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β-Arrestins, multifunctional adaptor proteins that regulate G-protein-coupled receptors (GPCRs), form phase-separated condensates, suggesting that β-arrestin liquid–liquid phase separation organizes and diversifies GPCR signalling functions.

Two of our recent findings on condensates were posted on bioRxiv! In the first one, Huan made a surprising discovery that the excitation of fluorescently tagged proteins leads to condensate-mediated …...

dynaMIET enables simultaneous measurement of lateral diffusion and vertical membrane fluctuations in live cells.

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The functional complexity of G protein-coupled receptors (GPCRs) arises from their structural dynamics, which span timescales from nanoseconds to minutes. Single-molecule Förster Resonance Energy Tran...

Arrestin proteins bind active G protein-coupled receptors (GPCRs) through coordinated protein-protein, protein-phosphate, and protein-lipid interactions to attenuate G protein signaling and promote GP...

Nature Communications - The association and dissociation dynamics of the ECF transporter complex for vitamin B12 are visualized by single-molecule FRET, highlighting the original transport...

Nature Communications - Membrane lipid packing, influenced by cholesterol, lipid chain length, and saturation, regulates the affinity of biomolecular condensates, enhancing their interactions with...

Molecular-level interactions among lipids, cholesterol, and water dictate the nanoscale membrane organization of lipid bilayers into liquid-ordered (Lo) and liquid-disordered (Ld) phases, characterize...

Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...

A study describes the design of de novo ligand-binding transmembrane proteins, demonstrating their specific binding and activation of fluorogenic ligands.

Molecular plasma membrane organization and dynamics play an important role in cellular signalling. Advances in our understanding of the nanoscale architecture of the plasma membrane heavily rely on th...

This protocol presents a method based on fluorescence correlation spectroscopy for tracking the diffusive route of fluorescent molecules in a live cell.

The existence of the phenomenon of enhanced enzyme diffusion (EED) has been a topic of debate in recent literature. One proposed mechanism to explain the origin of EED is oligomeric enzyme dissociation. We used mass photometry (MP), a label-free single-molecule technique, to investigate the dependence of the oligomeric states of several enzymes on their ligands. The studied enzymes of interest are catalase, aldolase, alkaline phosphatase, and vanillyl-alcohol oxidase (VAO). We compared the ratios of oligomeric states in the presence and absence of the substrate as well as different substrate and inhibitor concentrations. Catalase and aldolase were found to dissociate into smaller oligomers in the presence of their substrates, independently of inhibition, while for alkaline phosphatase and VAO, different behaviors were observed. Thus, we have identified a possible mechanism which explains the previously observed diffusion enhancement in vitro. This enhancement may occur due to the dissociation of oligomers through ligand binding.

Single particle profiling (SPP) is a unique methodology to study nanoscale bioparticles such as liposomes, lipid nanoparticles, extracellular vesicles, and lipoproteins in a single particle and high t...

APPLICATION CLOSING DATE:  March 12th, 2025Human Technopole (HT) is an interdisciplinary life science research institute, created and supported by the Italian Government, with the aim of developing in