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NanoTag Biotechnologies @nanotag.bsky.social · 28/09/2026

Spontaneous blinking. OneStep IF. Precise #SMLM. 🙌 Our Smart Secondaries® JF635b combine a self-blinking dye with small secondary #nanobodies for reduced linkage error, no clustering & fast OneStep IF. For #dSTORM & #MINFLUX 🔬✨ 🦙 Browse our JF635b-conjugated nanobodies here: bit.ly/4ffIkdB

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NanoTag Biotechnologies @nanotag.bsky.social · 22/09/2026

💬 “The ALFA-tag has been a game changer…” Great to hear this from Diane Lidke @dianelidke.bsky.social at The University of New Mexico School of Medicine @unm.edu! Her team uses #ALFAtag and #NbALFA for single-particle tracking & #dSTORM / #DNAPAINT 🔬 📄 #ALFAsystem 700+ publications and counting! 💪

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NanoTag Biotechnologies @nanotag.bsky.social · 07/09/2026

Greetings from #Horizons2026 in our hometown Göttingen! 🦙🔬Our team is here showcasing our #nanobody -based technologies and services. Come by our booth to explore how #NanoTag might support your research! #SingleDomainAntibody #FluoTag #SmartSecondaries #ALFAsystem @horizonsmolbio.bsky.social

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NanoTag Biotechnologies @nanotag.bsky.social · 04/09/2026

🔬It’s fantastic to see our Smart Secondaries® Halo fusion contributing to such an elegant and modular solution for fluorescence lifetime #multiplexing. Congratulations to the whole team on this impressive work 👏! Check available Halo fusion #nanobodies in our shop or contact us for custom solutions.

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NanoTag Biotechnologies @nanotag.bsky.social · 02/09/2026

#NanoTag mindset: 𝗡𝗼 𝗦𝗹𝗼𝗴𝗮𝗻𝘀. 𝗝𝘂𝘀𝘁 𝗦𝗰𝗶𝗲𝗻𝗰𝗲. We started in academia, developing #nanobodies for our own research - and we still approach our reagents as scientists: develop in-house, test thoroughly, characterise and share the data 📄. Because only reliable tools move #science forward 🚀

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Reposted by NanoTag Biotechnologies

Roman Tsukanov @romantsukanov.bsky.social · 27/08/2026

New publication alert 🚨 Our Nano Letters paper on self-blinking dyes with nanobodies for robust SMLM is now Open Access! pubs.acs.org/nalefd/artic... Commercial Nb–JF635b probes are available from @nanotag.bsky.social. Joint effort of amazing collaborators, inspired by the great @opazo.bsky.social

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NanoTag Biotechnologies @nanotag.bsky.social · 27/08/2026

🔬🫧🧠 NEW Product Alert: Targeting luminal #Syt1 with flexible readouts powered by the #ALFAsystem! Meet our new sdAb anti-Syt1 Luminal Domain ALFA-tag fusion (N4843) – combine it with different #NbALFA -based reagents for fluorescence imaging or EM. 🔗 Explore more: nano-tag.com/product/sdab...

Inside synaptic vesicle lumen sdAb anti-Syt1 Luminal Domain ALFA-tag fusion (N4843, NanoTag Biotechnologies) binds to the luminal domain of Syt1. The ALFA-tag is detected by NbALFA coupled to application-specific labels, e.g. fluorophores or nanogold particles.

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NanoTag Biotechnologies @nanotag.bsky.social · 14/08/2026

🔬 Exciting to see our sdAb tools in new #microscopy approaches! In recent Nature Biotechnology @yongdengzhang.bsky.social & colleagues introduce me4Pi-SMLM, achieving near-isotropic 3D localization precision of 2–3 nm. Happy to see our anti-ALFA, anti-GFP & anti-RFP sdAbs used in this work! #SMLM

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NanoTag Biotechnologies @nanotag.bsky.social · 17/07/2026

🔬🫧💚 #FluorescenceFriday New Product – sdAb anti-Syt1 Luminal Domain #pHluorin fusion! A pH-sensitive reporter combining our sdAb anti-Syt1 Luminal Domain with superecliptic pHluorin for real-time monitoring of #SynapticVesicles exo-endocytic cycling. ♻️ #Neuroscience #Nanobody #Synaptotagmin1

