MemBrain v2 enables streamlined analysis of membranes in cryo-electron tomography by providing user-friendly tools for membrane segmentation, membrane protein localization and spatial analysis, suppor...

In Drosophila, cell fate switching in adult intestinal stem cells is determined through an epigenetic mechanism in which these cells count divisions, switching from producing enterocytes to producing an enteroendocrine mother cell every ninth division.

Preferential orientation at the air-water interface (AWI) remains a persistent challenge in cryo-electron microscopy, often requiring extensive optimization or specialized grid preparation strategies....

Integrin adhesions allow dynamic cell-matrix interactions to regulate cell behaviour. Here, the authors reveal that tensin 1, a multidomain adaptor protein, forms condensates in cells that act as reve...

Protein domains: structure, classification, and evolution - An introductory tutorial for protein domains

eBIC (Diamond light source) has partnered with the Astbury Biostructure Lab (University of Leeds), the Scottish Centre for Macromolecular Imaging (University of Glasgow), Midlands Regional Cryo-EM Fac...

The ARP2/3 complex works together with actomyosin in an oscillatory program at the soma to govern neuronal polarization.

Paraschiakos, Yuan et al., using a computational pipeline, identify the short linear actin filament-binding motif (SFM) as an evolutionarily conserved actin filament-binding motif present in 103 human...

When stimulated by nucleation-promoting factors such as WAVE, the Arp2/3 complex generates branched actin networks. In contrast, when activated by SPIN90, the Arp2/3 complex generates linear actin fil...

Cryo-electron tomography (cryo-ET) visualizes 3D cellular architecture in near-native states. Recent deep-learning methods (CryoCARE, IsoNet, DeepDeWedge, CryoLithe) improve denoising and artifact cor...

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Human zyxin is a key component of the focal adhesion complex, playing a role in mediating cell-cell adhesion and cytoskeletal dynamics. Moreover, zyxin shuttles between the cytoplasm and the nucleus, ...

Huang et al. demonstrate that the intermediate filaments protein vimentin orchestrates actin stress fibers and podosomes, thereby facilitating efficient extracellular matrix (ECM) degradation via inte...

A step-by-step Cryo-ET guide

Scripts to make cryo-electron tomography a bit easier - tomotools/tomotools

Cryo electron microscopy (cryo EM) is a major structural biology method for studying macromolecular complexes and cellular structures in their native states. Stable high resolution cryo microscopes, …

The cytoskeleton is crucial for cell organization and movement. In Eukaryotes, it largely consists of the protein actin, that forms a double-strand...

‪Formerly of Electron Bio-Imaging Centre, Diamond Light Source, United Kingdom‬ - ‪‪Cited by 2,887‬‬ - ‪cryo-EM‬ - ‪tomography‬ - ‪method development‬ - ‪bioenergetics‬

eBIC (Diamond light source) has partnered with the Astbury Biostructure Lab (University of Leeds), the Scottish Centre for Macromolecular Imaging (University of Glasgow), Midlands Regional Cryo-EM Fac...