dynaMIET enables simultaneous measurement of lateral diffusion and vertical membrane fluctuations in live cells.

Reaction-based sensing is a rapidly expanding principle in the design of fluorescent probes. Although effective for sensing highly reactive species, it is less suitable for more complex small molecule...

Banerjee, Anand, and colleagues present an expanded speed-optimized DNA-PAINT sequence repertoire. This repertoire allows for improved multiplexing capability in super-resolution imaging, maintaining ...

A modular platform enables the generation of fluorescent metal ion indicators with tunable emission colors and precise subcellular localization. Conjugation to self-labeling proteins yields bright, c...

A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

CenSpark is a dual-ligand fluorescent probe binding simultaneously inner and outer microtubule sites, a configuration unique to doublet and triplet microtubules, enabling selective live imaging of cen...

Nature Communications - Lin and colleagues present high-affinity split-HaloTag pairs for protein tagging and multiplexed labelling. This versatile system allows protein visualisation with diverse...

Fluctuating environments can lead to phenotypic heterogeneity within a monoclonal bacterial population, especially in response to antibiotics or the human immune system. Methods are required to analyz...

The microtubule cytoskeleton is the backbone of neuronal morphogenesis, driving the development of the dendrites and axon, and supporting trafficking to distant compartments. How neuronal microtubules...

Rhodamine dyes conjugated to targeting ligands can yield exceptionally bright fluorescent probes for live-cell imaging. However, the limited permeability of such rhodamine derivatives restricts their ...

A new version of TrackMate with improved integration of key AI algorithms and track edition improvements -

4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.

Enzyme-based self-labeling tags enable the covalent attachment of synthetic molecules to proteins inside living cells. A frontier of this field is ...

Live-cell imaging of mRNA encoding secretome proteins and translated nascent peptide markers show that secretome translation occurs at endoplasmic reticulum junctions near lysosomes, requires lun...

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

This Review provides insights into nanosensor technologies for monitoring cellular biomarkers, proposing a spatial framework to clarify their distinct advantages, challenges and performance for high-r...

Fluorescence and photoacoustic imaging are complementary modalities that provide distinct advantages for biological imaging: fluorescence microscopy offers high sensitivity and resolution, while photo...

Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....

Supervisors who invest in positive mentoring relationships with their PhD candidates also reap the benefits for their own research.

The Reisenbauer Lab is a research group in biocatalysis and enzyme engineering at the IST Austria.

GR-SCHUHMACHER

By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...

We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...