The authors develop SPIN-Seq, a method that directly links the sequence of individual DNA molecules to their functional behavior, revealing how DNA sequence impacts protein-DNA interactions and the ki...

Join us at the Keystone Symposia on DNA Replication: Pathway Crosstalk Shaping Physiology and Disease, April 2027, in London, with field leaders!

Cohesin-mediated loop extrusion limits sister-fork coupling and tethers nearby replication forks under replication stress, promoting fork reversal and slowing fork progression to safeguard genome...

Arabinosyl (ara) nucleoside analogues such as vidarabine (araA) and cytarabine (araC) are antimetabolites known to inhibit DNA polymerases in isolation. However, their effects on DNA synthesis catalys...

Cameron et al. show that dormant pre-replication complexes are removed upon collision with replication forks in Xenopus egg extracts. They identify FANCJ and RTEL1 as key factors in this process and s...

Cryogenic electron microscopy captures nucleotide excision repair complexes assembling on damaged DNA templates.

Replication stress induces the formation of transient chromatin loops that enclose de novo heterochromatin-enriched stalled replication forks.

Helicase-driven copy-back RNA synthesis in coronaviruses reveals how antivirals trap replication intermediates.

Here the authors show that collapse of single replication forks causes recombination, end fusions or degradation but does not restart DNA synthesis. In contrast, collapse of convergent forks completes...

Nature - PCNA–PAF15 has a key role in determining replisome dynamics during genome replication and protecting against genome instability.

Protein-protein interactions (PPIs) drive virtually all biological processes, yet most PPIs have not been identified and even more remain structurally unresolved. We developed a two-step computational...

Telomerase counteracts telomere shortening by repeatedly adding DNA repeats to chromosome ends. We identified the replication protein A (RPA) heterotrimer as a telomerase processivity factor critical ...

Abstract. Most bacterial sigma factors (σ) contain a highly conserved structural module, the ‘σ-finger’, which forms a loop that protrudes towards the RNA

Abstract. Disassembly of the replication machinery (replisome) from chromatin is an active process driven by two ubiquitin ligases Cul2LRR1 and TRAIP, whic

A multigenerational single-cell tracking approach provides a framework to dissect phenotypic plasticity at the single-cell level, offering insights into cellular processes that may resemble early even...

A head-to-head double-ring complex of the human multifunctional DNA repair protein RAD52 mediates protection of stalled replication forks during replication stress, protecting them from reversal by SM...

How genomic DNA is folded during cell division to form the characteristic rod-shaped mitotic chromosomes essential for faithful genome inheritance is …

Replication fork collapse at single-strand DNA breaks (SSBs) poses a serious threat to genome stability. Using Xenopus egg extracts, we show that a replication fork encountering an SSB on either the l...

DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...

SCF–FBXO31 scans proteins for C-terminal amidation and marks them for subsequent proteasomal degradation.

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Cacioppo, Gillis, Shlamovitz, Zeller, et al. show that CRL3ARMC5 ubiquitylates RNA Pol II at early stages of transcription as part of a major homeostatic RNA Pol II turnover mechanism. CRL3ARMC5 and the Integrator phosphatase act as complementary pathways, monitoring the quantity and quality of RNA Pol II complexes before they are licensed into elongation.

Nature - Biochemical and structural studies of the loading pathway of the human MCM2–7 replicative helicase motor reveal differences from the orthologous yeast system that may increase...