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Scientists have been modeling proteins using machine learning for years. Advances in AI have made these models increasingly accurate, leading to modern tools like AlphaFold. Read an introduction to AlphaFold and how it can help you in our newest blog post!

twp.ai

blog.addgene.org

An Introduction to AlphaFold

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Addgene @addgene.bsky.social · 21h

Looking for more control over GPCR signaling cascades? Three new kits for Gαs Inhibitory Probes, created by the García-Marcos lab (@bumedicine.bsky.social), are now available! Learn more and find plasmids here:

twp.ai

Addgene: Gαs Inhibitory Probes Kits

Use to study the spatiotemporal organization of endogenous Gαs signaling or to achieve chemically-induced inhibition of Gαs.

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Addgene @addgene.bsky.social · 29/09/2026

Structure determines function, but what if you only have a sequence? In comes AlphaFold to save the day 🦸 AlphaFold is an AI-backed protein structure prediction model that only requires a protein sequence to get started. Learn some of the basics in our latest blog post!

twp.ai

blog.addgene.org

An Introduction to AlphaFold

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Addgene @addgene.bsky.social · 28/09/2026

27,000+ plasmids, curated from Addgene's repository of 170,000+ constructs — spanning CRISPR, imaging and reporters, and gene expression — now available as ready-to-pipette Transfection-Ready DNA. And more to come! twp.ai/4hvlat #Plasmids #CRISPR

Addgene graphic highlighting 27,000+ Transfection-Ready DNA plasmids available across CRISPR, imaging and reporter, and gene expression applications.

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Addgene @addgene.bsky.social · 25/09/2026

Tired of cross-reactivity muddying your UNC5 family results? New from @ipiproteins.bsky.social: Anti-UNC5A [IPI-mUNC5A.50], recognizing mouse and human UNC5A with no cross-reactivity to other UNC5 family members. Recommended for FC, ICC, and IHC. twp.ai/4huoe9

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Addgene @addgene.bsky.social · 23/09/2026

Every Transfection-Ready DNA order is backed by whole-plasmid NGS — not Sanger, not a restriction digest. That same verified DNA arrives ready to pipette, the moment it lands on your bench. twp.ai/4hvlcY #NGS #QualityControl

Addgene graphic promoting Transfection-Ready DNA with the message “Backed by whole plasmid sequencing” on a dark blue background with stylized circular DNA illustrations.

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Addgene @addgene.bsky.social · 22/09/2026

New pooled library! 🧬 Genome-scale CRISPR interference and perturb-seq of genes in primary human CD4+ T cells. Thanks to ronghuizhu.bsky.social, Emma Dann, Jonathan Pritchard, Marson Lab, and colleagues!

twp.ai

Addgene: htCRISPRi-Puro Libraries

Use this genome-scale CRISPRi library for inhibiting transcription of expressed genes in human T cells.

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Addgene @addgene.bsky.social · 21/09/2026

Heading to #iZIM2026 (Oct 2–4)? Visit e-nnovation Life Sciences’ booth to learn about Addgene’s zebrafish collection of 3,000+ sequence-verified plasmids. Addgenies Andrew Donaldson, Maria Genco, PhD, and Ashley Waldron, PhD, will be there too! twp.ai/4hurQ7 #Zebrafish

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Addgene @addgene.bsky.social · 18/09/2026

Congrats on the Blue Flame Award, @gerryhammond.bsky.social, and thank you for helping advance open science through sharing with the research community! 🔥 #OpenScienceWeek

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Addgene @addgene.bsky.social · 18/09/2026

We now offer Transfection-Ready DNA — NGS sequence-verified plasmid DNA from Addgene, ready to go for your next transfection. Available now: twp.ai/4hvlbn #Plasmids #Transfection

Addgene graphic promoting 27,000+ plasmids available as Transfection-Ready DNA, featuring a pipette and “Browse Plasmids” call to action.

