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Ci Ji (CJ) Lim

@cijilim.bsky.social
1.3K followers 986 following 99 posts

Associate Professor @ UW-Madison Biochemistry. www.limlab.org Telomeres, Cryo-EM, Single-molecule Biophysics. Opinions are my own. 🇸🇬

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Reposted by Ci Ji (CJ) Lim
Cynthia Wolberger @cwolberger.bsky.social · 7h
Yes, she knew it was a helix and even concluded there were 10 bp/turn, with bases separated by 34 Å. For an explanation of how you can deduce all that (and more) from these patterns, see youtu.be/2tMuMRY1oDo?...
youtu.be
Photograph 51 explained
YouTube video by Cynthia Wolberger
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Jean Léon Maître @maitrejl.bsky.social · 22h
📣Our department is hiring a new group leader!📣 Want to start your team in a vibrant scientific environment in the center of Paris? I was very lucky to do that 10 years ago, reach out if you have any question. Apply by December 18th: jpi-recruitment-curie-umr3215-u934.curie.fr
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Aaron and the Hoskins Lab at UW Madison @uwmadisonrna.bsky.social · 19h
I'm super proud of this paper that just came out in G3--all of the authors (except for me and Dennis, my collaborator at the USDA-ARS at Madison) are/were undergrads in the lab! academic.oup.com/g3journal/ad...
academic.oup.com
S. cerevisiae Cwc15p Tunes the Spliceosome Active Site for Remodeling and Catalysis
Abstract. Pre-mRNA splicing is an essential step in eukaryotic gene expression during which spliceosomes remove introns from nascent RNAs while ligating th
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UW–Madison @uwmadison.bsky.social · 20h
Congratulations to Francis Halzen, UW–Madison professor of physics and principal investigator of @icecubeneutrino.bsky.social, on being named the 2026 Nobel Laureate in physics. His work helped unlock new ways of understanding the universe and the particles known as neutrinos. @nobelprize.org
news.wisc.edu
University of Wisconsin–Madison Professor Francis Halzen named 2026 Nobel laureate in physics
Halzen is the principal investigator of the IceCube Neutrino Observatory, the world’s largest telescope.
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Daniel Clery @danclery.bsky.social · 21h
Architect of polar neutrino detector wins physics Nobel | Science | AAAS 🔭🧪 @science.org www.science.org/content/arti...
science.org
Architect of polar neutrino detector wins physics Nobel
Francis Halzen honored for work on IceCube Neutrino Observatory, used to discover ghostly particles from far off universe
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UC Berkeley MCB @berkeleymcb.bsky.social · 05/10/2026
Reminder🎗️: MCB is hiring Assistant Professors across 3 divisions! 🗓️ Application Deadline: October 19, 2026
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Ci Ji (CJ) Lim @cijilim.bsky.social · 06/10/2026
Congrats, Jason!! Happy for you!
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Addgene @addgene.bsky.social · 05/10/2026
Talk about a bright idea! 💡 Congrats to Karl Deisseroth, Peter Hegemann, and Georg Nagel on the #NobelPrize in Physiology or Medicine for foundational discoveries in optogenetics. All three openly share their remarkable tools through Addgene. Explore the tools:
twp.ai
Addgene: Optogenetics Plasmid Collection
Find optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and manipulate cellular processes.
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Reposted by Ci Ji (CJ) Lim
Nobel Prize @nobelprize.org · 05/10/2026
BREAKING NEWS The 2026 Nobel Prize in Physiology or Medicine has been awarded to Karl Deisseroth, Peter Hegemann and Georg Nagel “for their discoveries concerning light-gated ion channels and optogenetics.”
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Stuart McDaniel @stuartmcdaniel.bsky.social · 03/10/2026
“For four decades, the United States has run one of the most profitable trades in the world economy… import young people that the global labor market has underpriced—graduate students whose ability outruns their résumés—give them a place to prove themselves, and keep much of the upside”
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cryoEM papers @cryoempapers.bsky.social · 03/10/2026
Conformational diversity of collided ribosomes determines stress signaling pubmed.ncbi.nlm.nih.gov/42818177/ #cryoEM
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cryoEM papers @cryoempapers.bsky.social · 02/10/2026
Cryo-tides: synthetic peptides for cryo-EM grid preparation of multi-subunit protein complexes sensitive to the air-water interface www.biorxiv.org/content/10.64898/2026.09.30.755610v1 #cryoEM
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Julieta Rivosecchi @jurivosecchi.bsky.social · 29/09/2026
In this preprint we describe new ASOs targeting telomerase RNA hTR. These ASOs are potent inhibitors of telomerase in vitro and induce telomere shortening with reduced proliferation in cancer cells. A new tool for telomere biology studies and a promising chemistry for cancer therapies. Check it out!
