iiinova — where your digital self keeps evolving

Reposted by Lander Lab

Jan-Hannes Schaefer @jhschaef.bsky.social · 07/04/2026

We identified the maltoside detergent DM as an effective and easy solution to address preferred orientation in cryo-EM. Helped me switch from graphene grids. Special congrats to Maria Rafiq! @landerlab.bsky.social @nanigrotjahn.bsky.social @wisemanlab.bsky.social #cryoEM doi.org/10.64898/202...

Blender Rendering of vitrified HA trimer with DM at the air-water interface

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Reposted by Lander Lab

Jan-Hannes Schaefer @jhschaef.bsky.social · 05/10/2025

Helical Reconstruction of Amyloids in cryoSPARC? We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social. Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...

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Lander Lab @landerlab.bsky.social · 13/09/2025

So happy to see these developments! Particularly the structure of the 29 kDa heterodimer of hemoglobin! Well done Oli & co! Of course can’t overlook the importance of sample prep and data collection - credit to Mark Herzik and Mengyu Wu

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Reposted by Lander Lab

Jan-Hannes Schaefer @jhschaef.bsky.social · 01/08/2025

Check out our new preprint from the @landerlab.bsky.social, Stagg-Lab and Cianfrocco-Lab: CryoSift - An accessible and automated CNN-driven tool for cryo-EM 2D class selection www.biorxiv.org/content/10.1... Happy testing: cryosift.org #cryoEM #CNN

CryoSift – An accessible and automated CNN-driven
tool for cryo-EM 2D class selection

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Reposted by Lander Lab

Jan-Hannes Schaefer @jhschaef.bsky.social · 24/06/2025

Exited to share my first PostDoc contribution! Like Magellan explored the globe, you can explore your cryoEM data with Magellon. Congrats to all involved. Scott Stagg Lab & Michael Cianfrocco Lab @landerlab.bsky.social www.biorxiv.org/content/10.1... 🌎❄️🔬

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Reposted by Lander Lab

Danielle Grotjahn @nanigrotjahn.bsky.social · 01/05/2025

🚨New preprint!🚨 #teamtomo We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles. Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science

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Lander Lab @landerlab.bsky.social · 01/05/2025

Our latest review is out in COSB! Led by Zeba Rizvi @zrizvi.bsky.social,‬ we explore how cryo-EM has brought new insights to Cullin RING ligases - from conformations to allosteric control, cryo-EM has massively improved our understanding of these dynamic molecular machines. tinyurl.com/y4sza8dh

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Lander Lab @landerlab.bsky.social · 21/04/2025

Social media has been great in helping us identify contaminants or cofactors in the past - Any ideas what this complex might be in our HEK293 FLAG pulldown? (We should put together a repository of common contaminants in different cell types)

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Reposted by Lander Lab

Lauren Anderson @lauanderson.bsky.social · 23/03/2025

Finally recovered from 2 weeks of #cryoEM What a blast! After 3 times TA-ing for CSHL, I can’t begin to describe the joy it has brought me to teach and learn at this amazing course. Thanks to @jencash.bsky.social @landerlab.bsky.social @matthijnvos.bsky.social et al. for bringing this to life!

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Reposted by Lander Lab

Eric Topol @erictopol.bsky.social · 23/03/2025

A pie graph worth keeping in mind as the NIH budget plummets jamanetwork.com/journals/jam... for 356 new FDA drugs approved

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Reposted by Lander Lab

Jan-Hannes Schaefer @jhschaef.bsky.social · 22/03/2025

Read of the week. Is this future of freezing cryo-EM grids - Foam Film Vitrification without blotting? Congrats to Russo and Löwe labs!

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Lander Lab @landerlab.bsky.social · 21/03/2025

Big congrats to Dr. Kelly Chen on completing her PhD! She wrangled human ClpXP into submission with CryoEM, and now she's off to tackle new challenges at Genentech. We couldn’t be more proud of all she’s accomplished, and we all wish her all the best in this next chapter of her scientific journey.

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Lander Lab @landerlab.bsky.social · 19/03/2025

That’s a wrap on the 2025 Cold Spring Harbor CryoEM course! Huge thanks to the amazing students for your enthusiasm, the TAs for your dedication, and the invited lecturers for sharing your expertise. Keep pushing the boundaries of single particle EM - excited to see what you all accomplish next!