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Addgene @addgene.bsky.social · 16/09/2026

Just published! 💡 Rhobin: a series of high-affinity, reversible rhodamine-binding proteins for fluorescence microscopy. New from Bo Huang, Jon Grimm, Luke Lavis, Dyche Mullins et al., and colleagues. twp.ai/4hvigs

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Addgene @addgene.bsky.social · 15/09/2026

Why grow it when you can just pipette it? Get the same plasmids you already order from Addgene — ready the moment they arrive. Learn more: twp.ai/4hvlYK #Plasmids #Transfection

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Addgene @addgene.bsky.social · 15/09/2026

Excited to announce the launch of Transfection-Ready DNA — giving academic and industry researchers access to 27,000+ plasmids from Addgene's trusted repository as high-purity, NGS sequence-verified DNA. Read the press release: twp.ai/4hvlXn #Plasmids #Transfection

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Addgene @addgene.bsky.social · 14/09/2026

What happens when scientists share their materials? Faster, more reproducible research. That’s the spirit of #OpenScienceWeek, and it’s been Addgene’s mission for 20+ years. Learn more about what drives our work: twp.ai/4hvnqx.

twp.ai

Addgene: Mission

Learn about Addgene's mission, services, and history. Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

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Addgene @addgene.bsky.social · 11/09/2026

Lantern🏮a new tool for RNA and protein proximity labeling from Peng Zou and colleagues! Read about its applications for mapping local transcriptomes and proteomes, and find plasmids here: www.addgene.org/browse/artic...

addgene.org

Addgene: Directed evolution of Lantern enables rapid RNA and protein proximity labeling.

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Addgene @addgene.bsky.social · 10/09/2026

Our neuroscience tools are on the ground at the Chinese Neuroscience Society Annual Meeting 2026 (Sept. 10–13)! Beijing Zhongyuan Ltd., Addgene’s authorized distributor in China, will share our materials and provide guidance on deposits, MTA processing, and China import and customs support. #CNS2026

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Addgene @addgene.bsky.social · 10/09/2026

Congratulations to Michael J. Fox on the 2026 Lasker~Bloomberg Public Service Award for his Parkinson’s research advocacy! 🎉 @laskerfdn.bsky.social The Michael J. Fox Foundation shares hundreds of plasmids relevant to Parkinson’s research:

twp.ai

Addgene: Michael J Fox Foundation (MJFF) Plasmid Collection

Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation

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Addgene @addgene.bsky.social · 09/09/2026

We love to see it! 👀 Neuro-seeER is a fluorescent reporter for tracking neuronal estrogen response dynamics in vivo. Thanks @endoeartha.bsky.social, @acara333.bsky.social, @edvanveenphd.bsky.social et al. for sharing! Learn more 👇 and find the plasmids here 🧬: www.addgene.org/browse/artic...

addgene.org

Addgene: A bifunctional optical reporter for tracking estrogen response dynamics in neurons.

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Addgene @addgene.bsky.social · 09/09/2026

Looking for a skimmable version of our webinar “CRISPR Pooled Libraries: Optimizing Knockout Library Design,” with Laura Drepanos from Broad Institute? The summary is here, along with a full transcript of the live Q&A:

twp.ai

blog.addgene.org

Julianna and Jacquere: New and Improved Genome-Wide CRISPR Knockout Libraries

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Addgene @addgene.bsky.social · 08/09/2026

DNA prep shouldn't be the hardest part of your experiment. Something's changing in our catalog on September 15. We can't wait to show you what's next. #Plasmids #Transfection

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Addgene @addgene.bsky.social · 08/09/2026

Like their predecessors, CRISPR knockout libraries Jacquere and Julianna are named after a wine 🍷 and cheese 🧀. But they’re also smaller, more effective, and still cover the entire human or mouse genome. Learn about these improvements and how to use these libraries here:

twp.ai

blog.addgene.org

Julianna and Jacquere: New and Improved Genome-Wide CRISPR Knockout Libraries

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Addgene @addgene.bsky.social · 02/09/2026

What do worm transgenesis, axon guidance, bacterial immunity, and astrocytes have in common? They're all in our new Hot Plasmids post! We've got hot tools from @ipiproteins.bsky.social, Aaron Whiteley, Melissa Cooper, PhD, Shane Liddelow, and more:

twp.ai

blog.addgene.org

Hot Plasmids: September 2026

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Addgene @addgene.bsky.social · 02/09/2026

Nyla Naim’s onboarding started with a big one: a deposit of 3,500 transcription factor plasmids from Julia Joung and Feng Zhang, Addgene’s largest collection ever. That story kicks off our new video series, Built to Share. 🎬 Watch the first video: twp.ai/4huEtc

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Addgene @addgene.bsky.social · 01/09/2026

Are you working with C. elegans? 🪱 Check out our updated Worm Collection page, now including tables for biosensors and reporters, codon-optimized, genome engineering, and neuroscience plasmids! Browse the Worm Collection here:

twp.ai

Addgene: Worm Plasmids and Resources

Browse Addgene’s collection of plasmid tools, kits, and resources for use in C. elegans research. Including genome engineering, codon-optimized, biosensors and reporters, and neuroscience plasmids.

