iiinova — where your digital self keeps evolving

Reposted by Mitosis Lab

Kelly Nguyen @kellythd-nguyen.bsky.social · 27/08/2026

Such an honor to receive the Francis Crick Medal from the Royal Society. As I always say, I am just very lucky to have worked with amazing people. Big shoutout to all past and present lab members, mentors, colleagues and collaborators for continuing inspiring me!

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Svetlana Dodonova @dodonova-sveta.bsky.social · 19/06/2026

Excited to share our new preprint! 🧬❄️ by brilliant @mdreimann.bsky.social and great collaborators! Using cryo-ET&EM, we reveal archaeal chromatin in a near-native state: variable-beads-on-a-string fibers shaped by growth phase and histone composition #ArchaeaSky www.biorxiv.org/content/10.6...

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cryoEM papers @cryoempapers.bsky.social · 18/06/2026

Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules pubmed.ncbi.nlm.nih.gov/42309992/ #cryoEM

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Gautam Dey @gautamdey.bsky.social · 09/06/2026

Science really takes a village. Thrilled to share this with my friend @dudinlab.bsky.social, and with our brilliant and fearless teams, our collaborators around the world and our mentors - in my case a special shoutout to Buzz Baum, Jan Ellenberg and @monicabettencourt.bsky.social. 1/2

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Utz Ermel @uermel.bsky.social · 12/06/2026

You can take a look at a demo tomogram acquired with the xLPP of apo-ferritin on the cryoET data portal here: cryoetdataportal.czscience.com/datasets/10496 Raw data is of course also available for download!

cryoetdataportal.czscience.com

Dataset

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Jamie Blaza @jnb-lab.bsky.social · 11/06/2026

Really important development in improving contrast in the painfully low contrast images we get out of a cryo-TEM

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Mitosis Lab @mitosislab.bsky.social · 12/06/2026

Jon Chen @jonchenjk.bsky.social and Claris Chong's review of recent in situ cryo-ET chromatin studies and our opinion on an important future genomic direction is now published in Current Opinions in Structural Biology. www.sciencedirect.com/science/arti...

Gallery of recent cryotomograms of chromatin inside eukaryotic cells.

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Marieke Oudelaar @mariekeoudelaar.bsky.social · 27/10/2025

Happy to share our latest publication, in which we show that the arrangement of nucleosomes around CTCF sites contributes to higher-order organisation of chromatin into TADs: www.embopress.org/doi/full/10....

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Kathleen Bachynski @bachynski.bsky.social · 24/09/2025

This is huge: “A drug that provides near-perfect protection against H.I.V. with shots just twice a year will be made available at $40 per patient annually in low- and middle-income countries, offering new hope… making lenacapavir a realistic choice in countries with constrained resources.”

nytimes.com

Philanthropies Strike a Promising Deal to Turn Back H.I.V.

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Oli Clarke @olibclarke.bsky.social · 19/09/2025

For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data. Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...). Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å

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cryoEM papers @cryoempapers.bsky.social · 19/09/2025

Correlative MS Imaging for cellular identification and analysis of in situ cryo-ET www.biorxiv.org/content/10.1101/2025.09.16.676641v1 #cryoEM

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Tanmay Bharat @tbharat-lab.bsky.social · 31/08/2025

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social www.biorxiv.org/cgi/content/... @mrclmb.bsky.social @wellcometrust.bsky.social

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American Society for Cell Biology @ascbiology.bsky.social · 18/08/2025

🏅 Congratulations to Sandra Schmid (@czbiohub) on receiving ASCB’s 2025 E.B. Wilson Medal! This award honors distinguished researchers for far-reaching contributions to cell biology over a lifetime in science—an achievement Sandra exemplifies. #ASCB #CellBiology

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Lennart Hilbert @lennarthilbert.bsky.social · 14/08/2025

Our first expansion microscopy results. Imaged today on "normal" Zeiss LSM 900, revealing chromatin and RNA polymerase II clusters at an unreal level of detail. Our PhD student @mofrawe.bsky.social learned the protocol from amazing @mpownall.bsky.social 🙏 Months of preparation are paying off now!

