Reposted by @abhaykot.bsky.socialJess Heebner @jessheebner.bsky.social · 09/01/2026I'm so happy to have gotten the opportunity to work on this project and I'm SO excited that they selected our image for the cover! 0147
Reposted by @abhaykot.bsky.socialBen Engel @cellarchlab.com · 09/01/2026The #ChlamyDataset is on the cover of @cp-molcell.bsky.social 🖼️🥰! Read @lifeonthewedge.bsky.social's great thread🧵 for the inside scoop🍨 on all the #TeamTomo developments already made possible since these 1829 tomos hit EMPIAR 🧪 🧶🧬 For more, here's the old preprint thread: bsky.app/profile/cell... 03510
Reposted by @abhaykot.bsky.socialRicardo D. Righetto @lifeonthewedge.bsky.social · 09/01/2026This one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell! This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍 [Maybe long thread ahead]cell.comToward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtiiUsing the latest advances in instrumentation and computational workflows, Kelley et al. present a large-scale annotated cryo-electron tomography dataset of the model green alga, Chlamydomonas reinhard... 39736
Reposted by @abhaykot.bsky.socialMolecular Cell @cp-molcell.bsky.social · 08/01/2026The latest issue is now online www.cell.com/molecular-ce... 0197
Reposted by @abhaykot.bsky.socialPhilippe Van der Stappen @phaips.vd.st · 06/05/2025Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪tomoguide.github.ioWelcome to TomoGuideA step-by-step Cryo-ET guide 512344
Reposted by @abhaykot.bsky.socialScience Magazine @science.org · 20/03/2025In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion. Learn more in this week's issue: scim.ag/3FA3Ygq 15464144
Reposted by @abhaykot.bsky.socialRicardo D. Righetto @lifeonthewedge.bsky.social · 28/02/2025To all #TeamTomo #CryoET and #Chlamydomonas aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽💻 www.ebi.ac.uk/empiar/EMPIA... A little thread about what's new... 🧵 1/n 26422
Reposted by @abhaykot.bsky.socialAlicia Michael @aliciakmichael.bsky.social · 06/01/2025I am happy to be a part of this effort and looking forward to the discoveries this dataset will enable! If you work with this dataset in the future, please add any annotations to the GitHub repository github.com/Chromatin-St... Thanks @paloha.bsky.social for spearheading the efforts with this!github.comGitHub - Chromatin-Structure-Rhythms-Lab/ChlamyAnnotations: Particle annotations for the large-scale cryo-ET dataset of Chlamydomonas reinhardtiiParticle annotations for the large-scale cryo-ET dataset of Chlamydomonas reinhardtii - Chromatin-Structure-Rhythms-Lab/ChlamyAnnotations 1133
Reposted by @abhaykot.bsky.socialBen Engel @cellarchlab.com · 06/01/2025This large dataset is the result of years of collaborative #TeamTomo work between @abhaykot.bsky.social's TFS team, our @biozentrum.bsky.social group, and the @mpibiochem.bsky.social crew led by John and Jürgen. Abhay's team used automated plasma #FIB milling and 42 overnight #cryoET sessions! 🧪🔬💪 1101
Reposted by @abhaykot.bsky.socialBen Engel @cellarchlab.com · 06/01/2025Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍 Preprint📜: www.biorxiv.org/content/10.1... A short thread🧵👇 7338128
Reposted by @abhaykot.bsky.socialPhilippe Van der Stappen @phaips.vd.st · 29/12/2024More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy! Check out the preprint: www.biorxiv.org/content/10.1... Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com #TeamTomobiorxiv.orgTowards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtiiIn situ cryo-electron tomography (cryo-ET) has emerged as the method of choice to investigate structures of biomolecules in their native context. However, challenges remain in the efficient production... 38535
Reposted by @abhaykot.bsky.socialMark Peifer (He, him) @peiferlabunc.bsky.social · 29/12/2024WOW! "Subtomogram averaging Soluble and membrane-bound complexes ranging in size from >3 MDa to ~200 kDa, including 80S ribosomes, Rubisco, nucleosomes, microtubules, clathrin, photosystem II, and mitochondrial ATP synthase. The majority of these density maps reached sub-nanometer resolution..." 🧪 45019
Reposted by @abhaykot.bsky.socialBasil Greber @bjgreber.bsky.social · 17/02/2024Our first "lab paper" is out: In collaboration with Sandro Ataide and Yichen Zhong (University of Sydney) and Adrian Koh and @abhaykot.bsky.social (TFS), Junjie Feng in my lab determined the structure of the peptide binding domain of SRP68/72, the largest protein components of the eukaryotic SRP. 4208
Reposted by @abhaykot.bsky.socialErvin Fodor @efodor.bsky.social · 07/11/2023A fantastic postdoc opportunity with Jon Grimes at Strubi, University of Oxford, to study the molecular mechanisms of viruses using the latest cryo-ET and cryo-SIM technologies. my.corehr.com/pls/uoxrecru...my.corehr.comJob Details 078
Reposted by @abhaykot.bsky.socialBasil Greber @bjgreber.bsky.social · 27/10/2023This was missed due to major live events happening around the same time, but we have released some of our high-resolution (2 Å) and rapid screening #cryoEM maps & models of the human CAK bound to small molecules. Paper still under review - more to follow. www.ebi.ac.uk/emdb/search/... 0206
