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Gabriel Rocklin

@grocklin.bsky.social
1.5K followers 389 following 164 posts

Digests proteins. High-throughput protein biophysics and design, Northwestern University. www.rocklinlab.org

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Reposted by Gabriel Rocklin
Yehlin Cho @yehlincho.bsky.social · 21/05/2026
🚀 Excited to share our new work: Absolute Stability Predictor! 📊: forms.gle/4ZnXZSnTBvay... Built the MGnify Stability Dataset (1.8M+ measurements) and developed stability prediction models, together with @grocklin.bsky.social @KotaroTsuboyama, @sokrypton.org and teams.
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Gabriel Rocklin @grocklin.bsky.social · 21/05/2026
We've actually been working on this very actively and have quite promising results on larger/higher stability proteins using an improved method. Looking for funding to buy some (a lot of) oligos... any pointers? It's within reach...
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Kresten Lindorff-Larsen @lindorfflarsen.bsky.social · 21/05/2026
Another banger
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Gabriel Rocklin @grocklin.bsky.social · 21/05/2026
Thread coming later but I think people will like this!! 2 million stability measurements plus a predictive model for deltaG (not ddG)
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bioRxiv Biophysics @biorxiv-biophys.bsky.social · 21/05/2026
Accurate protein stability prediction for small domains using mega-scale experiments www.biorxiv.org/content/10.64898/20…
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Gabriel Rocklin @grocklin.bsky.social · 18/05/2026
Happy to share this in its published form! More than any project I've worked on, this was a huge team effort, so thank you so much to that team! Especially @ajrferrari.bsky.social www.nature.com/articles/s41...
nature.com
Large-scale discovery, analysis and design of protein energy landscapes - Nature
An analysis of 5,778 domains 28–64 amino acids in length reveals hidden variation in conformational fluctuations, even between sequences sharing the same fold and global folding stability.
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Kresten Lindorff-Larsen @lindorfflarsen.bsky.social · 13/05/2026
If you missed the preprint, now is a good time to read the journal version of @grocklin.bsky.social et al’s fantastic paper on multiplexed HDX measurements Large-scale discovery, analysis and design of protein energy landscapes doi.org/10.1038/s415...
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Alondra Nelson @alondra.bsky.social · 18/04/2026
Another example demonstrating that the federal science and research ecosystem is being seriously damaged regardless of what’s ultimately appropriated
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I-75 Scientist @flscitriguy.bsky.social · 19/03/2026
NIGMS is signed onto the F31 NOFO. grants.nih.gov/grants/guide...
grants.nih.gov
NOT-GM-26-002: Notice of NIGMS Participation in PA-25-422 "Ruth L. Kirschstein National Research Service Award (NRSA) Individual Predoctoral Fellowship (Parent F31)"
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Notice of NIGMS Participation in PA-25-422
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Calin Plesa @calin.bsky.social · 03/03/2026
New lab preprint: if ML struggles with extrapolation, let's expand the diversity of training data with gene synth, DNA shuffling, and ML gen.... also lots of #FluorescentProteins #ProteinEngineering #MachineLearning #SyntheticBiology 1/n
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bioRxiv Synthetic Biology @biorxiv-synthbio.bsky.social · 03/03/2026
High Diversity Gene Libraries Facilitate Machine Learning Guided Exploration of Fluorescent Protein Sequence Space www.biorxiv.org/content/10.64898/20…
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Nick Polizzi @nickpolizzi.bsky.social · 20/02/2026
I’m looking to hire a research technician for my lab at Harvard & DFCI, who would primarily work in the wet lab expressing and characterizing designed proteins, starting this summer. A great role for a recent college grad looking for an immersive research experience before grad school.
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Keating Lab @keatinglab.bsky.social · 18/01/2026
Here’s a preprint from the Keating Lab: www.biorxiv.org/content/10.6.... Foster Birnbaum and Amy E. Keating demonstrate that sequence design models, such as ProteinMPNN, are limited because they were trained only on native sequences.
biorxiv.org
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Michael Li (李之樸) @mcpli.bsky.social · 31/12/2025
The final Calvin and Hobbes, which appeared in papers 30 years ago today.
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AI x Bio Discovery @aixbiobot.bsky.social · 29/12/2025
Large scale prospective evaluation of co-folding across 557 Mac1-ligand complexes and three virtual screens [new] Mac1 co-folding eval: Pose prediction, conform. change, & hit ID post-training.
Large scale prospective evaluation of co-folding across 557 Mac1-ligand complexes and three virtual screensFigure 1Figure 2Figure 3
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Katie Mack @astrokatie.com · 30/11/2025
The most precious commodity you have is your attention. You don’t have to waste it on poor-faith debates or arguments with strangers if you don’t think they’ll be productive. You can prioritize the things that matter to you and make your life richer.
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Bryan Bryson @thebrysonlab.bsky.social · 05/11/2025
Excited to share our newest manuscript on antigen discovery and vaccine design for tuberculosis lead by Owen Leddy (everyone hiring new faculty in a few years, remember this name!) www.science.org/doi/10.1126/... #TBsky
science.org
Immunopeptidomics can inform the design of mRNA vaccines for the delivery of Mycobacterium tuberculosis MHC class II antigens
MHC class II immunopeptidomics in human phagocytes infected with Mycobacterium tuberculosis enables vaccine immunogen design and optimization.
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Gabriel Rocklin @grocklin.bsky.social · 19/11/2025
New preprint! We measured temperature- and pH-induced aggregation for over 18,000 natural and de novo designed protein domains!
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bioRxiv Biophysics @biorxiv-biophys.bsky.social · 13/11/2025
Global Analysis of Aggregation Determinants in Small Protein Domains www.biorxiv.org/content/10.1101/202…
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Boston Protein Design and Modeling Club @bpdmc.org · 22/10/2025
quick reminder to come see @grocklin.bsky.social tonight at @mit.edu!
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Boston Protein Design and Modeling Club @bpdmc.org · 17/10/2025
Join us in Room 181, Building 68 @mit.edu on October 22nd 2025 @ 7pm EDT to see @grocklin.bsky.social present a mind blowing amount of data and learn what becomes possible at this scale "Predicting protein folding stability and aggregation propensity using large-scale experiments" bpdmc.org
bpdmc.org
schedule
introduction and membership Boston Protein Design and Modeling Club (BPDMC) is a community of computational protein engineers and modelers from both academia and industry. While we are based in Boston...
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Erica D. Pratt @pratted.bsky.social · 26/09/2025
Announcing such a major change to NSF GRFP this late in the cycle is incredibly cruel. 2nd yr students (mine included) have been working so hard on their proposals despite ongoing uncertainty. They are driven and passionate about being outstanding scientists and helping those coming up behind them.
This means individuals in the following statuses at the time of application are eligible:

