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Pilhofer Lab 🦠❄️🔬

@pilhoferlab.bsky.social
1.9K followers 758 following 62 posts

Microbial cell-cell interactions | contractile injection systems | cytoskeleton | evolution | multiscale imaging | ETH Zürich - run by lab members -

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Reposted by Pilhofer Lab 🦠❄️🔬
Debnath Ghosal @debnathghosal.bsky.social · 07/10/2026
When imaging at high mag using cryo-ET, field of view reduces. How to overcome this limit? How do we get a "bird’s-eye view" without compromising resolution? Our very own @HamishBrown has a solution to this: “montage tomography with a rectangular beam!” www.biorxiv.org/content/10.6...
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Sjors Scheres @sjorsscheres.bsky.social · 06/10/2026
We have 2 new preprints on @biorxivpreprint.bsky.social that describe reconstitution of #Alzheimers paired helical filaments in experimental model systems: one in human brain organoids and one in murine primary cultures. www.biorxiv.org/content/10.6... www.biorxiv.org/content/10.6...
biorxiv.org
Templated seeding induces the formation of Alzheimer paired helical tau filaments in human brain organoids
The prion concept postulates that seeded aggregation results in the recruitment of soluble protein monomers and their assembly into amyloid filaments with the same structures as those of the seeds. He...
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Reposted by Pilhofer Lab 🦠❄️🔬
Michael Wagner @michiwagner4.bsky.social · 03/10/2026
An estimated 2·3 million new cancer cases were attributable to infections globally in 2024, which is equivalent to 12% of all cancer cases. #Vaccination www.thelancet.com/journals/lan...
thelancet.com
Global burden of cancer attributable to infections in 2024: a worldwide incidence analysis
Our findings highlight the importance of infection control to achieve cancer prevention. Scientifically proven, but often underused, approaches include HPV and HBV vaccination; testing and treatment o...
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Brett Baker @archaeal.bsky.social · 02/10/2026
Two Assistant Professor Positions in Evolutionary Biology and Mechanistic Processes - including microbes! faculty.utexas.edu/career/191568
faculty.utexas.edu
Faculty Careers - The University of Texas at Austin
Unleash your intellectual power by joining a world-renowned faculty that is solving the world's most urgent problems. At UT Austin, we provide you the freedom to blaze trails, follow your curiosity, e...
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Casper Berger @casperberger.bsky.social · 23/09/2026
We developed Adaptive Polishing for the consistent preparation of ~150 nm thin FIB-lamellae. This was done by using imaging feedback to adjust parameters for each lamella, or Adaptive Milling, rather than applying a fixed recipe. Preprint: doi.org/10.64898/202...
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Gregor Weiss @weiss-lab.bsky.social · 02/10/2026
Tiny bacteria, fascinating biology 🦠 Great work by Marcel & Fabienne from Thomas Rudel’s lab @uni-wuerzburg.de using ExM to explore Chlamydia infection. @tillepprecht.ch from our group added cryo-ET insights—the tiniest bacteria we’ve ever milled!🔍 Congrats to the team www.nature.com/articles/s41...
nature.com
Sphingolipids associate with the chlamydial nucleoid and mark developmental transitions in Chlamydia trachomatis - Nature Communications
During the developmental cycle of the pathogenic bacterium Chlamydia trachomatis, non-replicative bacterial cells (elementary bodies, EBs) alternate with replicative forms within membrane-bound inclus...
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Ben Engel @cellarchlab.com · 02/10/2026
Nice @jcellsci.bsky.social "at a glance" review about the convergently evolved organizing principles of pyrenoids, chloroplast compartments that enable diverse algae to supercharge #CO2 fixation. BONUS: It comes with a poster for your wall 🖼️. Great work @phaips.vd.st & @manondemulder.bsky.social 🌊🌾🧪
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Svetlana Dodonova @dodonova-sveta.bsky.social · 30/09/2026
New preprint from Dodonova lab 🔔! Capturing multiple states by cryo-EM❄️, we show for the first time how archaeal RNA Polymerase engages a nucleosome and forms direct contacts with histones! 🧬 Great work by Gabriele & a fantastic collaboration with Dina Grohmann! 🧵 www.biorxiv.org/content/10.6...
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Philipp Radler @radler92.bsky.social · 01/10/2026
The first paper of my PostDoc in Christa Schlepers group (@archaea-vienna.bsky.social) on the dynamics of Asgard archaea is now out in Nature! www.nature.com/articles/s41... This was a great team effort and I am looking forward to uncover the complex behavior of these cells further!
nature.com
Dynamic protrusions mediate crawling motility in Asgard archaea - Nature
Anoxic live-cell imaging shows actin-dependent crawling motility and dynamic protrusions in Asgard archaea, providing insights into cellular innovations that may have contributed to eukaryotic evoluti...
