iiinova — where your digital self keeps evolving

Johannes Hohlbein @hohlbeinlab.bsky.social · 18/09/2026

How is it going? Problems with prototype autofocus/focus stabilization performance => ask microscopy company to demo their microscope body with (perfect) focus stabilization => focus stabilization unit apparently currently broken awaiting repair... Kafka is calling.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 13/09/2026

(Cross post LinekdIn) V0.12.1 of #webSMLM released with inbuilt terminal functionality and experimental #smFRET (ALEX-TIRF) implementation.

GUI web interface of #webSMLM showing time traces and E/S histograms of smFRET data

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Christoph Dellago @chhdellago.bsky.social · 07/09/2026

If you are like me, you start every morning by making yourself a coffee with a moka pot. ☕️ While you wait for the coffee to bubble up, you can now explore how it works: compphys.univie.ac.at/Moka I made this (thank you, Claude!) for my thermo and stat phys course, but perhaps you enjoy it too.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 05/09/2026

(LinkedIn cross post) 0.12.0 of #webSMLM released. Work mainly by @kjamartens.bsky.social introduced live streaming and real time analysis from frames recorded in MicroManager or Python. hohlbeinlab.github.io/webSMLM/webS...

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Johannes Hohlbein @hohlbeinlab.bsky.social · 21/08/2026

(LinkedIn cross post) 0.11.3 of #webSMLM released. Since 0.11.0 we added: headless spectral SMLM runs, *.ND2 loading, single particle tracking (sptPALM, Nile red PAINT, etc), light (see below), dark or high contrast mode; *.svg export of plots, change to MIT license,... (1/2)

User interface of webSMLM, center window histogram of diffusion coefficients of Nile Red diffusing in bacterial cell wall, right window bacterial cell outlines of L. actis stained with Nile Red.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 17/08/2026

Update: #webSMLM (V0.11.0), a browser-based single-molecule localisation microscopy analysis tool, includes 2D/3D phasor/MLE fit, background correction, simulations, 3D drift correction, fast temporal median filtering, rendering, spectrally resolved SMLM (!),.. hohlbeinlab.github.io/webSMLM/webS...

Pic shows STORM data of fixated Cos7 cells with CF660-labeled clathrin and CF680-labeled tubules (cells: Lorenzo Albertazzi's lab). Colour coding represents emission wavelength dependent distance between 0th and 1st order.

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Glen Dsouza @nonresidentdesi.bsky.social · 10/08/2026

📣 We’re hiring a postdoc for @hot-mes-asu.bsky.social ! 🦠🔬 We’re looking for someone excited about microbial interactions, ecology & evolution, and using molecular genetics and microfluidics to understand microbial communities. Come do fun science with us! apply.interfolio.com/190859

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Johannes Hohlbein @hohlbeinlab.bsky.social · 20/07/2026

Want to analyse SMLM data on your phone (or any other browser)? Here we go: hohlbeinlab.github.io/webSMLM/webS..., repo on GitHub #webSMLM

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Luke Lavis @rhodamine110.bsky.social · 17/07/2026

Excited to see this collaboration now in print. Dye chemistry meets AI-designed proteins. Three small, orthogonal tags; lifetime modulation; split versions. Request the free dyes at dyes.janelia.org www.science.org/doi/10.1126/...

science.org

De novo design of orthogonal far-red, orange, and green fluorophore-binding proteins for multiplexed imaging

Fluorescent proteins and small-molecule dyes offer complementary advantages for biological imaging: proteins are amenable to genetic tagging, whereas dyes provide superior brightness and photostabilit...

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Dr Dan Jones @hookean.bsky.social · 10/07/2026

Here is the Physics Worksheet Generator. It has taken some time to get to this stage, but it currently covers GCSE and A-Level for most exam boards. Feedback welcome for errors or improvements. I will continue to work on it. I hope you find it useful #iTeachPhysics www.drjonesphysics.com/pwg

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Johannes Hohlbein @hohlbeinlab.bsky.social · 10/07/2026

Nothing fancy, just some old sample doing things earlier today. #FlourescenceFriday

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Cees Dekker @ceesdekker.bsky.social · 16/06/2026

WORK: our Bionanoscience department at TU Delft is looking to hire an Assistant Professor in Biophysics of Living Systems. Apply! Deadline 30 July careers.tudelft.nl/job/Delft-As... RT=nice

careers.tudelft.nl

Assistant Professor in Biophysics of Living Systems

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Dr Dan Jones @hookean.bsky.social · 27/06/2026

I have created over 100 free apps and simulations covering all major GCSE and A-Level Physics topics. Explore them here: #iTeachPhysics #Science 🧪 www.drjonesphysics.com/apps

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Johannes Hohlbein @hohlbeinlab.bsky.social · 19/06/2026

You might have heard about our GitHub repository on #OpenMicroscopy. I finally vibecoded a page that (a) allows to search all entries and (b) generates a new database as soon as items are added on GitHub: hohlbeinlab.github.io/OpenMicrosco... Check it out and comments welcome!

hohlbeinlab.github.io

Open Microscopy — Resource Index

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Jens Schmidt @jenscs83.bsky.social · 11/06/2026

I am looking to recruit 2 postdoctoral fellows to the lab to work on a recently funded project focused on class switch recombination in B cells. If you are interested in immunology, gene expression, and RNA and protein single molecule imaging, please reach out (schmi706@msu.edu). Please share!