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Lander Lab @landerlab.bsky.social · 19/03/2025

Congrats to Group 1, winners of the 2025 CSHL #CryoEM course! They reconstructed a 1.9 A structure from 100 images, the built and refined an outstanding atomic model with great validation metrics. They are the recipients of the new Cryo Champion Chalice, adorned with $2,467 worth of used autogrids!

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Lander Lab @landerlab.bsky.social · 18/03/2025

CSHL 2025 #CryoEM course update - final hour of the competition and the teams are refining their atomic models. Amazing what these students have done with 100 micrographs in just a few hours!

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Lander Lab @landerlab.bsky.social · 18/03/2025

It’s the last full day of the CSHL #CryoEM course! Last leg of the competition - solve the structure of a mystery protein and generate an atomic model with a complete Table 1 (sequence not provided). Students are learning that ChatGPT isn’t much help when trying to identify symmetry of a structure…

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Lander Lab @landerlab.bsky.social · 18/03/2025

Brace yourselves, Matthijn Vos @matthijnvos.bsky.social has arrived! EM expert & head of the NanoImaging Core at the Institut Pasteur, he's known for his razor-sharp critiques and brutal honesty. But beneath the grumpiness? A brilliant scientist and an invaluable colleague. Welcome, Matthijn!

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Lander Lab @landerlab.bsky.social · 17/03/2025

Happy St. Patrick’s Day from the CSHL #cryoEM course! Data collection day for the final competition, but it looks like some mischievous leprechauns got into the microscope and messed up the autoloader… A tough but valuable lesson for the students: sometimes your resolution is limited by bad luck…

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Lander Lab @landerlab.bsky.social · 17/03/2025

Day 11 of the CSHL #CryoEM course: depositing data and new developments at the EMDB with Jack Turner, @jencash.bsky.social showed how to make graphene oxide grids, and learned how to make local resolution maps. Competitive data collection starts tomorrow!

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Lander Lab @landerlab.bsky.social · 16/03/2025

We’ve seen some good grids and some bad at the CSHL #CryoEM course, but I think we all agree that @miroastore.bsky.social should be awarded the 2025 “worst grid” award. But for someone who never held a pipette prior to this course he also deserves the “most improved” award - keep it up Miro!

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Lander Lab @landerlab.bsky.social · 16/03/2025

Saturday night and the lab is in full swing as the participants of the CSHL #CryoEM course prepare for the final competition - solve the structure of a mystery protein in less than 4 hours.

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Lander Lab @landerlab.bsky.social · 14/03/2025

Outstanding lecture on EM validation by Oli Clarke @olibclarke.bsky.social this morning at the CSHL #cryoEM course.

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Lander Lab @landerlab.bsky.social · 14/03/2025

Today at the CSHL #CryoEM course: we began with an excellent overview of atomic model validation by Christopher Williams, then an in-depth modeling tutorial by @jencash.bsky.social, a masking and local refinement tutorial by Kelli H, and tonight the students begin flying solo on the Krios!

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Reposted by Lander Lab

Alexey Amunts @amunts.bsky.social · 12/03/2025

Sad news about Karen Davies' passing. Karen was an inspiration in bioenergetics and cryoET. Her pioneering postdoctoral work on ATP synthase and respiratory supercomplexes marked turning points in the field. I created Scholar profile to acknowledge her contribution scholar.google.com/citations?hl...

scholar.google.com

Karen Davies

‪Formerly of Electron Bio-Imaging Centre, Diamond Light Source, United Kingdom‬ - ‪‪Cited by 2,887‬‬ - ‪cryo-EM‬ - ‪tomography‬ - ‪method development‬ - ‪bioenergetics‬

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Lander Lab @landerlab.bsky.social · 12/03/2025

Our first competition at the CSHL #CryoEM course - each student tries to make a perfect grid of ryanodine receptors. We’ll be checking atlases after dinner… who will be tonight’s big winner? Thanks to @olibclarke.bsky.social for providing sample and gold grids!