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Reposted by Ci Ji (CJ) Lim
Nature Structural & Molecular Biology @natsmb.nature.com · 30/09/2026
New online: A phosphoproteome atlas of human cell lines reveals the landscape of kinase activity
dlvr.it
A phosphoproteome atlas of human cell lines reveals the landscape of kinase activity
Nature Structural & Molecular Biology, Published online: 30 September 2026; doi:10.1038/s41594-026-01877-6Koenig et al. present a phosphoproteome atlas of 33 human cell lines covering >200,000 phosphosites, advancing cellular kinase activity scoring and drug sensitivity prediction, providing a resource for cell signaling insights and precision oncology.
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Adam Bentham @adamrbentham.bsky.social · 30/09/2026
🚨 Please share! 🚨 I'm recruiting a fully funded 4-year Gatsby Sainsbury PhD studentship at Durham! 🌱🤖 Designing plant immune receptors from scratch, combining AI protein design with structural biology and plant immunity. If you're interested please get in touch! benthamlab.org shorturl.at/U2PdV
benthamlab.org
Bentham Lab — Durham University
Bentham Lab, Centre for Programmable Biological Matter, Durham University. Structural biology and de novo protein design applied to host-pathogen interactions, plant immunity, and neglected tropical d...
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American Society for Cell Biology @ascbiology.bsky.social · 30/09/2026
NIH is considering changes to how grant review results are reported, including eliminating numerical overall impact scores. Comments are due Oct. 13. Learn more and make your voice heard: www.ascb.org/science-policy/nih-pro…
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UW–Madison Department of Biochemistry @uwbiochem.bsky.social · 30/09/2026
This month, the Department of Biochemistry welcomed Rae Brown to the team of instructional faculty, whose primary role is developing and teaching undergraduate biochemistry courses. Get to know more about Rae through their Q&A! biochem.wisc.edu/2026/09/23/n...
biochem.wisc.edu
New Instructional Faculty: Rae Brown
Get to know Rae Brown through their Q&A.
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Reposted by Ci Ji (CJ) Lim
Matthew Freeman @mjafreeman.bsky.social · 30/09/2026
Yet another opportunity @dunnschool.bsky.social! We are looking for an Associate Professor If you have exciting research plans, @dunnschool.bsky.social is a great place: excellent science (and lovely people, obvs) www.path.ox.ac.uk/vacancy/asso...
path.ox.ac.uk
Associate Professorship of Cancer Biology - Sir William Dunn School of Pathology in association with the Queen’s College. - Dunn School
We are seeking to appoint an Associate Professor working in the area of the cellular and/or molecular biology of cancer, to be based in Oxford University’s Sir William Dunn School of Pathology. The ro...
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NanoTag Biotechnologies @nanotag.bsky.social · 28/09/2026
✨🔬 Want to see our JF635b-conjugated #nanobodies in action? 📄 Check out the recent NanoLetters paper by Samrat Basak @samratb.bsky.social, Roman Tsukanov @romantsukanov.bsky.social, Felipe Opazo @opazo.bsky.social et al., doi.org/10.1021/acs....
doi.org
Self-Blinking Dye Restores Efficient Use of Nanobodies in Single-Molecule Localization Microscopy
Abstract. Direct stochastic optical reconstruction microscopy (dSTORM) relies on controlled fluorophore blinking to achieve nanometer-scale resolution, yet
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Reposted by Ci Ji (CJ) Lim
Aaron and the Hoskins Lab at UW Madison @uwmadisonrna.bsky.social · 25/09/2026
Can't wait for the semester's first RNA Maxigroup meeting next Tuesday! Sarah Loerch will present her work. Thanks to @rnasociety.bsky.social , @bmolchem.bsky.social , @uwbiochem.bsky.social , and the UW Madison CMB program for funding!
