Large language models (LLMs) are rapidly changing academic research, raising questions of who is adopting these tools and under what conditions. Th...

Extension of N- and O-glycans with linear sialic acid polymers (polySias) was described on a limited number of mammalian glycoproteins, including the α2,8-polysialyltransferases ST8Sia II and ST8Sia IV. Previous in vitro analyses have shown that ST8Sia IV from the salmonid fish Coregonus maraena (Cma) had high polysialylation activity and broader donor substrate specificity compared to its human counterpart. In this study, we investigated whether the fish ST8Sia IV was able to polysialylate N- and O-glycans of glycoproteins with a focus on its autopolysialylation capacity. Using a combination of strategies, including plant-based glycoengineering, we found that the Cma ST8Sia IV is able to use both types of acceptors for the formation of long polySia chains with a degree of polymerization of >40 consisting of N-acetylneuraminic acid and N-glycolylneuraminic acid. Given the importance of polySias in multiple health and disease states, the Cma ST8Sia IV represents a useful biocatalyst with applications in the fields of biosafe therapeutics and glycobiology.

The mammalian brain is enveloped in a dense, charge-rich glycocalyx that orchestrates cell signalling, migration, and synaptic stability. Transcriptom…

The transport and Golgi organization 2 (TANGO2) gene has been identified to cause a rare recessive genetic disorder known as TANGO2-deficiency disorder (TDD). TDD is a multi-systemic disorder that ca....

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“Exercise in absurdity” reveals flaws in Google Scholar’s productivity metrics

Clarke et al. presents a framework for spatial analysis of the metabolome, lipidome, and glycome from a single tissue section using mass spectrometry imaging. Applying this approach, they revealed reg...

Glycosylation is an important post-translational modification catalyzed by glycosyltransferases (GTs), which comprise a large group of enzymes with diversified specificity. Most GTs show high specifi...

AbstractBackground and Aims. Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract, established as a risk facto

‘When academics fear travelling or partnering with US institutions, the impacts ripple through the entire global knowledge ecosystem,’ one says

Nature Communications - While DIA has advanced standard proteomics, its application in glycoproteomics has remained limited. Here, the authors present an optimized narrow-window DIA method for...

Disruption of mucin-domain glycoprotein expression and function in the endothelial glycocalyx are associated with ageing and Alzheimer’s disease, leading to dysregulated blood–brain barrier function.

Read this JoVE article on Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Rhobo6 is a cell-impermeable small-molecule fluorophore that displays reversible fluorogenic binding to glycans, making it a general, wash-free, and non-perturbative label for the extracellular matrix...

Carbohydrate sulfation plays a pivotal role in modulating the strength of Siglec–glycan interactions. Recently, new aspects of Siglec binding to sulfated cell surface carbohydrates have been discovere...

Bennett et al. develop a statistical approach for analyzing comparative glycomics data that accounts for inherent data dependencies, yielding robust and sensitive results. This approach can identify h...

Chondroitin sulphate (CS) is a sulphated glycosaminoglycan (GAG) polysaccharide found on proteoglycans (CSPGs) in extracellular and pericellular matrices.

FUT10 and FUT11, originally annotated as α1,3-fucosyltransferases, are actually protein O-fucosyltransferases participating in a non-canonical ER quality control pathway for EMI domain-containing prot...

The mammalian glycome is structurally complex and diverse, composed of many glycan classes such as N- and O-linked glycans, glycosaminoglycans (GAGs), glycosphingolipids (GSLs), and other distinct gly...