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Archaea Biology Vienna @archaea-vienna.bsky.social · 01/10/2026
Our paper on the dynamics of Asgard archaea (Promethearchaeota) is now out in Nature! Huge thanks to the reviewers for helping make the manuscript stronger. www.nature.com/articles/s41... Two “Lokis” stained for Lokiactin (FastAct, magenta), reaching toward each other to celebrate 🎉 #archaea
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Tanmay Bharat @tbharat-lab.bsky.social · 30/09/2026
Diverse S-layer anchoring mechanisms create an epiplasmic space in prokaryotic cell envelopes. Our review with @bupbuse.bsky.social and @vikramalva.bsky.social discussing a cellular compartment created by the S-layer out in Current Opinion in Microbiology. www.sciencedirect.com/science/arti...
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Asaf Levy @asaflevylab.bsky.social · 30/09/2026
In October we are starting year 8 (I think) of the virtual microbiology journal club (on Zoom) with fantatstic speakers, as usual. Most talks are about bacteria. If you (PIs, trainees, anyone interested in science) want to be included in the mailing list - shoot me an email. alevy@mail.huji.ac.il
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Florian Mayer @florianmayer.bsky.social · 11/09/2026
Excited to share our preprint on Ignicoccus cell divison: only the inner membrane divides, progeny cells accumulate in cell clusters and pop-out! www.biorxiv.org/content/10.6... Great work with the labs: @buzzbaum.bsky.social @curiousdina.bsky.social @anja1.bsky.social @tbharat-lab.bsky.social
biorxiv.org
The life cycle of an archaeon with multiple membranes
Many prokaryotes are diderms. They divide using an FtsZ division ring to simultaneously constrict physically coupled inner and outer membranes to form daughter cells with two membranes. Currently, onl...
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Mark Peifer (He, him) @peiferlabunc.bsky.social · 29/09/2026
For the last 34 years I have been on the faculty of the UNC-Chapel Hill Department of Biology and have never regretted that decision. This year we are hiring TWO new Assistant Professors in the broad area of Molecular, Cell, Developmental Biology 1/n bio.unc.edu
Front page of the website
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Kranzusch Lab @kranzuschlab.bsky.social · 10/09/2026
Stunning cryo-ET analysis from the Harrison Lab capturing every stage of how non-membrane viruses infect animal cells www.science.org/doi/10.1126/...
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George O'Toole (he/him/his) @geiselbiofilm.bsky.social · 26/09/2026
Pls share: Two tenure-track positions in Molecular Microbiology at Virginia Tech. I had a great visit there a couple of years ago! jobs.apply.vt.edu/jobs/assista...
jobs.apply.vt.edu
Assistant/Associate Professor - Blacksburg, Virginia, United States
Job Description The Department of Biological Sciences in the College of Science at Virginia Tech seeks applicants for two (2) tenure-track research and teaching positions at the Assistant/Associate ...
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Rasmus Jensen @rasmusjensen.bsky.social · 25/09/2026
🎉 Out today in Cell: the paper I've been looking forward to sharing for a long time 🎉 We used cryo-ET to find a molecular machine nobody knew existed on the surface of a minimal bacterium, and worked out what it does. 🧵(1/7) #TeamTomo #cryoET
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Ian Yen @ianyyen.bsky.social · 25/09/2026
I had a great time at this conference last year and now have the pleasure of helping organize the GRS. I would encourage colleagues to apply and spread the word!
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Omaya Dudin 𓂆 ¦🍉¦🦠¦🔬¦ @dudinlab.bsky.social · 22/09/2026
Busy week for #ProtistsOnSky After some planning with Tom Battin & @nikobiota.bsky.social The #Alpine #Microbial Workshop is finally here in #Sion! We're grateful to all funders @sciencesunige.bsky.social #EPFL @embl.org & many supportive foundations & all our collaborators who made this possible.
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Craig Kaplan @triggerloop.bsky.social · 25/09/2026
Cryo-EM peeps are living the dream www.science.org/doi/10.1126/...
science.org
In-cell structures visualize human pre-ribosome assembly in the nucleolus
In eukaryotes, ribosome biogenesis initiates within the nucleolus, a hallmark multilayered compartment of the nucleus. Structures of pre-ribosomes have been characterized ex situ, but their assembly p...