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Suckjoon Jun’s lab at UCSD @junlab.bsky.social · 28/09/2026
1/5 Do you use GFP to measure protein abundance? Be very careful. Our new bioRxiv preprint shows that, under translation inhibition, fluorescence can stop tracking how much reporter protein is actually there. Please share widely.
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Max Kozlov @maxkozlov.bsky.social · 25/09/2026
SCOOP: Top researchers at Columbia, Stanford, and others thought they were elected to elite scientific societies alongside Nobel laureates. The catch? The organizations are a sham. I spent the last month diving deep into a massive academic scam spanning continents, for @nature.com
nature.com
Exclusive: Sham scientific societies are misleading star researchers
A Nature investigation reveals a network of organizations that have been building their ranks through deceptive practices.
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Nature Biotechnology @natbiotech.nature.com · 25/09/2026
Photolabile oligonucleotides with topological light gradients enable spatially resolved single-cell transcriptomics and epigenomics - @ucsantabarbara.bsky.social www.nature.com/articles/s41...
nature.com
Photolabile oligonucleotides with topological light gradients enable spatially resolved single-cell transcriptomics and epigenomics - Nature Biotechnology
A photolabile oligonucleotide system enables spatially resolved single-cell measurements to show how the epigenome regulates gene expression.
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Rasmus Jensen @rasmusjensen.bsky.social · 25/09/2026
🎉 Out today in Cell: the paper I've been looking forward to sharing for a long time 🎉 We used cryo-ET to find a molecular machine nobody knew existed on the surface of a minimal bacterium, and worked out what it does. 🧵(1/7) #TeamTomo #cryoET
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cryoEM papers @cryoempapers.bsky.social · 25/09/2026
High-resolution cryoEM of nucleosomes in nuclear extracts of mammalian cells pubmed.ncbi.nlm.nih.gov/42782067/ #cryoEM
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cryoEM papers @cryoempapers.bsky.social · 25/09/2026
On-grid enrichment strategies in cryo-EM: affinity-functionalized supports for low-abundance and challenging targets pubmed.ncbi.nlm.nih.gov/42781870/ #cryoEM
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Julieta Rivosecchi @jurivosecchi.bsky.social · 24/09/2026
We explored the role of the telomeric RNA TERRA in cancer cells that transition from telomerase to ALT to maintain telomeres. We show that TERRA levels remain unchanged, but TERRA molecules relocate to telomeres and engage in R-loops. See thread ↓ link.springer.com/article/10.1...
link.springer.com
ALT induction by ASF1 depletion is associated with TERRA accumulation at telomeres and relies on R-loops - Cell Communication and Signaling
The mechanism of Alternative Lengthening of Telomeres (ALT) relies on homologous recombination processes taking place at telomeres within ALT-associated PML bodies, or APBs. ALT cells express high lev...
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Reposted by Ci Ji (CJ) Lim
Weill Institute for Cell & Molecular Biology @weillinstitute.bsky.social · 24/09/2026
Join @weillinstitute.bsky.social and Biomedical & Translational Sciences at Cornell University! We're seeking a scientist to turn fundamental cell & molecular biology insights into therapeutic discovery. Build a bold research program with opportunities to advance human and animal health. 🧬🔬
Scientist working at the bench in research lab.https://tinyurl.com/WICMB-BTS
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Reposted by Ci Ji (CJ) Lim
Vasilii Mikirtumov @vasiliimikirtumov.bsky.social · 24/09/2026
thrilled to share that my main phd project is finally out in @natcomms.nature.com we present high-resolution structures of RyR1 in native membranes across multiple functional states by both cryo-ET/StA #TeamTomo and SPA! paper: www.nature.com/articles/s41... and a thread below⬇️⬇️⬇️
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Nahel Belgherze @wxnb.bsky.social · 24/09/2026
Unbelievable footage from NOAA’s Hurricane Hunters inside the eye of Category 5 Hurricane Polo. Simply incredible.