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NanoTag Biotechnologies @nanotag.bsky.social · 08/07/2026

👋 ¡Hola from Barcelona! 🇪🇸☀️ Great start to #FENS2026! Visit NanoTag at Booth #43 to explore our #nanobody tools for #Immunofluorescence, #OneStepIF, #Multiplexing, #SuperResolution & #Pulldowns. Our team looks forward to meeting you!🦙🧠✨ #SingleDomainAntibodies #Neuroscience

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Reposted by NanoTag Biotechnologies

Joachim Goedhart @joachimgoedhart.bsky.social · 01/07/2026

A High-Affinity Nanobody Recognizing mNeonGreen Enables Versatile Biochemical, Cellular, and in vivo Applications. by Steffen Frey and team: www.biorxiv.org/content/10.6...

$mNeonGreen Selector: a pulldown resin for mNeonGreen-fusion proteins. (A) Sketch of mNG Selector binding mNG fusion proteins. (B, C) Immunoprecipitation from E.coli lysate in the presence (B) or absence (C) of target protein (mNG-Halo fusion, arrowhead). Proteins were eluted in boiling SDS sample buffer. mNG Selector enables highly specific one-step capture of mNG-tagged proteins. (D) Binding assay to evaluate the cross-reactivity of the mNG Selector to other recombinant fluorescent proteins. Shown is a figure of the equilibrium bound fraction of each indicated fluorescent protein prior to buffer washing. (E) Compatibility of mNG Selector to various reagents applied during binding (top panel) or during washing steps (lower panel). mNG fluorescence is lost in the presence of 2 M Gua-HCl. The symbols below each row indicate binding efficiency: ++: strong binding, + binding, -: no binding$

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Felipe Opazo @opazo.bsky.social · 02/07/2026

New paper! 🚀 A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science. We hope you find it useful! 💚

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NanoTag Biotechnologies @nanotag.bsky.social · 02/07/2026

🇪🇸Heading to #FENS2026 in Barcelona? Visit #NanoTag at Booth #43! Discover our in-house developed #nanobody reagents for #imaging, protein analysis, and #neuroscience – from FluoTags, Smart Secondaries, rHcAbs to the ALFA System and Selector Resins 🦙🧠🔬. 💬 Stop by for a chat!

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NanoTag Biotechnologies @nanotag.bsky.social · 30/06/2026

Heading to #FEBS2026? Stop by Booth #7! #NanoTag team will be there to discuss how our #nanobody technologies can support your research🦙🔬🧪 Explore the ALFA system, FluoTags, Smart Secondaries, rHcAbs & Selector Resins. See you in Maastricht! 👋 #sdAb #Immunostaining #Microscopy #ProteinAnalysis

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NanoTag Biotechnologies @nanotag.bsky.social · 22/06/2026

Congratulations @syncellbiolab.bsky.social team on this impressive work! 👏 It's exciting to see IntAct-U-ExM revealing actin architectures with such remarkable detail. Happy to see our #ALFAsystem enabling robust post-expansion labeling & high-resolution visualization of complex actin networks. 🦙🔬

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NanoTag Biotechnologies @nanotag.bsky.social · 22/06/2026

Did you know? #NanoTag provides #CRO services for custom single-domain antibody (sdAb) discovery & protein production🦙🧬🧑‍🔬 Meet our CEO at #BIO2026 in San Diego 🇺🇸 this week at the German Pavilion or get in touch via our website to discuss your project: nano-tag.com/contact/ #VHH #ProteinProduction

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Reposted by NanoTag Biotechnologies

abberior @abberior.rocks · 15/06/2026

Multiplexing with same-species primary antibodies, solved with Premix&Stain & Smart Secondary Nanobodies. Actin · GFP tag · Golgi · NUP · Mitochondria. New: STAR deepRED & STAR 550 expand the FluoTag® portfolio. @nanotag.bsky.social #SmartSecondaries #MulticolorImaging #abberior

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NanoTag Biotechnologies @nanotag.bsky.social · 15/06/2026

🔬🌈 We're expanding the spectral range of #abberior STAR-conjugated #nanobodies! 🤩 STAR 550 and deepRED have joined the range. Choose Smart Secondaries® and FluoTag®-X4 anti-GFP in your favorite colors and #multiplex with ease. 🦙🍩 In collaboration with @abberior.rocks team! #Microscopy #STED