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Mitosis Lab @mitosislab.bsky.social · 14/08/2025

Looks like us structural cell biologists will have to be satisfied with false-atomic resolution for awhile.

A paper reports a new resolution regime: "true-atomic" resolution.

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Pilhofer Lab 🦠❄️🔬 @pilhoferlab.bsky.social · 12/08/2025

Check out 🧐 our new preprint 📜: www.biorxiv.org/content/10.1... Congrats🎉 to @vasilgaisin.bsky.social and Corina Hadjicharalambous, who studied contractile injection systems directly in hot spring♨️ bacterial mats🦠 #microsky #teamtomo #Chloroflexota

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Ahmad Jomaa @jomaalab.bsky.social · 01/08/2025

Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: www.biorxiv.org/content/10.1...

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Iain Cheeseman @iaincheeseman.bsky.social · 23/07/2025

New preprint! We solve a mystery you didn't know existed. Mitotic cells lack new transcription but require ongoing translation. Interphase mRNA half life is only 2-4 hrs. So how do cells arrest in mitosis for hours without depleting their transcriptomes? www.biorxiv.org/content/10.1...

biorxiv.org

Global inhibition of deadenylation stabilizes the transcriptome in mitotic cells

In the presence of cell division errors, mammalian cells can pause in mitosis for tens of hours with little to no transcription, while still requiring continued translation for viability. These unique...

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Michael Grange @michaelgrange.bsky.social · 21/07/2025

And here it is now in print! www.cell.com/cell-reports... With a front cover splash 😍

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Anders Sejr Hansen @andersshansen.bsky.social · 20/07/2025

This seems like a really important paper comparing chromosome tracing methods, demonstrating that classical denaturing DNA-FISH and chromosome tracing leads to substantial distortion of 3D genome structure, likely due to the high-temperatures involved (70-90C): www.nature.com/articles/s41...

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Stan Yatskevich @stanislauy.bsky.social · 15/07/2025

Happy to share our latest work on the structure and assembly of holocentric kinetochores! Huge thanks to Ines for a very fruitful collaboration, Claudio for all the support, and congratulations to Christine and all co-authors! www.biorxiv.org/content/10.1...

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eLife @elife.bsky.social · 12/07/2025

Frustrated with confusing or conflicting requests for revisions? At eLife, editors and reviewers discuss their reviews with each other before reaching a decision on a manuscript. Learn more about peer review at eLife: buff.ly/ioUIZyz

A visual diagram illustrating the steps in eLife's publishing process: 1. Submission, 2. Peer review, 3. Publication, 4. Author revision, and 5. Final version of record.

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Kelly Nguyen @kellythd-nguyen.bsky.social · 10/07/2025

Proud to share our work where we resolved a longstanding question regarding the existence of a human telomerase dimer and provided insights into its function. Led by 3 amazing lab members in collaboration with @yiliangding.bsky.social and @rdaslab.bsky.social. www.science.org/doi/10.1126/...

science.org

Cryo-EM structure of human telomerase dimer reveals H/ACA RNP-mediated dimerization

Telomerase ribonucleoprotein (RNP) synthesizes telomeric repeats at chromosome ends using a telomerase reverse transcriptase (TERT) and a telomerase RNA (hTR in humans). Previous structural work showe...

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Juha Huiskonen @juhahuiskonen.bsky.social · 08/07/2025

In our latest preprint on bioRxiv, we present the in situ structure of the human gap junction at 14 Å resolution using cryo-ET. The structure provides a blueprint for investigating how connexin regulation shapes intercellular communication in health and disease.

CryoET tomogram with the gap junction (green), ribosomes (cyan) and microtubules (pink)

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Pilhofer Lab 🦠❄️🔬 @pilhoferlab.bsky.social · 02/07/2025

Excited and honored to be joining the EMBO Member community 🥳 Congratulations also to all the other newly elected members! -Martin

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Dimitry Tegunov @dtegunov.bsky.social · 01/07/2025

Whoever gets the resolution of raw tomograms to <10 Å within the sampled parts of Fourier space would probably deserve a Nobel. Or they'd get one anyway for the crazy new physics they discovered to achieve that.