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Martin Steinegger 🇺🇦 @martinsteinegger.bsky.social · 24/09/2026
AlphaFold Database is expanding into pandemic preparedness. Together with NVIDIA, DeepMind, EBI et al. we exhaustively predicted ~1.7 million homo- & heterodimers across 2,812 viral proteomes, resulting in 8,028 high-confidence predictions. 📄 research.nvidia.com/labs/dbr/ass... 🌐 alphafold.ebi.ac.uk
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David Solecki @so-lets-kilab70.bsky.social · 24/09/2026
Georgios Skiniotis and I are announce a unique St. Jude faculty search: a joint effort between the CoE for Structural Cell Biology an intellectual community advancing structural imaging & the Division of Neuronal Cell Biology. CryoET/vEM + neuron cell biology = 🚀 talent.stjude.org/careers/JobD...
talent.stjude.org
Faculty position, Neuronal Cell Biology and Cryo-ET/vEM
Department of Developmental NeurobiologyCenter of Excellence for Structural Cell Biology and Neuronal Cell Biology DivisionSt. Jude Children’s Research HospitalThe Center of Excellence for...
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Drug Monkey @drugmonkey.bsky.social · 24/09/2026
It's frustrating viewing to go through the new (type 1 and type 2 competing) awards by IC at grantwitness.org/nih/analyses... remember, the budget is under CR and has been the same 2004-2006. Some ICs are doing worse, some better year over year.
grantwitness.org
Agency Pulse: Grantmaking at NIH
An independent, up-to-date, grant-level record of federal science funding disruptions in the United States.
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Ci Ji (CJ) Lim @cijilim.bsky.social · 24/09/2026
Very cool!!!
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Doug Kojetin @dougkojetin.bsky.social · 23/09/2026
Apply today to be my colleague at Vanderbilt in the Department of Biochemistry! 
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Craig Kaplan @triggerloop.bsky.social · 23/09/2026
I guess I will be interested to read this preprint or paper
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Martin Pacesa @martinpacesa.bsky.social · 21/09/2026
ʙɪɴᴅᴄʀᴀꜰᴛ2 is out, and we're not waiting for the paper. The full code drops today, free for academic and industry use. We're releasing it early so you can start designing right now, and bring its full power to the current Adaptyv competition. github.com/PacesaLab/Bi...
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Ci Ji (CJ) Lim @cijilim.bsky.social · 22/09/2026
Congratulations to Yuan and his team!
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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 18/09/2026
Really impressing MINFLUX tracking of molecular motors www.nature.com/articles/s41... #bioimaging
nature.com
Characterizing dynamics of productive dynein stepping by MINFLUX - Nature Structural & Molecular Biology
Slivka et al. track dynein’s microtubule-binding domain with minimal fluorescence photon flux, revealing transient backward ‘dips’ before steps. These dips represent microtubule release upon ATP bindi...
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Sergey Ovchinnikov @sokrypton.org · 17/09/2026
Introducing highly experimental localfold.org Building on @martinsteinegger.bsky.social af2 webgpu port, @milot.bsky.social optimizations & jax ports of af3-like models by @marielle.bsky.social, Choonghwan Lee, Julia Buhmann. WARNING: runs directly on your 💻, may drain 🪫 & eat data📱 & overheat 🔥💻
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Jaap de Roode @jaapderoode.bsky.social · 15/09/2026
So excited to share that the Department of Biology @emoryuniversity.bsky.social is hiring an open-rank faculty member in the area of ecology. Happy to answer any questions. Please share! 🧪 apply.interfolio.com/191783
apply.interfolio.com
Apply - Interfolio {{$ctrl.$state.data.pageTitle}} - Apply - Interfolio
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Claus Wilke @clauswilke.com · 15/09/2026
The Department of Integrative Biology at UT Austin has two open positions for assistant professors, one in evolutionary biology and one in mechanistic processes. Please share. Apply here: apply.interfolio.com/193270
Two Assistant Professor Positions in Evolutionary Biology and Mechanistic Processes

Description
The Department of Integrative Biology at The University of Texas at Austin seeks to fill two faculty positions in the areas of Evolutionary Biology and Mechanistic Processes, broadly defined. We are looking for candidates who are advancing (1) evolutionary biology or (2) the mechanistic understanding of organismal processes. These positions will be filled at the level of Assistant Professor.


