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Ci Ji (CJ) Lim

@cijilim.bsky.social

1.3K followers 987 following 99 posts

Associate Professor @ UW-Madison Biochemistry. www.limlab.org Telomeres, Cryo-EM, Single-molecule Biophysics. Opinions are my own. 🇸🇬

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Ci Ji (CJ) Lim @cijilim.bsky.social · 31/10/2025

Halloween pumpkin carving experiment by the Lim lab. 🎃

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Ci Ji (CJ) Lim @cijilim.bsky.social · 09/02/2024

3/3: Similarly for human PRC2 complex, which is notoriously challenging to determine its cryo-EM structures - requires crosslinking, surface adsorption, etc -, by adding LEA proteins before plunge freezing, we determined cryo-EM maps of PRC2 at comparable resolutions to those previously reported.

Human PRC2 cryo-EM map determined using AavLEA1

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Ci Ji (CJ) Lim @cijilim.bsky.social · 09/02/2024

2/3: The human DNA polymerase alpha-primase is a challenging complex to determine its structure. By simply adding nematode AavLEA1 protein to our complexes before plunge freezing, we achieved the highest-resolution cryo-EM map reported for the apo complex at 3 Å.

Human DNA polymerase alpha-primase cryo-EM solved using AavLEA1

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Ci Ji (CJ) Lim @cijilim.bsky.social · 09/02/2024

1/3: Led by @kaitlynabe.bsky.social, we found small LEA proteins from nematodes and tardigrades (water bears!) to be incredibly effective in protecting two test fragile complexes - human DNA polymerase alpha-primase and polycomb repressive complex 2 - from AWI damage during plunge freezing.

Mascot of water bear to the rescue! (drawn by Kaitlyn Abe, lead author)

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