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NanoTag Biotechnologies @nanotag.bsky.social · 11/06/2026

#NanoTag is exhibiting at #BIO2026 in San Diego!🇺🇸 Meet our CEO Dr. Hansjörg Götzke at German Pavilion, booth 3335-AF, to explore how our 🦙 sdAb discovery platforms & protein engineering services advance life science and biomedical research. #SingleDomainAntibodies #ProteinProduction #CROservices

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NanoTag Biotechnologies @nanotag.bsky.social · 08/06/2026

🔬🫧 New tools for live-cell monitoring of #SynapticVesicle dynamics based on a #nanobody targeting the Syt1 Luminal Domain are now available in the NanoTag Shop: • FluoTag®-X2 anti-Syt1 Luminal Domain • sdAb anti-Syt1 Luminal Domain Halo fusion 🔥More anti-Syt1 Luminal Domain reagents coming soon!

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NanoTag Biotechnologies @nanotag.bsky.social · 22/05/2026

#Neurizons2026 is wrapping up today! Thanks to everyone who stopped by the #NanoTag booth to chat about single-domain antibody tools. 🔬 Big thanks to the organizers and all participants for making this event such a great opportunity to connect, share ideas, and talk #neuroscience. 🧠

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NanoTag Biotechnologies @nanotag.bsky.social · 15/05/2026

NanoTag will be at #Neurizons2026! Visit our booth to explore #nanobody-based tools for super-resolution microscopy and protein analysis 🦙🧠🔬. 👋See you at Max Planck Institute for Multidisciplinary Sciences @mpi-nat.bsky.social #Neuroscience #SingleDomainAntibody

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NanoTag Biotechnologies @nanotag.bsky.social · 11/05/2026

💡Did you know? The Super-Resolution Specialist School 2026 by @esric-imaging.bsky.social is happening this week in Edinburgh 🔬 #NanoTag is happy to contribute! Our CTO Felipe Opazo @opazo.bsky.social will give a talk on #nanobody tools & #multiplexing strategies for advanced #imaging.

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NanoTag Biotechnologies @nanotag.bsky.social · 06/05/2026

Astrocytes never fail to amaze 🫠✨ This beautiful GFAP⁺ network by Svilen Georgiev (Rizzoli Lab, UMG) was stained with our fluorescent #nanobody FluoTag®-X2 anti-GFAP, revealing an intricate, highly interconnected network of filament-rich processes 🧠 #sdAb #Microscopy #Neuroscience #Astrocytes #GFAP

Primary rat brain culture showing GFAP-positive astrocytes, stained with the fluorescent nanobody FluoTag®-X2 anti-GFAP (NanoTag #N3802). Image courtesy of Svilen Georgiev.

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NanoTag Biotechnologies @nanotag.bsky.social · 28/04/2026

At a recent conference, Nahima Saliba @gustavssonlab.bsky.social, Rice University stopped by the #NanoTag booth and shared how much she enjoys using our secondary sdAbs #SmartSecondaries® for DNA-PAINT 🔬🎨 Faster, easier #multiplexing with a pre-mixing protocol – exactly what they’re designed for 💙

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NanoTag Biotechnologies @nanotag.bsky.social · 17/04/2026

📣 Neuroscientists – something NEW for you! NanoTag expands its portfolio of neurobiological #rHcAbs 🧠. Nanobodies targeting #SYT1 and #CNPase are now available as Mouse or Rabbit IgG Fc fusions – ideal for ICC and IHC 🔬. Learn more 👉 bit.ly/4cKITOd #Neuroscience #RecombinantAntibody

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NanoTag Biotechnologies @nanotag.bsky.social · 10/04/2026

📄 A recent study in #ACSNano by @heilemannlab.bsky.social shows that 🔬STED microscopy with DNA-fluorophore labels enables intensity-based molecular counting. Happy to see our #nanobodies 🦙 FluoTag®-X4 anti-GFP and sdAb anti-ALFA contribute to this work. Read the paper here: doi.org/10.1021/acsn...

