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Reposted by Cathy Spangler

cryoEM papers @cryoempapers.bsky.social · 24/09/2026

Acoustofluidic cryo-EM enables in situ particle manipulation for uniform, high-quality cryo-EM specimens pubmed.ncbi.nlm.nih.gov/42777058/ #cryoEM

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Cathy Spangler @cathyspangler.bsky.social · 22/09/2026

I'm excited to share our new preprint, where we show that fine-tuning gold nanoparticle conjugation to different Fab structural elements can produce powerful, rigidly coupled, labeling tools for in-situ cryo-ET. Take a look if you want to see an AuNP in an EM map!🔬🧠 www.biorxiv.org/content/10.6...

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Cathy Spangler @cathyspangler.bsky.social · 21/08/2026

Lab bonding at a Timbers game! (we made it on TV sitting behind the goal, if you squint 😂)

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Reposted by Cathy Spangler

Richard Held @rgsheld.bsky.social · 01/12/2025

I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell. authors.elsevier.com/a/1mA6h,LqAr...

authors.elsevier.com

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Cathy Spangler @cathyspangler.bsky.social · 24/11/2025

Out today, we can now multiplex receptor mapping at synapses thanks to a dimeric gold nanoparticle label optimized by Hoyoung that's distinguishable from monomers! Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...

pnas.org

PNAS

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

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Cathy Spangler @cathyspangler.bsky.social · 27/05/2025

I've been lucky to be able to work with Natalie on producing several new monoclonal antibodies using these methods, with great success! Check out our group's new protocols paper on how to generate mAbs for various membrane proteins - currentprotocols.onlinelibrary.wiley.com/doi/full/10....

currentprotocols.onlinelibrary.wiley.com

Generation of Conformation‐Specific Monoclonal Antibodies for Integral Membrane Proteins

Antibodies and their antigen-binding fragments, including fragment antigen-binding domains (Fabs) and single-chain variable fragments (scFvs), are extraordinary tools in all fields of biology, partic....

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Cathy Spangler @cathyspangler.bsky.social · 21/03/2025

So glad to see this out! Congrats!

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Cathy Spangler @cathyspangler.bsky.social · 04/12/2024

Gotta love finding soccer balls in tomograms ⚽ 😂

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Cathy Spangler @cathyspangler.bsky.social · 03/12/2024

Loving the holiday spirit at PNCC to start off December 🎄 + early Xmas gift getting to test out Tomo5!

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Cathy Spangler @cathyspangler.bsky.social · 24/11/2024

First Bluesky post! Our recent eLife paper is now an official version of record. This was my first project using #Cryo-ET and I'm definitely hooked - it's so fun to look at snapshots of synapses within brain tissue and see individual proteins. Excited to share more soon! doi.org/10.7554/eLif...

doi.org

Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses

By harnessing antibody fragments conjugated to small gold nanoparticles together with high pressure freezing, FIB/SEM milling and cryo-electron tomography, AMPA receptors were identified from mouse br...

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