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Kelly Nguyen @kellythd-nguyen.bsky.social · 19/06/2025

Truly honoured to be awarded a Lister Prize this year. I am grateful for all the lab members and collaborators for their amazing work in the last nearly 6 years. More exciting science to come with this support.

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Paolo Swuec @pscryoem.bsky.social · 19/06/2025

Pit stop for our Aquilos dewar got even easier with the 3D printed funnel from friendly Zurich team (shout-out to blueskyless Piotr Szwedziak)!

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Paul & Virginie - Centriole Lab @centriolelab.bsky.social · 19/06/2025

🚨 New preprint! Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM www.biorxiv.org/content/10.1...

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · 18/06/2025

Our new review paper is out@JMB!🧬 @katsuminami.bsky.social‬ @semeigazin.bsky.social‬ ‪@kakonakazato.bsky.social‬ discuss DNA accessibility in euchromatin and heterochromatin of live cells. Free access: authors.elsevier.com/a/1lHgb54HFZ... Also check our previous work: www.science.org/doi/10.1126/...

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Michael Grange @michaelgrange.bsky.social · 17/06/2025

Proud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social

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Ahmad Jomaa @jomaalab.bsky.social · 16/06/2025

Check out our latest preprint with the Qi lab on the structure and disease physiology of the human ERAD complex. Outstanding work by Leo, a cell physiologist whom we successfully tempted into structural biology! Preprint: www.biorxiv.org/content/10.1...

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Misha Kudryashev @mishakudryashev.bsky.social · 08/06/2025

We have a preprint for you EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...

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MRC Laboratory of Molecular Biology @mrclmb.ac.uk · 05/06/2025

Congratulations to @tbharat-lab.bsky.social, Group Leader in the LMB’s Structural Studies Division, who has received the Gold Medal from @embo.org for his research on prokaryotic surface molecules. Read more here: www2.mrc-lmb.cam.ac.uk/tanmay-bhara... #LMBNews

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Svetlana Dodonova @dodonova-sveta.bsky.social · 26/05/2025

We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social! www.biorxiv.org/content/10.1... #Chromatin #Asgard #Archaea #cryoEM

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · 25/04/2025

🧬My retrospective chromatin review is out @Proc Jpn Acad: doi.org/10.2183/pjab... The textbook view of #chromatin as 30-nm fibers is fading. A new paradigm: chromatin forms #liquid-like domains—locally dynamic, yet globally stable at the chromosome level (viscoelastic)—supporting genome function. ✨

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Kelly Nguyen @kellythd-nguyen.bsky.social · 16/04/2025

We are looking for a postdoctoral fellow to join us to explore telomerase regulation by histones. Please get in touch if this may interest you.

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eltsovmikhail.bsky.social @eltsovmikhail.bsky.social · 17/04/2025

A question for the experts: Can you recognize nucleosomes and linker DNA in this data? Find an updated version of our manuscript in www.biorxiv.org/content/10.1...

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Sergio Cruz-León @sergiocruzleon.bsky.social · 11/04/2025

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social, @marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social 🔗 Preprint here tinyurl.com/3a74uanv

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Jeremy Baskin @jeremybaskin.bsky.social · 03/04/2025

Groundbreaking study in Cell from @leventallab.bsky.social: phospholipid asymmetry is a defining feature of the plasma membrane and cholesterol fills the holes — major implications for how this membrane works. A #lipidtime must-read! www.cell.com/cell/abstrac...

cell.com

Cell membranes sustain phospholipid imbalance via cholesterol asymmetry

This work challenges a major assumption in cell biology by showing that lipid bilayers can have drastically different phospholipid abundances between their two leaflets. This lipid abundance asymmetry...

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · 29/03/2025

Our new paper is out@ScienceAdvances👇 www.science.org/doi/10.1126/... 🧬Our Repli-Histo labeling marks nucleosomes in euchromatin and heterochromatin in live human cells. 🔍 @katsuminami.bsky.social et al. have developed a chromatin behavior atlas within the nucleus. 1/2

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