    Undergraduate in the final (senior) year of a bachelor’s degree program
    Bachelor’s degree-holder with NO enrollment in a graduate degree program (non-degree graduate coursework allowed)
    Individual enrolled in a joint bachelor’s-master’s degree program with at least three undergraduate years completed  
    First-year graduate student in their first graduate degree program with less than one academic year completed in the degree program (according to institution’s academic calendar)
        Individuals enrolled in joint bachelor’s-master’s degree programs are considered graduate students. For GRFP, joint bachelor’s-master’s degrees are defined as degrees concurrently pursued and awarded.
        Not be a current NSF employee.

Applications that do not meet eligibility requirements will be returned without review as being ineligible for a fellowship.
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Elise Cutts @elisecutts.bsky.social · 24/09/2025
Cecilia Payne-Gaposchkin ✨ figured out what stars are made of ✨ when she was just 25. 🔭🧪 Her PhD thesis basically established the Harvard astro department — at a time when Harvard didn't officially allow woman students. I wrote this little profile to mark the 100th anniversary of her thesis:
sciencenews.org
How a Harvard maverick forever changed our concept of the stars
At just 25, Cecilia Payne-Gaposchkin applied quantum physics to a treasure trove of astronomical observations to show that stars are mostly hydrogen and helium.
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Colette Delawalla, PhD @cdelawalla.bsky.social · 30/07/2025
Vought and his “no more NIH money out the door” footnote is a power struggle. Between R’s getting antsy about hard votes and Senate signaling support for science funding, Vought had to signal his dominance in some way. Now is the real test: are R’s more afraid of Trump et al. or their constituents?
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Max Kozlov @maxkozlov.bsky.social · 30/07/2025
The NIH can’t award ANY grants to outside researchers under new WH restriction, reports @wsj.com. The pause came in the form of a footnote from OMB Director Vought, in a document that doles out federal funds to the NIH. Prelude to rescissions, especially after his comments over the weekend?
wsj.com
Trump Administration Puts New Chokehold on Billions in Health-Research Funding
The National Institutes of Health can’t award grants to outside researchers under a new White House restriction.
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Gen Wojcik @genandgenes.bsky.social · 30/07/2025
One might think, oh the fiscal year is only two months. But many of our grants are already delayed by weeks to months and we are on borrowed time as is. This is devastating and heralds darker times ahead.
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Roel Verhaak @roelverhaak.bsky.social · 23/07/2025
PO just confirmed that our 5th percentile R01 is not getting funded. Past NCI Payline at 4 percent. Cant believe it.
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@LaBonneLaB @labonnelab.bsky.social · 24/07/2025
NCI's payline will be the 4th percentile. The Trump administration has effectively killed Cancer Research in the US www.cancer.gov/grants-train...
cancer.gov
Funding Policy - Research Grants
NCI funding policy for research project grant (RPG) awards reflects the funding goals of the institute, NIH, and HHS.
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Chris Ashwood @cashwood.proteaglyco.com · 16/07/2025
A motivating quote from @carolynbertozzi.bskyverified.social: “…You are discovering knowledge and gifting that to humanity for all of posterity. Once you learn something no one can take that away. It is not an object. It is an intellectual currency that will be shared throughout the generations…”
A personal message from 2022 chemistry laureate Carolyn Bertozzi to all young researchers on why to aspire to a career in science: 

