Dynamic relocalization of MinD in Bacillus subtilis arises from membrane binding of monomers and dimers with membrane-triggered ATP hydrolysis, suggesting MinE-like activators are unnecessary for Min ...

SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Alarmone synthesis depletes GTP levels leading to a GTP-dependent switch that controls triggered, spontaneous and antibiotic-induced persister formation in Bacillus subtilis.

42.1K Views. University of Groningen. This protocol provides a step-by-step procedure to monitor single cell behavior of different bacteria in time using automated fluorescence time-lapse microscopy....