Across animal phyla, odor information is represented in the first olfactory processing center by combinatorial activation of discrete glomeruli. To examine how this organization of olfactory processin...

Two-photon calcium imaging is a standard tool for measuring neuronal population activity in vivo , yet how axial resolution, sensor expression strategy, and analysis pipeline jointly affect data accur...

The authors present a dense connectomic reconstruction of a 0.1 mm3 hippocampal CA3 volume and reveal spatial gradients, high convergence and cell-type-selective feedforward inhibition of inputs to py...

In this Analysis, Rupprecht et al. investigate the behavior of GCaMP8 variants in spike inference using several different algorithms. They highlight the role of the indicators’ linearity in the infere...

Nature Methods - In this Analysis, Rupprecht et al. investigate the behavior of GCaMP8 variants in spike inference using several different algorithms. They highlight the role of the...

A space for neuroscientists to ask and answer questions about methods in Neuroscience (this replaces our previous Slack workspace). | Prospective users: you should be able to join with an academic ema...

I wanted to like QED's new 1% ranking. I don't.

Kurzgesagt is a Youtube channel that breaks down science topics for a broad audience. They have great videos, both in terms of narrative, animation, and scientific accuracy. A few days ago, the cha…

Astrocytes modulate complex brain functions. These ubiquitous glial cells constitute a multilayered system of functional units that operate across multiple spatial scales, thereby increasing the degre...

Dendrites are the tree-like arborizations of neurons through which they receive input from other neurons. This compartmentalized anatomy has given rise to the idea that individual dendrites process…

PinkyCaMP is a red genetically encoded calcium indicator with improved brightness and photostability. Derived from mScarlet, it does not exhibit photoswitching upon blue light illumination and is ther...

I’m glad to share a PyTorch-based implementation of spike inference from calcium imaging data: CascadeTorch, now available on GitHub. The original Cascade repository remains fully supported. …

How does the brain work, and how can we understand it? To approach this big question from a broad perspective, I want to report on some ideas about the brain that marked me most over the …

Over the last 20 years, many microscopes that are capable of resonant scanning two-photon microscopy have converged on using ScanImage, a powerful software package with many strengths but also two …

Astrocytes are brain cells that are often completely overlooked and dismissed or, in the opposite extreme, presented as mysterious devices that somehow solve all problems of computational neuroscie…

Understanding behavior requires datasets that capture humans while carrying out complex tasks. The kitchen is an excellent environment for assessing human motor and cognitive function, as many complex...

One of the most amazing methods in modern neuroscience is dense volumetric electron microscopy of the brain. I have mentioned some openly accessible datasets before (see my previous blog post), but…

Researchers utilize calcium imaging to track neuronal activity in the brain, highlighting the role of calcium as a signaling molecule. Recent studies investigate intracellular calcium dynamics, par…

Three-photon in vivo imaging provides access to medial prefrontal cortex, hippocampus and spinal cord in mice at up to 1.7 mm depth of neurons, dendrites, spines, astrocytes and microglia.