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Reposted by Lander Lab

Edward T Eng @edwardteng.bsky.social · 11/03/2025

Always here to be a resource for the #cryoEM community. It was great introducing what structural biology can do for science and how NYSBC as a service center can support the next generation of scientists. I look forward towards their research journey. Curious? Let us know more: cryoemcenters.org

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Lander Lab @landerlab.bsky.social · 11/03/2025

Day 6 of the CSHL #CryoEM course: field trip to the city! Visited the New York Structural Biology Center, where Ed Eng @edwardteng.bsky.social introduced everyone to the EM sample prep and collection resources available at nccat.nysbc.org. Then a tour of the new technologies being developed there!

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Reposted by Lander Lab

MRC Laboratory of Molecular Biology @mrclmb.ac.uk · 11/03/2025

Great #postdoc opportunity to join @annebertolotti.bsky.social’s group in the LMB’s Neurobiology Division, working on innovative approaches to rescue mice from protein misfolding in neurodegenerative disease. More info: www.nature.com/naturecareer... Apply by 03 APR #PostdocJobs #ScienceJobs

Anne Bertolotti, stood smiling in front of a lab bench

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Lander Lab @landerlab.bsky.social · 11/03/2025

Day 5 of the CSHL #cryoEM course: clipping and loading autogrids. Thanks to SubAngstrom for supplying us with rings and clips for the course! Kelli H also gave an amazing tutorial on 3D mask creation, which we put to use for a signal subtraction tutorial run tonight by Katerina Naydenova.

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Reposted by Lander Lab

Scripps Research @scripps.edu · 10/03/2025

A DNA repair enzyme that some cancers rely on to survive could be the key to more precise treatments. The @landerlab.bsky.social has captured the first images of Pol-theta in action, revealing how it repairs DNA—offering a blueprint for better cancer therapies.

ow.ly

How a crucial DNA repair protein works—and what it means for cancer treatment

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Lander Lab @landerlab.bsky.social · 09/03/2025

Day 4 of the CSHL #CryoEM course - A great lecture on advanced processing strategies from Justin Kollman, then more hands-on training! Freezing cryo samples with the Leica GP2, TFS Vitrobot, and manual plunger; preparing and checking negative stain grids; and checking alignments on the Krios.

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Lander Lab @landerlab.bsky.social · 08/03/2025

Everyone crowding around Matthijn Vos for an introductory demo on the Krios on day 3 of the CSHL #CryoEM course

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Reposted by Lander Lab

Micha Rapé Lab @micharapelab.bsky.social · 08/03/2025

Thank you to Christina Woo for presenting such a data-packed, standing-room only seminar in Molecular Therapeutics @ucberkeleyofficial.bsky.social this week! Awesome work!!!

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Lander Lab @landerlab.bsky.social · 08/03/2025

Today at the CSHL #CryoEM course Lauren @lalexandrescu.bsky.social showed everyone how to make their own Quantifoil-style holey cryo-em grids! Thanks to @johnrubinstein.bsky.social for introducing us to this technique - I predict it will be adopted by many other labs!

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Lander Lab @landerlab.bsky.social · 07/03/2025

We couldn't get out to #StandUpForScience with everyone else, but lots of great science discussed today at the CSHL #CryoEM course - intro to electron microscope optics with Alex de Marco, Fourier transforms & CTF with Tim Grant, and direct detectors with Katerina Naydenova.

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Lander Lab @landerlab.bsky.social · 07/03/2025

BTW welcome to Bluesky, Yifan Cheng @cryosp.bsky.social Professor at UCSF, HHMI investigator, #CryoEM pioneer, and aggregator of fish, chicken, and cannolis on a single plate.

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Lander Lab @landerlab.bsky.social · 07/03/2025

Amazing Day 1 of the CSHL #CryoEM Course! Yifan Cheng @cryosp.bsky.social kicked things off with a historical perspective and discussing dynamic allostery, Kelli Hvorecny gave a workflow overview, Lauren Anderson introduced ChimeraX @www.cgl.ucsf.edu, and Katerina Naydenova ran a RELION tutorial.

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Lander Lab @landerlab.bsky.social · 06/03/2025

Welcome to the participants of the 2025 #CryoEM course at Cold Spring Harbor Lab @cshlaboratory.bsky.social ! It's going to be a great 2 weeks immersed in all aspects of single particle cryoEM methods.

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Lander Lab @landerlab.bsky.social · 03/03/2025

Congrats to our postdoc Jan-Hannes Schaefer @jhschaef.bsky.social on being awarded a fellowship from the Walter Benjamin Program! Jan is collaborating with the Kelly lab here at Scripps Research @scripps.edu to understand how transthyretin forms amyloids that lead to heart disease.