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NanoTag Biotechnologies @nanotag.bsky.social · 01/04/2026

🌸 Springtime – where flowers and ideas bloom! Whether you’re chasing big questions or exploring tiny molecules, our #nanobody tools & custom #sdAb services help you make egg-cellent progress. 🐣🔬🦙 Wishing you flourishing research and plenty of hoppy moments 🐇! #HappyEaster from #NanoTag!

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NanoTag Biotechnologies @nanotag.bsky.social · 30/03/2026

Meet #NanoTag at the #FOM2026 in Stockholm! Visit booth 24 to explore our 🦙 #nanobody -based solutions tailored for quantitative super-resolution microscopy and flexible #multiplexing 🔬. Our team is looking forward to connecting with you! #SingleDomainAntibodies #SmartSecondaries #FluoTag

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NanoTag Biotechnologies @nanotag.bsky.social · 18/03/2026

Join #NanoTag team at #FOM2026 in Stockholm 🇸🇪! Visit booth #24 to see how our single-domain antibody (sdAb) technologies bring greater precision to #microscopy and more flexibility & ease to #multiplexing 🔬 See you in Stockholm! 👋 #SuperResolution #Nanoscopy #Nanobody #Immunofluorescence

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NanoTag Biotechnologies @nanotag.bsky.social · 10/03/2026

📄🦠 New publication! In collaboration with #Takeda our synthetic single-domain antibody library enabled rapid discovery of strain-specific sdAbs against SARS-CoV-2 variants, supporting vaccine identification testing. Read more in Antibodies @mdpiopenaccess.bsky.social 👉 doi.org/10.3390/anti...

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NanoTag Biotechnologies @nanotag.bsky.social · 04/03/2026

📄🔬 Congratulations to all authors on this excellent preprint showcasing #NanoFLex, a FLIM strategy that enables lifetime-based separation of multiple targets in a OneStep-IF using our #SmartSecondaries fused to different #HaloTag variants. 👏 We’re proud that SmartSecondaries® power this workflow☺️

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NanoTag Biotechnologies @nanotag.bsky.social · 24/02/2026

We’re having a fantastic time at #BPS2026 in San Francisco 🌁 Last chance to stop by booth 720 to meet the #NanoTag team and learn how our nanobody-based reagents 🔬 and sdAb services 🦙 can advance your research. Thanks for the great discussions and the positive feedback on our #sdAb technologies💙!

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NanoTag Biotechnologies @nanotag.bsky.social · 19/02/2026

Did you know #multiplex IF can be done in just one incubation step? ❌ No sequential staining ❌ No host-species limits ❌ No secondary-induced clustering 🔬Pre-mix your favourite primary antibodies (even same-species) with Smart Secondaries® #nanobody & apply directly to your sample for OneStep IF.🤩

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NanoTag Biotechnologies @nanotag.bsky.social · 13/02/2026

Neuroscientists - looking for a high-affinity gift?💝 Inside the postsynapse, proteins crowd the show PSD95’s the star - but hard to know. IgG antibodies knock, but can’t get through Too bulky to reach the target they pursue. But FluoTag®-X2 anti-PSD95 slips right in Binds PSD95. Precise within.

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NanoTag Biotechnologies @nanotag.bsky.social · 12/02/2026

Heading to #BPS2026 in San Francisco? Visit NanoTag team at booth 720 to explore our single-domain antibody #sdAb tools: ALFA® System, FluoTags®, Smart Secondaries® & Selector Resins. See you! 👋 @biophysicalsoc.bsky.social #Nanobody #Multiplexing #SuperResolution #Microscopy #AffinityPurification

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NanoTag Biotechnologies @nanotag.bsky.social · 06/02/2026

📄This new Journal of Microscopy 🔬 paper uses rigorous single-molecule assays to benchmark secondary nanobodies and shows NanoTag #SmartSecondaries® deliver high-affinity binding, efficient saturation, and exceptionally low off-target labeling for robust #multiplexed immunoassays 💪

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NanoTag Biotechnologies @nanotag.bsky.social · 03/02/2026

💬 Thank you, Bhanu Prakash Potlapalli, for the great feedback on the ALFA® system! 😊 Applied in a CRISPR–dCas9 DNA imaging approach for efficient labeling of repetitive DNA sequences and live-cell imaging of plant telomeres doi.org/10.1093/jxb/... Explore #ALFAsystem 👉 nano-tag.com/technology/t...

