Anoxic live-cell imaging shows actin-dependent crawling motility and dynamic protrusions in Asgard archaea, providing insights into cellular innovations that may have contributed to eukaryotic evoluti...

Dynamic relocalization of MinD in Bacillus subtilis arises from membrane binding of monomers and dimers with membrane-triggered ATP hydrolysis, suggesting MinE-like activators are unnecessary for Min ...

Dynamic relocalization of MinD in Bacillus subtilis arises from membrane binding of monomers and dimers with membrane-triggered ATP hydrolysis, suggesting MinE-like activators are unnecessary for Min ...

Conformational biasing (CB) is a rapid and streamlined computational method that uses contrastive scoring by inverse folding models to predict protein variants biased toward desired conformational sta...

Nucleotide triphosphate (NTP)-dependent molecular switches regulate essential cellular processes by cycling between active and inactive states thro...

Borgs are large extrachromosomal elements of anaerobic methane-oxidizing archaea. Here, via in silico protein structure prediction of ~10,000 Borg proteins, the authors reveal that Borgs share numerou...

An exploration of the viromes of haloarchaea and their ultra-small DPANN symbionts reveals plasmid-derived satellites of viruses from both archaeal groups, highlighting the complexity of nested symbio...

BindCraft, an open-source, automated pipeline for de novo protein binder design with experimental success rates of 10–100%, leverages AlphaFold2 weights to generate binders with nanomolar affinity without the need for high-throughput screening.

ParABS systems partition chromosomal DNA and low-copy plasmids in bacteria. Here, the authors elucidate the mode of interaction between ParA and ParB and clarify how ParB stimulates the ATPase activity of ParA to drive the segregation process.

Bona fide gene clusters for archaella are widespread across a bacterial phylum and encode functional motility machinery.

Proteins SegA and SegB are important for chromosome segregation and organization in archaea of the order Sulfolobales, but mechanisms are unclear. Here, Kabli et al. uncover patterns and mechanisms th...

Self-labeling protein tags (SLPs) have revolutionized bioimaging by enabling precise protein tracking. A next-generation SLP, SNAP-tag2, has now been developed with improved labeling kinetics, fluores...

Bacteria, despite their diversity, use conserved cytoskeletal systems for their intracellular organization. In unicellular bacteria, the ParMRC DNA partitioning apparatus is well known for forming act...

It is unclear how cell compartmentalization emerged in prebiotic conditions. Now it is shown that a temperature gradient in a confined space can bring the core components of a cell together.

Histones are fundamental chromatin-organizing proteins in eukaryotes and archaea, where they assemble into (hyper)nucleosomes that wrap DNA. Recent studies have expanded the known repertoire of histon...

Updated phylogenetic analyses yield a more nuanced view of the evolution of the DPANN archaea, a diverse superphylum of enigmatic and symbiotic microorganisms with a debated placement on the tree of l...

Cell division in many archaea requires the coordinated activities of two distinct FtsZ proteins, which are part of the midcell division ring. Here, Liao et al. show that an additional protein, CdpA, o...

Ancestral reconstruction together with molecular dating of the last Asgard archaea and eukaryote common ancestor suggest that eukaryotes arose from the fusion of a H2-consuming archaeal host and a H2-...

42.1K Views. University of Groningen. This protocol provides a step-by-step procedure to monitor single cell behavior of different bacteria in time using automated fluorescence time-lapse microscopy....

The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes, rarely in bacteria, and only once in archaea. We show that uniaxial compression induces clonal mul...

A microbe from the Dead Sea switches to a tissue-like form when compressed