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Addgene @addgene.bsky.social · 01/09/2026

Nick! Shred! Cut! Read about the latest prime editors from David Liu Broad Institute, RNA-targeted cell killing with Cas12a2 from Yang Liu @utah.edu & Doudna Lab, CoCUT&Tag chromatin mapping from Derek Janssens @vai.org, and more in our latest Hot Plasmids:

twp.ai

blog.addgene.org

Hot Plasmids: September 2026

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Addgene @addgene.bsky.social · 26/08/2026

20,000+ CRISPR plasmids. 1,000+ depositor labs. 43 countries. 300,000+ plasmids shipped. At CRISPR Frontiers 2026, we shared 12 years of tool-sharing data, then explored next-gen editors, CASTs, and an enzyme that turns RNA sensing into cell killing. Full recap:

twp.ai

blog.addgene.org

What's New in Genome Editing: Takeaways from CRISPR Frontiers 2026

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Addgene @addgene.bsky.social · 25/08/2026

Fourteen years after Cas9, is CRISPR still just about cutting DNA? Not even close. Our recap of CRISPR Frontiers 2026 at @cshlnews.bsky.social covers break-free DNA insertion, RNA-sensing cell killing, and the delivery problem the field is working to solve:

twp.ai

blog.addgene.org

What's New in Genome Editing: Takeaways from CRISPR Frontiers 2026

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Addgene @addgene.bsky.social · 20/08/2026

Mapping how axons find their way and synapses get organized?🧠 @ipiproteins.bsky.social just added 6 new antibodies to the mix: Netrin-1, Netrin-G1, Slit-1 (x2 clones), and Semaphorins 5A/5B. Take a look:

twp.ai

Addgene: IPI Antibody Collection

Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene and IPI.

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Addgene @addgene.bsky.social · 18/08/2026

Hit a login screen while grabbing an Addgene sequence lately? It’s fair to ask why. Automated tools were scraping sequence data in bulk, far outside any lab. A free account keeps this resource secure for the researchers who rely on it. Why it matters:

twp.ai

blog.addgene.org

Protecting the Science You Share: Why Sequence Data Requires a Login

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Addgene @addgene.bsky.social · 14/08/2026

Can’t tell PE3b from PE8c? Our updated post on prime editing, the CRISPR-based search-and-replace genome editing technique, includes a family tree to help you understand the differences between these editors. Read it here:

twp.ai

blog.addgene.org

Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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Reposted by Addgene

Bas Bargmann @bas-bargmann.bsky.social · 11/08/2026

Get yer PR1::RUBY here! www.micropublication.org/journals/bio... Available through Addgene and ABRC (ABRC is still bulking up the seed) www.addgene.org/255430/ abrc.osu.edu/stocks/1211866

micropublication.org

A Non-Invasive Pathogen‑Responsive RUBY Reporter Enables Visible Monitoring of PR1 Activation | microPublication

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Addgene @addgene.bsky.social · 13/08/2026

Missed last week's blog post? From the first deposit in 2012 to our most recent in 2026, we've watched the CRISPR field grow and mature. Read the blog post for more data and insights:

twp.ai

blog.addgene.org

CRISPR Deposits, Distributions, and Other Stats: A Snapshot of the State of CRISPR

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Addgene @addgene.bsky.social · 13/08/2026

At #CRISPR2026, Allen Tao from the David Liu Lab of Broad Institute presented on enhanced prime editing through AI-guided redesign of laboratory-evolved reverse transcriptases.

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Reposted by Addgene

Alliance of Genome Resources @alliancegenome.bsky.social · 12/08/2026

Looking for model organism resources to follow on Bluesky? Here's a starter pack for you! go.bsky.app/Npxsd7h

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Addgene @addgene.bsky.social · 12/08/2026

Programmable insertion of large DNA segments is still one of genome editing’s toughest challenges. This morning at #CRISPR2026, Martin Jinek opened the CRISPR Technologies session, tracing the field’s path from Cas9’s 2012 debut through base and prime editing.

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Addgene @addgene.bsky.social · 12/08/2026

We've made it to @cshlnews.bsky.social! Excited to spend the week talking CRISPR and genome engineering with researchers from around the world. Come find our table, say hi to Andrew Hempstead and @elenamimo.bsky.social, and check out our poster (#101) on research tool sharing today. #cshlcrispr26

Andrew Hempstead and Elena Miñones Moyano smiling at the Addgene table at the CSHL CRISPR conference.