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Naomi Chrispijn-Steenbeek @naomichrispijn.bsky.social · 08/06/2026

Join the NWO Physics of Life Seminar on 19 June at Utrecht Science Park Talks by Anna Erzberger (EMBL), Christian Kaiser (UU), Felix Frey (TU/e), Justin Tauber (WUR) Iris Smokers (MPI-CBG) Register <15 June shorturl.at/0VGb7 Organized by @floberger.bsky.social and @uddaloksen.bsky.social

shorturl.at

NWO Physics of Life seminar | NWO

The NWO physics of life research community committee welcomes you for a new seminar on June 19, 2026, at Utrecht Science Park. The seminar has an international keynote speaker, dr. Anna Erzberger (EMB...

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Johannes Hohlbein @hohlbeinlab.bsky.social · 19/05/2026

Ordering stuff for the lab is fun. Now I have white board markers but no white board and, even better, two new displays yet the delivery of the PCs got delayed to September 2026... Uff.

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Lorenzo Olivi @loreoliv.bsky.social · 11/05/2026

🧵 New pre-print out from my time at @mib-wur.bsky.social showing how the titration of the initiator protein DnaA on the chromosome of E. coli can generate stable DNA replication @hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social doi.org/10.64898/202...

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Johannes Hohlbein @hohlbeinlab.bsky.social · 07/05/2026

The "Witch of Agnesi" is not what I thought it would be. According to Wikipedia: a cubic plane curve defined from two diametrically opposite points of a circle.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 04/05/2026

Rough scaffolding of setup #2 done at Brandenburgische Technische Universität Cottbus-Senftenberg. Can actually test here the earlier reported effective vibration isolation for #SMLM using a bicycle inner tube. (Cross post LinkedIn)

Custom microscopy build using a ASI Ramm scaffolding system.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 25/04/2026

Moved some microscopy equipment last week to the new lab. Happy building. (Cross post LinkedIn)

Trunk of a car full of microscopy equipment.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 17/04/2026

Giving a lecture at Friday 8am is wild. Let’s see.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 04/04/2026

In academia this weird age is between the age of 25 and 55. At least. Am I right?

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Johannes Hohlbein @hohlbeinlab.bsky.social · 22/03/2026

Quick career update. Since beginning of March, I am appointed as Professor of Biosensorics at the Brandenburg University of Technology (BTU, Germany). Expect more single-molecule and single-cell inspired stuff & #OpenMicroscopy soonTM (🧬,🧫,🔬,🧪).

Blue sky at dusk overlooking the marina of lake Senftenberg close to the university. At this time of the year still without boats.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 08/03/2026

Was about to build a new PC for SMLM detection. Memory is ~500 Euros for 32Gb?!?!?! What?

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Johannes Hohlbein @hohlbeinlab.bsky.social · 03/03/2026

Guess we are living in the future now. Just submitted a manuscript and the website retrieved most information (abstract, names, etc) from the submitted files. I did not have to type everything again! 🤯 (disclaimer: keywords and authors’ contributions were not automatically detected...)

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Dolf Weijers @dolfweijers.bsky.social · 03/03/2026

We are very excited to share a new resource from our team: spatial subcellular proteome maps in plants! We developed an MS-based method that registers localizations of about 8000 proteins in Arabidopsis roots in a single experiment. (1/9) www.biorxiv.org/cgi/content/...

biorxiv.org

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Johannes Hohlbein @hohlbeinlab.bsky.social · 26/02/2026

Coordinated call for applications for three (!) thematically related professorships (cell physiology, molecular genetics, and biochemistry) at BTU Cottbus-Senftenberg (Germany) (thread)

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Jean-Yves Tinevez @jytinevez.bsky.social · 09/02/2026

New preprint alert! We developed an automated framework to optimize tracking pipelines for bacteria, along with a nice integration of tools for bacteria imaging, in the recently released TrackMate 8 (Fiji plugin). www.biorxiv.org/content/10.6... 👇🧵

biorxiv.org

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Dr Dan Jones @hookean.bsky.social · 02/02/2026

I have created an app to help students learn about emission and absorption spectra. Here is the link. I hope you find it useful 🌟 #iTeachPhysics #Science 🧪 #Astronomy 🔭 sites.google.com/view/hookean...