Qualifications
Candidates should be using innovative approaches in evolutionary biology and/or the mechanistic understanding of whole-organism processes. Mechanistic processes include, but are not limited to, behavior, biomechanics, cognition, physiology, and development. The phylogenetic scope of the Evolutionary Biology position may include eukaryotes, prokaryotes, viruses, and the scale may include individual molecules to entire ecosystems and beyond. Candidates for either position may employ experimental, theoretical, or computational frameworks, or a combination thereof. Special consideration will be given to applicants with demonstrated potential for integrating across our target disciplines. 

We seek applicants with a strong record of high-quality research, publications, potential for extramural funding, and a commitment to excellence in teaching and mentorship. A PhD or equivalent is required at the time of appointment, and postdoctoral research experience is strongly recommended.
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Jens Schmidt @jenscs83.bsky.social · 15/09/2026
We are recruiting a postdoctoral fellow to study DNA damage repair via the homologous recombination pathway using quantitative cell biology approaches. The position will be initially supported for two years by the MSU Research Foundation Postdoctoral Scholars program. Please share! 1/5
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Rita Strack @ritastrack.bsky.social · 14/09/2026
I picked up my passport with visa this morning so now I can officially get excited for our conference on Next-Generation Fluorescence microscopy in Beijing this week! natureconferences.streamgo.live/next-generat... It's a really stacked program because basically everyone we invited said yes!
natureconferences.streamgo.live
Next-Generation Fluorescence Microscopy
Organized by Tsinghua University, Nature Photonics, Nature, Nature Biomedical Engineering, Nature Biotechnology and Nature Communications. The conference will highlight key areas in fluorescence micro...
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Sergey Ovchinnikov @sokrypton.org · 14/09/2026
Finally a more intuitaive way to learn pLDDT/pAE? 😎 sokrypton.github.io/protein_figh... (Character idea from @hannes-stark.bsky.social & Alex Waldherr)
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Alex Ludwig @alexludwig.bsky.social · 14/09/2026
Check out our new preprint in which we used cryoCLEM to study the native architecture of the human inactive X chromosome in RPE1 cells. Neil Brockdorff’s group from Oxford posted a similar story, performed in female stem cells. We see individual nucleosomes and other exciting things!
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Nature Reviews Molecular Cell Biology @natrevmcb.nature.com · 14/09/2026
New Online! A practical guide to studying genome function using single-molecule genomics
dlvr.it
A practical guide to studying genome function using single-molecule genomics
Nature Reviews Molecular Cell Biology, Published online: 14 September 2026; doi:10.1038/s41580-026-01017-4Single-molecule genomics methods are used to study the activity of regulatory factors on individual DNA molecules genome-wide, thereby enabling quantification of molecular heterogeneity and co-occurrence of regulatory events. This Expert Recommendation article provides a practical guide for adopting single-molecule genomics and outlines best practices.
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Daniel Kierzkowski @kierzkowskilab.bsky.social · 12/09/2026
🌱We are looking for an Assistant Professor in Plant Molecular Genetics @irbv.bsky.social If you are passionate about genetics, molecular biology, or imaging, this is your chance to join a community of researchers working on plant development, cell biology, evolution, and biodiversity 🧬🔬 🧪🍁
rh-carriere-dmz-eng.synchro.umontreal.ca
Careers
Through your undergraduate and graduate-level teaching, and your research activities, you will contribute to the faculty’s pursuit of excellence. Furthermore, you will promote your discipline and actively participate in the daily activities of a renowned institution. As such, you will:
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Terence Tao @teorth.bsky.social · 11/09/2026
A group of 25 Fields Medalists, including myself, have made a joint declaration on Math and AI: mathandai.org . We welcome additional signatories. See also this article in the Economist announcing the declaration: www.economist.com/science-and-...
mathandai.org
Declaration — Math and AI
Read the declaration and add your name.
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Doc Edge @docedge.bsky.social · 12/09/2026
Please repost: My department (quantitative and computational biology at the University of Southern California) is hiring a teaching-track faculty member. The new hire will focus on teaching statistics courses to biology students. Apply by Dec 1 usccareers.usc.edu/job/los-ange...
usccareers.usc.edu
Full-Time, Teaching-Track Faculty Position in Quantitative and Computational Biology at USC
Learn more about applying for Full-Time, Teaching-Track Faculty Position in Quantitative and Computational Biology at USC
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