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NanoTag Biotechnologies @nanotag.bsky.social · 28/01/2026

Did you know? #NanoTag offers custom single-domain antibody (sdAb) discovery services, including alpaca immunization🦙and synthetic library–based approaches🧬backed by over a decade of experience in the field 💪. Discuss your project with our experts: nano-tag.com/contact/ #CRO #Nanobody #VHH #sdAb

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NanoTag Biotechnologies @nanotag.bsky.social · 21/01/2026

🔬 Join Imaging Bites seminar 28 Jan at 12:30 at @kingscollegelondon.bsky.social. Prof. Dr. Felipe Opazo @opazo.bsky.social presents #OneStep-IF, a simplified approach to highly #multiplexed IF using same-species primaries and Smart Secondaries® premixes. Register here: www.kcl.ac.uk/events/imagi...

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Reposted by NanoTag Biotechnologies

Felipe Opazo @opazo.bsky.social · 22/12/2025

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

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NanoTag Biotechnologies @nanotag.bsky.social · 22/12/2025

🎄 A glimpse into #NanoTag Christmas dinner - Glühwein outdoors, a shared dinner, and Schrottwichteln 🎁♻️ where funny, used items are exchanged and find a new home. The past year has been special for us as we celebrate 10 years. Grateful for our team, customers, and partners. Here’s to what’s next 🥂

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NanoTag Biotechnologies @nanotag.bsky.social · 11/12/2025

#CellBio2025 in Philadelphia has come to a close! Thanks to everyone who stopped by the #NanoTag booth 🦙 – great conversations and insights all around. We were excited to hear how our sdAb tools are driving your new discoveries 📄👏 #SingleDomainAntibody #ALFAsystem #SmartSecondaries

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NanoTag Biotechnologies @nanotag.bsky.social · 06/12/2025

What most people don’t see is the behind-the-scenes effort- not magic, just #teamwork. Karina & Steffen have #NanoTag booth ready for #CellBio2025 in Philadelphia. Starting Sunday, discover how our single-domain antibody solutions can support your research🔬🦙. See you ascbiology.bsky.social 👋

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NanoTag Biotechnologies @nanotag.bsky.social · 25/11/2025

Attending #CellBio2025 in Philadelphia Dec 6–10? Visit #NanoTag team at booth #1117 to explore our #sdAb technologies & ready-to-use reagents: ALFA® System, FluoTags®, Smart Secondaries®, Selector Resins. See you there! 👋 @ascbiology.bsky.social #Nanobody #CellBiology #Immunofluorescence #PullDown

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NanoTag Biotechnologies @nanotag.bsky.social · 21/11/2025

Join our CTO, Felipe Opazo @opazo.bsky.social, at #MBSJ2025 @mbsj-official.bsky.social as he presents new super-resolution #multiplexing advances powered by #SmartSecondaries®. 🔬Discover flexible, scalable and precise multi-target imaging. #Nanobody #NanoPlex #SUMPAINT #SuperResolution #Microscopy

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NanoTag Biotechnologies @nanotag.bsky.social · 14/11/2025

🌱🔬 #FluorescenceFriday Beautiful plant microscopy revealed with the ALFA-tag & NbALFA. A recent study by @plantubiquitin.bsky.social et al in Plant Commun shows how well #ALFA®system works in planta. From imaging #SuperResolution to protein interactions, degradation and more. doi.org/10.1016/j.xp...

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NanoTag Biotechnologies @nanotag.bsky.social · 30/10/2025

This #Halloween 🦙🧛‍♂️ Alpacula brewed up #SMLM treat! No more horror stories of no blinking & black holes ⚫️🔬Our single-domain anti-BOOdies 👻 #FluoTags and #SmartSecondaries now glow with self-blinking JF635b✨, delivering frightfully precise and simple #dSTORM imaging. 👉 bit.ly/4ffIkdB

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NanoTag Biotechnologies @nanotag.bsky.social · 28/10/2025

#NanoTag is exhibiting at #BIOEurope 2025 🇦🇹 Nov 3–5! Meet our team in Vienna at the German Pavilion, booth C120-18, to explore how our 🦙 sdAb discovery platforms and protein engineering services advance life science and biomedical research. #SingleDomainAntibodies #ProteinProduction #CROservices

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