Endoplasmic reticulum exit sites (ERES) are dynamic platforms that coordinate not only protein trafficking but also protein quality control and signaling functions. ERES size, composition, architecture, interactions, and activity must be precisely remodeled in response to myriad pathophysiological cues to finely tune cellular homeostasis. However, the molecular mechanisms underlying this regulation remain incompletely understood. Coat protein complex II (COPII) mediates many ERES functions, and its subunits and client cargos are subject to spatiotemporal regulation by post-translational modifications (PTMs) that modulate ERES assembly, cargo selection, and inter-organelle communication. In this article, we review recent advances in understanding how PTMs control the organization and functional versatility of ERES and the COPII system.

Structures of a protein used to make crucial sugar-carrying lipids uncover a surprising reaction mechanism and a built-in brake that emerged during primate evolution.

British Journal of Cancer - Targeting Siglec-engaging immunosuppressive sialoglycans to suppress prostate cancer bone metastasis

Nature Cancer - In this Review, van Vliet and colleagues provide an overview of aberrant glycosylation as a hallmark of cancer cells, focusing on its impact for cancer immunity and therapeutic...

The mammalian brain is enveloped in a dense, charge-rich glycocalyx that orchestrates cell signalling, migration, and synaptic stability. Transcriptom…

An efficient, lesion-responsive micelle-exosome system was designed for intracartilage drug delivery.

Botulinum toxins vary in oral toxicity, but the reasons are unclear. Here, the authors show that the differences can be due to variations in one of the toxin’s components, the hemagglutinin complex, w...

Guidance for optimizing lipid nanoparticle formulations is derived using sophisticated biophysical techniques.

N-glycans on glycoRNAs prevent innate immune sensing of endogenous small RNAs, and the natural mechanism they use demonstrates how glycoRNAs exist on the cell surface and in the endosomal network with...

Abstract. The H11 antigens, located on the intestinal microvilli of Haemonchus contortus, comprise a group of homologous aminopeptidases essential for the

Nature - Super-resolution technique works with off-the-shelf optical microscopes.

Sharma, Jiao and colleagues report glycosylation of small RNAs within exosomes and propose a glycosylation-dependent mechanism for RNA targeting into exosomes, which may influence intercellular commun...

an AI researcher going back to school for immunology

The composition and potential roles of extracellular vesicles released by gut archaea are poorly understood. Here, Weinberger et al. show that extracellular vesicles produced by human gut-derived meth...

The carbohydrate 3D structure-prediction tools (builders) at GLYCAM-Web ([glycam.org][1]) are widely used for generating experimentally-consistent 3D structures of oligosaccharides suitable for data i...

Clarke et al. presents a framework for spatial analysis of the metabolome, lipidome, and glycome from a single tissue section using mass spectrometry imaging. Applying this approach, they revealed reg...

A molecule designed to activate iron locked up in organelles called lysosomes and thereby induce cell death might offer a way to tackle treatment-resistant cancer.

An RNA-binding protein on leukemia cells provides an effective target in mouse models.

The LPD-3 complex structure reveals protein–lipid interactions that suggest a model for how the native LPD-3 complex mediates bulk lipid transport and provides a foundation for mechanistic studies of ...

Disruption of mucin-domain glycoprotein expression and function in the endothelial glycocalyx are associated with ageing and Alzheimer’s disease, leading to dysregulated blood–brain barrier function.

Cell Surface Map - Distribution and organization data on immune cell surface proteins

A super-resolution method for single-molecule detection and tracking of glycans on the surface of living cells is developed. Glycan’s abundance and mobility are used to explore differences in glycosyl...

The ability to rapidly analyze complex mixtures of glycans derived from glycoproteins is important, but techniques are often laborious and require multiple glycan derivatization steps. Here, we descri...

Nature Methods - A self-driving multiresolution light-sheet microscope enables the simultaneous observation and quantification of cellular and subcellular dynamics in the context of intact and...

Glycobiology is rooted in the study of monosaccharides, Angstrom-sized molecules that are the building blocks of intricate glycosylation patterns. Glycosylated biomolecules form the glycocalyx, a dens...

Cells have evolved mechanisms to distribute ~10 billion protein molecules to subcellular compartments where diverse proteins involved in shared functions must assemble. Here, we demonstrate that prote...

Carbohydrate sulfation plays a pivotal role in modulating the strength of Siglec–glycan interactions. Recently, new aspects of Siglec binding to sulfated cell surface carbohydrates have been discovere...

Climate change-enforced drought stress conditions and diseases caused by pathogens often co-occur and represent one of the greatest challenges in plant science. Wilt pathogens that colonize water-cond...

Abstract. Central to immune recognition is the glycocalyx, a glycan-rich coat on all cells that plays a crucial role in interactions that enable pathogen d