Some proteins mimic the repressive mark H3K27me3, but the physiological relevance of this phenomenon was unclear. Agius et al. show that the PRC2 subunits JARID2 and PALI1 mimic H3K27me3 to antagonize PRC2-mediated H3K27me3 deposition, limit Polycomb domain spreading, and enable timely activation of developmental genes in vivo.

RNA polymerase II (Pol II) abundance and transcriptional output are coordinated across diverse conditions to maintain mRNA homeostasis. To determine whether Pol II acts as a limiting factor for transc...

DNA is scattered with obstacles that stall RNA polymerase II (Pol II) and block the production of full-length transcripts. Two mechanisms are known to resolve stalled Pol II: transcription-coupled nuc...

Optimised nanobody-based quenchbodies are developed through rational design and in vitro evolution, enabling improved protein detection with up to 2.4-fold fluorescence increase and 2 nM sensitivity f...

The immune system is a spatially distributed, whole-body sensory-reactive system that monitors, reacts, and adapts to various stress signals (e.g., tissue

Nature Communications - Gunawan et al. propose an Extensible Immunofluorescence (ExIF) strategy that integrates distinct 4-plex image panels from routine fluorescence microscopy into multiplexed...

Research Assistant / Research Associate (Fixed Term) in the Department of Plant Sciences at the University of Cambridge.

Cacioppo, Gillis, Shlamovitz, Zeller, et al. show that CRL3ARMC5 ubiquitylates RNA Pol II at early stages of transcription as part of a major homeostatic RNA Pol II turnover mechanism. CRL3ARMC5 and the Integrator phosphatase act as complementary pathways, monitoring the quantity and quality of RNA Pol II complexes before they are licensed into elongation.

Kelley et al. discover that the PNUTS phosphatase complex plays an essential role in gene transcription by controlling RNA polymerase II pause release. PNUTS achieves this through its TND, which recog...