/2026
A gift of an article from the inimitable Colin Kleanthous as he says a moving farewell to his laboratory. A brave, unrepentant and scientifically brilliant journey is described - please have a read, friends. www.jbc.org/article/S002...
jbc.org
Bacteriocin biophysics: From protein-protein interaction models to navigators of the bacterial cell envelope
Bacteriocins are toxins deployed by bacteria to kill their competitors. Here, I reflect on my laboratory’s work on protein bacteriocins and their immunity proteins from Gram-negative bacteria. We unco...
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 02/02/2026
iCASE PhD project available in my lab, combining microscopy method development with high throughput screening of bacteria. Applications to antibiotic drug discovery and screening for new cell division proteins. Collaboration with Cairn Research #microsky #bioimaging www.findaphd.com/phds/project...
findaphd.com
Image-based bacterial phenotyping and antimicrobial drug discovery using smart high-throughput super-resolution microscopy at University of Warwick on FindAPhD.com
PhD Project - Image-based bacterial phenotyping and antimicrobial drug discovery using smart high-throughput super-resolution microscopy at University of Warwick, listed on FindAPhD.com
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Rob Fagan @robfagan.bsky.social · 01/02/2026
Finally comms from UKRI - on a Sunday evening. No mention of the hundreds of ECRs who will see their careers ended by these decisions and notably no explanation for MRC retrospectively applying cuts and wasting all the work that went into writing, reviewing and scoring applications - £millions
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Martin Loose @nartimsoole.bsky.social · 26/11/2025
Happy to share a new preprint from the lab: Marko became interested in whether the disordered linker of FtsZ might influence how the protein organizes into the Z-ring. And this led to some surprising findings! Preprint: www.biorxiv.org/content/10.1...
biorxiv.org
Charge segregation in the C-terminal linker of FtsZ enables Z-ring scaling with prokaryotic cell width
Bacterial cytokinesis is orchestrated by the Z-ring, a cytoskeletal structure formed by treadmilling filaments of the tubulin-like GTPase FtsZ. During assembly, filament curvature must match the cell ...
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allepasse.bsky.social @allepasse.bsky.social · 26/01/2026
I’m happy to share the main result of my PhD, which you can find on bioRxiv www.biorxiv.org/content/10.6.... If you are interested in learning about a new way to perform DNA-PAINT multiplexing, which we call Combi-PAINT, or if you are interested in the study of mRNA conformation, keep reading! 1/10
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 26/01/2026
SAVE THE DATE! The next instalment of the long-running Bacterial and Archaeal Cell Division Conference will take place at www.marriott.com/en-us/hotels..., Italy from the 20th to 23rd of April 2027. Organisers: me, @s-lab.bsky.social, @awehenkel.bsky.social. More info soon. #MicroSky
marriott.com
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