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Lander Lab @landerlab.bsky.social · 03/03/2025

Congrats to Chris Zerio on publication of his study showing how cancer-associated Polymerase Theta undergoes both composition and conformational rearrangements to bring together broken strands of DNA for repair. Great collaboration with MOMA therapeutics. Now in peer-reviewed form: rdcu.be/ebFya

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Sjors Scheres @sjorsscheres.bsky.social · 27/01/2025

We have an exciting opportunity for a new independent group leader with an interest in structural/molecular biology and machine learning to join our Division of Structural Studies at the @mrclmb.bsky.social! 🥳 Re-posts appreciated. www.nature.com/naturecareer...

nature.com

Research Group Leader Tenure Track - Structural Studies - LMB 2549 - Cambridgeshire job with MRC Laboratory of Molecular Biology | 12835196

Research Group Leader Tenure Track Starting Salary £65,940 to £74,448 per annum MRC Laboratory of Molecular Biology, Cambridge, UK

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Lander Lab @landerlab.bsky.social · 22/01/2025

Just because it's a homo-oligomer, don't assume it's symmetric! @benjabsnt.bsky.social & collaborators used cryo-EM to study the transthyretin (TTR) tetramer (55 kDa), shedding light on its misfolding pathways linked to amyloid disease. Now in peer-reviewed form: www.nature.com/articles/s41...

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Lander Lab @landerlab.bsky.social · 16/01/2025

Protein production for cryoEM structure determination just got a lot cheaper - high resolution structure from half a plate of HEK cells with the "MIcro ISolation (MISO)" microfluidics-based approach. Congrats to the Brunner and Efremov labs in Brussels! www.biorxiv.org/content/10.1...

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Janet Iwasa @jiwasa.bsky.social · 07/01/2025

I'm happy and humbled to take on a new role with @rcsbpdb.bsky.social. I'll be continuing David Goodsell's work on the Molecule of the Month, starting with my first article on Assembly Line Polyketide Synthases! Check it out (animation included!) pdb101.rcsb.org/motm/301

pdb101.rcsb.org

PDB101: Molecule of the Month: Assembly Line Polyketide Synthases

Large multienzyme complexes that synthesize diverse small molecules in a stepwise manner.

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RCSB Protein Data Bank @rcsbpdb.bsky.social · 06/01/2025

After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month. We are very grateful for his service. @jiwasa.bsky.social will continue the series, beginning with January's article on Assembly Line Polyketide Synthases Details: cdn.rcsb.org/rcsb-pd...

cdn.rcsb.org

EDUCATION CORNER: Molecule of the Month: Celebrating 25 Years of Storytelling and Announcing New Beginnings

After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month series. Janet Iwasa will be continuing the series for PDB-101.

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Lander Lab @landerlab.bsky.social · 07/01/2025

Last chance to apply to the Cold Spring Harbor #cryoem course this March (application deadline is Jan 15th)! Learn all about sample prep, microscope operation, and image processing at this fun 2-week course @jencash.bsky.social @cshlnews.bsky.social meetings.cshl.edu/courses.aspx...

meetings.cshl.edu

Cryo-Electron Microscopy

Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

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Keren Lasker @kerenlasker.bsky.social · 30/12/2024

1/ 🚨We’re thrilled to share our latest study: "Cellular Function of a Biomolecular Condensate Is Determined by Its Ultrastructure" 🌟 www.biorxiv.org/content/10.1... How do biomolecular condensates achieve their cellular roles? It comes down to their internal structure. 🧵⬇️

biorxiv.org

Cellular Function of a Biomolecular Condensate Is Determined by Its Ultrastructure

Biomolecular condensates play key roles in the spatiotemporal regulation of cellular processes. Yet, the relationship between atomic features and condensate function remains poorly understood. We stud...

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Lander Lab @landerlab.bsky.social · 19/12/2024

A huge week for Prakhyat, our joint @scripps.edu student with the @wisemanlab.bsky.social! He was awarded an AHA earlier in the week and today he passed his PhD candidacy exam! Because Prakh is so chill, we celebrated with chocolate-coffee liquid nitrogen ice cream. 😎

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