Current genetic screens face a trade-off between scaling to large mutant libraries and measuring information-rich phenotypes. SPARCS automates laser microdissection to enable pooled forward genetic screens for complex image-based phenotypes at scale. By physically recovering individual mutants, SPARCS supports multimodal profiling of hits beyond genotyping, including via mass spectrometry-based proteomics.

Transposable elements mobilize within and occasionally between genomes, including from host to virus. We identified two insertions of the human BC200 noncoding RNA gene in the poxvirus molluscum conta...

In Metazoa, transposon expression is suppressed by the piRNA pathway, and disruption of this pathway leads to rampant transposon expression. However, it remains unclear whether increased transposon ex...

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Nature Reviews Genetics - In this Journal Club, Claudia Keller Valsecchi recalls two seminal studies by Csankovszki et al. and by Wutz and Jaenisch that defined X-chromosome inactivation as an...

An analytical pipeline called Iteratively Defined Lengths of Inaccessibility (IDLI) maps the genome-wide occupancy of a range of nucleosome types and shows that most nucleosomes exhibit programmed ‘di...

Abstract. Prokaryotes and eukaryotes use diverse strategies to cope with invading mobile genetic elements, including programmed DNA elimination (PDE). In t

Background The ability to deposit histone H3K27-trimethyl (me3) marks is essential for transcriptional repression by Polycomb Repressive Complex 2 (PRC2). This is largely attributed to Polycomb repres...

Abstract. The type I interferon (IFN) response is the main innate immune pathway against viruses in mammals. This pathway must be tightly regulated to prev

Milovanović et al. uncover a mechanism by which KZFPs restrict the activity of transposon-derived cis-regulatory elements to safeguard cardiomyocyte functionality. This work extends the classical TE-K...

Building an in vitro model of receptive endometrium allows investigation of human embryo implantation and early post-implantation development and recapitulates the earliest stages in establishing the ...

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Nature Cell Biology - The authors optimize an in vitro human epiblast model, which they utilize to show that early TGFβ family inhibition prevents epithelial identity, whereas it is...

Nature Cell Biology - The authors show that increased Xist RNA levels can induce de novo silencing of genes that normally escape X inactivation. SPEN depletion prevents the silencing of escape...

Interview with Cassandra Extavour, who studies the evolution of the genetic mechanisms employed during early animal embryogenesis to specify cell fate, development, and differentiation at Harvard Univ...