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Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 02/02/2026

iCASE PhD project available in my lab, combining microscopy method development with high throughput screening of bacteria. Applications to antibiotic drug discovery and screening for new cell division proteins. Collaboration with Cairn Research #microsky #bioimaging www.findaphd.com/phds/project...

findaphd.com

Image-based bacterial phenotyping and antimicrobial drug discovery using smart high-throughput super-resolution microscopy at University of Warwick on FindAPhD.com

PhD Project - Image-based bacterial phenotyping and antimicrobial drug discovery using smart high-throughput super-resolution microscopy at University of Warwick, listed on FindAPhD.com

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Achilles Kapanidis @achilleskap.bsky.social · 30/01/2026

Great to see our Science Review on emerging multiplexed single-molecule methods that use libraries to link sequence to dynamics. A fun collaborative writing project! From sequence to function: Bridging single-molecule kinetics and molecular diversity | Science www.science.org/doi/10.1126/...

science.org

From sequence to function: Bridging single-molecule kinetics and molecular diversity

Biological function is fundamentally determined by nucleic acid and protein sequence. Beyond encoding genetic information, nucleic acids also display complex physicochemical parameters that shape stru...

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Johannes Hohlbein @hohlbeinlab.bsky.social · 26/01/2026

Good resource for everyone interested in objectives and microscopy 👇

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Johannes Hohlbein @hohlbeinlab.bsky.social · 25/01/2026

@healthcare.nikon.com or others: Where can I find information on the field number of Nikon objectives? Seems missing from the objective comparison site? Specifically wondering about the CFI Apochromat TIRF 60XC Oil.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 19/01/2026

First rule when receiving a unsolicited chain mail at uni: REPLY ALL and say that you want to be removed from the list...

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Kyle M. Douglass @kmdouglass.bsky.social · 13/01/2026

I finally got around to writing the discussion section of our lab's basic training course on live cell #microscopy. I explain the tradeoffs involved in designing an imaging experiment and the art of thinking of the experiment as an optimization problem. leb-epfl.github.io/basic_traini...

leb-epfl.github.io

Introduction - Microscopy Basic Training

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Jason Rasgon @vectorgen.bsky.social · 01/08/2023

I guess I should re-post from the Bird Site "The Rules For Academic Success here": I always get asked for advice about starting/surviving the tenure track. Here are the rules I've found useful. Thought I'd put it out there for the masses... This list gets longer all the time

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Dina Grohmann @curiousdina.bsky.social · 05/01/2026

A new year brings new opportunities: we are looking to fill a permanent professorship position with a Microbiologist preferably with expertise on archaeal biology, RNA biology or imaging techniques to unravel cell biology in prokaryotes! Please share and/or apply :)! www.nature.com/naturecareer...

nature.com

Professurship (W2) in Microbiology - Regensburg (Stadt), Bayern (DE) job with Universität Regensburg | 12851113

UNIVERSITY OF REGENSBURG The Faculty of Biology and Pre-Clinical Medicine invites applications for a Professurship (W2) in Microbiology To be...

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Daniel Gorelick @danielgorelick.bsky.social · 31/12/2025

2025 was the year we stopped debating whether paying peer reviewers might work and started showing that it does.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 02/01/2026

I vaguely remember seeing a discussion about lab-onboarding etc on Twitter many years ago. This initiative below and the handbook look really valuable. Will implement.

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Johannes Hohlbein @hohlbeinlab.bsky.social · 01/01/2026

“Coffee doesn't talk, coffee doesn't whine, coffee just does its job. I like coffee.” Happy new year!

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Johannes Hohlbein @hohlbeinlab.bsky.social · 24/12/2025

Perspective is everything in life.

Picture of Halle (Saale) with a giant Christmas pyramid, the Market Church and the Red Tower. Somewhere hidden between Christmas market stands a statue of Händel can be seen.

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Achilles Kapanidis @achilleskap.bsky.social · 05/12/2025

New preprint! We share a new *DNA-only* method for single-molecule DNA sequencing, and use this cool tool to link single-molecule reactions/interactions to sequence. Big thanks🙏 to Jagadish Hazra @becs-andrews.bsky.social and all contributors since our 2016 start! www.biorxiv.org/content/10.1...

biorxiv.org

Unraveling single-molecule reactions via multiplexed in-situ DNA sequencing

DNA sequence regulates complex reactions and protein-DNA interactions, yet sequence effects remain poorly understood due to the lack of direct, high-throughput approaches to study sequence-dependence ...

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