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Simonetta Gribaldo @sgribaldo.bsky.social · 23/09/2026
Interested in #archaea, #methane, #cell-envelopes? New paper out @nature.com ! We discovered an enzyme that specifically cleaves the cell wall of methanogens, revealing a new chemical structure of archaeal peptidoglycan, 50 yrs after its first description www.nature.com/articles/s41... #MicroSky 🧵👇
nature.com
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Nika Pende @nikapende.bsky.social · 23/09/2026
So happy to see this paper finally out 🥳 it took only seven years after the first purification of archaeal peptidoglycan 🤣 Congrats to all the people involved 🥂🍾 And here is the story behind the paper: communities.springernature.com/posts/cracki...
communities.springernature.com
Cracking the Archaeal Cell Wall: How a hydrolase overturned a 50-year-old paradigm
Using bioinformatics, biochemistry, NMR, as well as the newly characterized enzyme ArmA from methanogenic archaea, we revised the existing knowledge on archaeal peptidoglycan structure. At the same ti...
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Cathy Spangler @cathyspangler.bsky.social · 22/09/2026
I'm excited to share our new preprint, where we show that fine-tuning gold nanoparticle conjugation to different Fab structural elements can produce powerful, rigidly coupled, labeling tools for in-situ cryo-ET. Take a look if you want to see an AuNP in an EM map!🔬🧠 www.biorxiv.org/content/10.6...
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Maksim Kalutskii @maksimkalutskii.bsky.social · 23/09/2026
Excited to share our new preprint! 📜 Here, we used CALVADOS and ultra-CG models to show how the kinetochore establishes a robust attachment to the dynamic microtubule end. Spoiler: IDRs 🧵 1/n www.biorxiv.org/content/10.6...
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Ryo Yokoyama @yokoyama-ryo.bsky.social · 23/09/2026
Spatial proteomics reveals carboxysome adaptation and mitochondrial proteome in Paulinella chromatophora: Current Biology www.cell.com/current-biol... @currentbiology.bsky.social
cell.com
Spatial proteomics reveals carboxysome adaptation and mitochondrial proteome in Paulinella chromatophora
Using LOPIT-DC-based spatial proteomics, Cadena et al. map the sub-organellar protein landscape of Paulinella chromatophora, a cercozoan with evolutionary-early-stage photosynthetic organelles. They f...
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Buzz Baum @buzzbaum.bsky.social · 22/09/2026
We are looking for a research assistant! Come join the team: www.jobs.ac.uk/job/DSZ826/r...
jobs.ac.uk
Research Support Officer | Cell Biology | Dr Buzz Baum | LMB 2904 at MRC Laboratory of Molecular Biology
Start your UK & international job search for academic jobs, research jobs, science jobs and managerial jobs in leading universities and top...
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Earth and Planetary Sciences at ETH Zurich @eth-eaps.bsky.social · 22/09/2026
The Department of Earth and Planetary Sciences at ETH Zurich invites applications for the position of Assistant Professor (Tenure Track) of Earth and Planetary Evolution. ethz.ch/en/the-eth-z... Please apply online. The closing date for applications is 15 December 2026.
ethz.ch
Assistant Professor (Tenure Track) of Earth and Planetary Evolution
The Department of Earth and Planetary Sciences (www.eaps.ethz.ch) at ETH Zurich invites applications for the above-mentioned position.
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Swiss National Science Foundation (SNSF) @snsf.ch · 22/09/2026
🏆 Biochemist Martin Jinek is the winner of the Swiss Science Prize #MarcelBenoist 2026, considered the Swiss #Nobel Prize. He contributed to the development of molecular scissors, namely CRISPR-Cas9 technology. Read more ➡️ sohub.io/3hs4
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Martin Pacesa @martinpacesa.bsky.social · 21/09/2026
ʙɪɴᴅᴄʀᴀꜰᴛ2 is out, and we're not waiting for the paper. The full code drops today, free for academic and industry use. We're releasing it early so you can start designing right now, and bring its full power to the current Adaptyv competition. github.com/PacesaLab/Bi...
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Hiro Imachi @hiro-imachi.bsky.social · 17/09/2026
An interview article has been published in Nature. It focuses on the cultivation of Asgard archaea (formally, the phylum Promethearchaeota). www.nature.com/articles/d41...
nature.com
These bizarre, much-coveted microbes are revealing the origins of complex life
Researchers are racing to culture a menagerie of exotic organisms and finding lots of surprises along the way.
