Brogan and Rudner report the identification of a microbial autoproteolytic domain that is structurally homologous to the GAIN domain of eukaryotic adhesion GPCRs. Rather than participating in transmem...

DNA replication depends on loading Cdc45 onto the MCM2-7 helicase to start DNA unwinding. Here, the authors use cryo-EM to show how phosphorylation enables Sld3-Sld7 to bind MCM2-7 and position Cdc45 ...

The cell wall peptidoglycan (PG) protects virtually all bacteria from osmotic lysis and specifies cell shape. Synthesis of this exoskeleton is carr...

Folddisco enables protein structural motif search in million scale databases.

This work introduces the Runs N’ Poses dataset for benchmarking deep learning methods on the protein–ligand complex prediction task. It shows that current methods rely on memorization, challenging the...

How good is Bacillus subtilis as a model for the spore-forming pathogen Bacillus anthracis? Using high throughput genetics to identify B. anthracis sporulation genes and cytological analysis of the mu...

Intragenomic conflict with selfish genetic elements spurs adaptive changes in subunits of essential multiprotein complexes. Whether and how these adaptive changes disrupt interactions within such comp...

Pilley et al. describe how metabolite levels change in 12 organs from male and female mice across five ages, spanning adolescence to old age. They reveal that organs show distinct age- and sex-specific changes in metabolism. They also develop metabolic clocks that predict putative metabolic drivers of aging, including hydroxyproline.

We invite applicants for a postdoctoral fellow position in the Brown lab at Harvard Medical School in Boston, Massachusetts. The Brown lab uses structural, biophysical, and biochemical approaches to d...

Protein-protein interactions (PPIs) drive virtually all biological processes, yet most PPIs have not been identified and even more remain structurally unresolved. We developed a two-step computational...

YouTube video by MRC Laboratory of Molecular Biology

A free interactive resource for viewing predicted AlphaFold multimer structures for protein pairs in biological pathways.

Brogan et al. uncover a signalling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory pathway decreases turgor ...

A study presents EndoMAP.v1, a resource that combines information on protein interactions and crosslink-supported structural predictions to map the interaction landscape of early endosomes.

Study solves riddle of how mutations in Integrator complex relate to neurodegeneration, cancer

Computational design of molecular recognition remains challenging despite advances in deep learning. The design of proteins that bind to small molecules has been particularly difficult because it requ...

ARMC1 partitions between distinct outer mitochondrial membrane complexes and the cytosol to control mitochondrial distribution.

A multi-protein system called the divisome promotes bacterial division. This apparatus synthesizes the peptidoglycan (PG) cell wall layer that forms the daughter cell poles and protects them from osmo...

Schmid and Walter train a classifier that discerns functionally relevant structure predictions in proteome-wide protein-protein interaction (PPI) screens using AlphaFold-Multimer, and they use this co...

During transcription, RNA polymerase II traverses through chromatin, and posttranslational modifications including histone methylations mark regions of active transcription. Histone protein H3 lysine ...