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Reposted by Addgene

Michael Marty @michaelmarty.bsky.social · 12/08/2026

New paper from the group on design of fluorescent nanodiscs with diverse properties: doi.org/10.1021/acsc.... Congrats to Estevan and the team! All plasmids are on @addgene.bsky.social www.addgene.org/Michael_Marty/ There's also a very useful plasmid for GFP-Annexin hidden in here

doi.org

Design of Fluorescent Membrane Scaffold Proteins for Nanodiscs

AbstractNanodiscs are nanoscale lipid bilayer membrane mimetics surrounded by two membrane scaffold proteins (MSPs). They are widely used as soluble casset

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Addgene @addgene.bsky.social · 11/08/2026

It’s prime time! 😎 New AI-redesigned PE8, from the David Liu lab at Broad Institute, inspired us to update our prime editing post — and boy has this field been busy! Check it out:

twp.ai

blog.addgene.org

Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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Addgene @addgene.bsky.social · 10/08/2026

Brighten up your day ✨ New tools from Cagla Eroglu’s lab @caglaeroglu.bsky.social for imaging astrocytes and microglia, centered on Hevin/SPARCL1: twp.ai/4huJKq

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Addgene @addgene.bsky.social · 10/08/2026

Power up your protein purification with a new SUMO protease construct from @cdbahl.com and team! Find individual plasmids here: www.addgene.org/browse/artic... Or get the full benchmark set: www.addgene.org/kits/bahl-su...

addgene.org

Addgene: Facile, high-throughput protein purification enabled by high-efficiency protease elution.

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Addgene @addgene.bsky.social · 07/08/2026

We’re heading to the 2026 BRAIN Initiative Conference in Rockville, MD next week! Come find Marcella Patrick and Meghan Rego. We’ll be talking plasmids, viral vectors, recombinant antibodies, and sharing tools for neuroscience research. 🧠 #BRAINConference #studyBRAIN #Neuroscience

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Addgene @addgene.bsky.social · 06/08/2026

Our CRISPR collection has grown to over 20,000 plasmids from 1,400 labs worldwide 🌎. Together, they offer a fascinating snapshot of the state of the CRISPR field. See more in our latest blog post:

twp.ai

blog.addgene.org

CRISPR Deposits, Distributions, and Other Stats: A Snapshot of the State of CRISPR

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Addgene @addgene.bsky.social · 06/08/2026

Heading to the Genome Engineering: CRISPR Frontiers meeting at @cshlnews.bsky.social next week? Stop by our table to meet @elenamimo.bsky.social and Andrew Hempstead, and check out poster #101 on Wednesday about how researchers share and adopt CRISPR tools through Addgene. #cshlcrispr26 #CRISPR

Addgene graphic promoting the Genome Engineering: CRISPR Frontiers meeting, August 11–15, 2026, in Cold Spring Harbor, NY, with a DNA helix design on a dark blue background.

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Addgene @addgene.bsky.social · 06/08/2026

New from Francois St-Pierre’s lab @bcmhouston.bsky.social! JEDI3sub and JEDI3hyp are voltage indicators that capture subthreshold dynamics under two-photon imaging, so you can image deeper and see more: twp.ai/4huJKn

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Addgene @addgene.bsky.social · 04/08/2026

Twenty years ago, Addgene co-founder Melina Fan, PhD, couldn’t get the plasmids she needed for her research, so she helped build a better way to share them. 🧬 On Aug. 1, Melina stepped into a new role as Chief Scientific Advisor. A thank-you and a look at what’s next:

twp.ai

blog.addgene.org

Thank You, Melina: A New Chapter After 20 Years

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Addgene @addgene.bsky.social · 31/07/2026

Great pick for #CellLineOfTheWeek — H2B fluorescently-tagged iPS cells and plasmids from @alleninstitute.org. Proud to help get these into more labs! 🧬

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Addgene @addgene.bsky.social · 31/07/2026

Great to have @allostericsite.bsky.social out at #USCCN this year, meeting the folks keeping small-scale collections going strong.🧫

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Addgene @addgene.bsky.social · 28/07/2026

New from @ipiproteins.bsky.social: a recombinant anti-ROBO2 antibody (IPI-ROBO2.47) targeting a key receptor in Slit-mediated axon guidance. Now available at Addgene, validated for FC and ICC in mouse and human: twp.ai/4ht1Pw

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