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Vienna BioCenter Scientific Training @vbcscitraining.bsky.social · 18/09/2026
Autumn PhD call is OPEN! 30 fully funded positions across diverse life science disciplines. Explore projects in Computation biology 👉 www.vbcphd.at Apply by 10 October Institute of Molecular Biotechnology, Max Perutz Labs Vienna, IMP, Gregor Mendel Institute of Molecular Plant Biology
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Pedro Beltrao @pedrobeltrao.bsky.social · 18/09/2026
Anthropic has used Claude to optimise structure prediction and design code to improve speed and memory efficiency. 2-3X speed improvements on AF3, 5X on OF3, 5X on BindCraft low memory modes to model large assemblies. All released as open source www.anthropic.com/research/cla...
anthropic.com
How Claude is uplifting biomolecular modeling
Anthropic is an AI safety and research company that's working to build reliable, interpretable, and steerable AI systems.
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Roland Hatzenpichler @environmicrobio.bsky.social · 16/09/2026
Join us at the "2027 Applied and Environmental GRC: Mechanistic Microbiology for a Complex World" July 18-23, 2027 and GRS "Mechanistic Microbiology Beyond the Model Organism" July 17-18. Applications are open! @thuls.bsky.social and I put together an awesome schedule! www.grc.org/applied-and-...
grc.org
2027 Applied and Environmental Microbiology Conference GRC
The 2027 Gordon Research Conference on Applied and Environmental Microbiology will be held in South Hadley, Massachusetts. Apply today to reserve your spot.
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Dmitri Petrov @petrovadmitri.bsky.social · 17/09/2026
We are searching for an Assistant Professor in Evolutionary Biology! Looking for colleagues asking fundamental questions relating to evolutionary processes and drivers of evolutionary patterns. If you think it is you, please do apply! And please repost. facultypositions.stanford.edu/jobs/assista...
facultypositions.stanford.edu
Assistant Professor in Evolutionary Biology - Stanford University, California, United States
The Department of Biology at Stanford University seeks applicants for a tenure-track Assistant Professor in Evolutionary Biology. A successful candidate will conduct research that asks fundamental que...
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Janani Durairaj (Jay) @ninjani.bsky.social · 28/08/2026
Some exciting news: I'm joining the University of Lausanne @unil.bsky.social @dbc-unil.bsky.social as an Assistant Professor next month 🎉 My group will work on context-aware deep learning for protein structure, interaction & design. Postdoc and PhD openings coming soon - keep an eye out!
compare, predict, design
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simon-alkema.bsky.social @simon-alkema.bsky.social · 17/09/2026
Releasing TOMATO, the TOMogram Annotation TOol! A lightweight Python GUI for going through hundreds of cryo-ET tomograms. Flag what's in each one, grade its quality, and end up with a table you can filter and act on. github.com/alkemasimon/...
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Archaea Biotech @archaeabiotech.bsky.social · 17/09/2026
Cryo-EM training week at the University of Exeter, hosted by the Daum Lab! 🧊 Theory & sample prep 🔬 Data processing & structure elucidation 🤝 Scientific exchange & team time Thanks to our hosts and everyone who made it a great week! #ARCTECH #CryoEM #Archaea #MSCA #Science
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Debnath Ghosal @debnathghosal.bsky.social · 16/09/2026
Very pleased to see our recent work on the evolution of complex eukaryotic cells from simple microbial ancestors is featured in this @nature.com News article. www.nature.com/articles/d41....
nature.com
These bizarre, much-coveted microbes are revealing the origins of complex life
Researchers are racing to culture a menagerie of exotic organisms and finding lots of surprises along the way.
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Reposted by Pilhofer Lab 🦠❄️🔬
Cascales Lab @cascaleslab.bsky.social · 11/09/2026
New paper in Current Biology @currentbiology.bsky.social 🦠 Why do bacteria ditch their T6SS weapon? E. coli keeps it vs susceptible rivals, but loses it when facing immune ones. Great work by @bobytaillefer.bsky.social & Jonas Desjardins! www.cell.com/current-biol...
cell.com
Experimental evolution reveals genetic routes for adaptive loss of the antibacterial type VI secretion system
Taillefer et al. show that a bacterial type VI secretion system is lost or attenuated during experimental evolution when it provides no competitive advantage but is maintained in the presence of susce...
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Hauke Hillen @haukehillen.bsky.social · 11/09/2026
Happy to share our new preprint on the structure of the human mitochondrial RNA degradosome, a central player in organellar RNA metabolism! Work led by graduate student @paulafprado.bsky.social in our group. www.biorxiv.org/content/10.6... 🧵 1/9
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Asaf Levy @asaflevylab.bsky.social · 13/09/2026
Nice experimental evolution work from @cascaleslab.bsky.social on the mechanisms by which T6SS is lost during evolution when present with T6SS-immune competing bacteria, compared to its maintenance when competitors are T6SS susceptible. www.cell.com/current-biol...
cell.com
Experimental evolution reveals genetic routes for adaptive loss of the antibacterial type VI secretion system
Taillefer et al. show that a bacterial type VI secretion system is lost or attenuated during experimental evolution when it provides no competitive advantage but is maintained in the presence of susceptible competitors. T6SS inactivation arises through diverse regulatory and structural mutations, revealing how ecological context shapes antibacterial weapon evolution.
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Basler Lab @basler-lab.bsky.social · 09/09/2026
In our new paper, we uncover how Burkholderia switches from antibacterial T6SS-1 to eukaryotic-targeting T6SS-5. Surprisingly, actively turning off T6SS-1 assembly prevents mitochondrial damage and apoptosis. Congrats @christy-chc.bsky.social and the team! link.springer.com/article/10.1...
link.springer.com
Regulation of Type VI Secretion Systems of Burkholderia during transition to intracellular lifestyle - EMBO Reports
Pathogenic bacteria tightly regulate gene expression in response to environmental cues. In Burkholderia species, the Type VI Secretion Systems (T6SS) mediate interbacterial competition and host cell i...
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EMBL @embl.org · 09/09/2026
Congratulations, Julia Mahamid! The EMBL scientist has been elected to Leopoldina, the German National Academy of Sciences, in recognition of her outstanding contributions to the field of structural cell biology. Learn more: www.embl.org/news/awards-...
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Caitie McCafferty @computingcaitie.bsky.social · 09/09/2026
I am looking for a lab manager for my group that I will be starting at the @fmiscience.bsky.social in February! We will use in situ structural biology to investigate dynamic ciliary processes. More info here —> bsky.app/profile/comp... #cilia #teamtomo #proteomics #protistsky
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Department of Biology, ETH Zurich @eth-dbiol.bsky.social · 10/09/2026
Dein Lieblingsfach im Gymnasium war Biochemie, und du möchtest dich auch im Studium weiter in dieses Fach vertiefen? Dann studiere Biologie/Molekulare Biowissenschaften an der ETH Zürich! Raoul erzählt dir, warum dieser Entscheid richtig war. youtube.com/shorts/gqGve...
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Helmholtz Institute Würzburg @helmholtz-hiri.bsky.social · 07/09/2026
Looking to start your own lab? 🧪 #HIRI & @uni-wuerzburg.de are recruiting two junior professors. 🚨 The application deadline is in two weeks. 🧵 More details in the thread below. 👇
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Ben Engel @cellarchlab.com · 08/09/2026
MemBrain v2 is on @natmethods.nature.com now? I never had a @biorxivpreprint.bsky.social with 164 citations before😅 ...Which is also to say: Thank you to the amazing #TeamTomo community, many of whom not only beta tested, but also contributed diverse training data. This one belongs to all of us! 🤗🔬🧪
BioRxiv FTW
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Ricardo D. Righetto @lifeonthewedge.bsky.social · 08/09/2026
A wild publishing ride to get here... but finally! (first bioRxiv: January 5th, 2024 🙃) Amazing work from @lorenzlamm.bsky.social and colleagues 🤗 Special thanks to the community who jumped early on the boat, sharing feedback and training data to make MemBrain v2 a reality 🙏🏽
nature.com
MemBrain v2: an end-to-end tool for the analysis of membranes in cryo-electron tomography - Nature Methods
MemBrain v2 enables streamlined analysis of membranes in cryo-electron tomography by providing user-friendly tools for membrane segmentation, membrane protein localization and spatial analysis, suppor...
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Lara Krüger @lara-kruger.bsky.social · 09/06/2026
📢 We are looking for a Junior Group Leader to join us at @institutcurie.bsky.social! Having joined the department just over a year ago, I can honestly say that it is an exceptionally stimulating and supportive scientific environment, filled with outstanding colleagues — all in the heart of Paris.
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Florian Wollweber @florianwollweber.bsky.social · 05/09/2026
Exciting news! Our @erc.europa.eu StG project ARCHAEAOLOGY got funded 🎉 We’ll combine cryoET/EM, ExM & spatial omics to explore the subcellular organization of Asgard archaea. Job ads coming soon! 🦠 ❄️ 🔬 #TeamTomo #ArchaeaSky #MicroSky
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