”I would want to share with them how a life in science is incredibly rewarding. It is rewarding because it is creative. You are discovering knowledge and gifting that to humanity for all of posterity. Once you learn something no one can take that away. It is not an object. It is an intellectual currency that will be shared throughout the generations. And you created that. It has a permanence that ironically a physical object doesn’t have. You can paint a painting, but eventually it will fade. But knowledge doesn’t fade. Knowledge stays with us forever and it becomes the foundation for the next generations.”
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Brittany S. Morgan @morganlabatnd.bsky.social · 03/07/2025
#MorganLabatND is hiring a research technician!! We are looking for an aspiring biochemistry researcher that shares our community values, is curious about the world around them, and brings a unique perspective to our team. (1/2) jobs.smartrecruiters.com/UniversityOf...
jobs.smartrecruiters.com
Research Technician
Company Description: The University of Notre Dame is more than a workplace; it is a vibrant, mission-driven community where every employee is valued and supported. Rooted in a tradition of excellence ...
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Gabriel Rocklin @grocklin.bsky.social · 03/07/2025
Chicago!
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Science for Good ☀️ @sciforgood.bsky.social · 26/06/2025
Although 500 new NSF GRFPs were added in June, the total number of fellowships in 2025 remains ~500 fewer than in 2024. Some fields—like CS and Physics—still saw net increases in total awards. But others, including Life Sciences & Psychology, have taken hits of 50% or more compared to last year.
Change in NSF GRFP awards from 2024-2025. Bar height shows absolute change, label shows percent change.
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Daniel Arango @danyarango.bsky.social · 18/06/2025
Excited to share this preprint from my lab. We report a potent and site-specific inhibition of translation initiation by 2’O methylation. It’s been a though year, but these discoveries keep us excited and motivated! #RNAsky #RNAbiology
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Ben Orr @benorr.bsky.social · 10/06/2025
We are excited to share our new preprint, “An improved model for prediction of de novo designed proteins with diverse geometries”. (1/9) www.biorxiv.org/content/10.1...
biorxiv.org
An improved model for prediction of de novo designed proteins with diverse geometries
Nature uses structural variations on protein folds to fine-tune the geometries of proteins for diverse functions, yet deep learning-based de novo protein design methods generate highly regular, ideali...
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Needhi Bhalla 💅🏽 @needhibhalla.bsky.social · 07/06/2025
Keep in mind: these are AWARDED grants, with Notice of Awards. The federal government is not honoring existing contracts with this university, refusing to reimburse for costs charge on awarded grants.
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Jeremy Berg @jeremymberg.bsky.social · 07/06/2025
Funding freeze at Northwestern from Emma Mairson at grant-watch.us. Data from USAspending. Normally, ~$70 M per month. Now, bupkis. Northwestern is keeping research moving and must be spending ~$40 M per month. Clearly not sustainable for too long.
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Craig Kaplan @triggerloop.bsky.social · 04/06/2025
Note that this (exceptional) visualization does not appear to account for the Harvard, Brown, Northwestern terminations. This is a major underestimate
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Patricia L. Clark @ndbiochemist.bsky.social · 02/06/2025
Do you love flow cytometry, cell sorting and other quantitative, biophysical approaches for measuring cells and their biomolecules? Join our team at Notre Dame! www.linkedin.com/posts/ndbic_...
linkedin.com
Postdoctoral Research Associate | ND Biophysics Instrumentation Core Facility
Join our team at the ND Biophysics Instrumentation Core Facility! We're seeking a Postdoctoral Scholar to work at the forefront of optical and other techniques to measure biomolecular structure an...
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lizkellogg.bsky.social @lizkellogg.bsky.social · 02/06/2025
our lab is blessed to have 3 new postdoctoral fellow positions. we would welcome scientists interested in...
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Josh Zingher @zingherpolisci.bsky.social · 28/05/2025
The notion that private institutions need to toe the party line is so fundamentally un-American it is almost unbelievable
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Michael Baym @baym.lol · 24/05/2025
I appreciate the offers from other scientists to help Harvard (students, labs, etc) through this attack, but what would be most helpful is for you to use your position to contact your elected representatives and make it clear this fight isn't just Harvard, it will affect you, and their districts too
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Eric Topol @erictopol.bsky.social · 22/05/2025
Good cover @economist.com
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The Ferrari Lab @theferrarilab.bsky.social · 23/05/2025
The Ivy League should be preparing mutual defense agreements that would allow students to transfer, even just nominally, to any other Ivy. Then the Big10 should do the same. We succeed together, we fail apart.
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Jameel Jaffer @jameeljaffer.bsky.social · 23/05/2025
This is especially important: “We call upon our fellow institutions of higher education in the United States to declare their willingness to facilitate the immediate co-enrollment of Harvard’s international students, including formal acceptance of transfer for immigration purposes.”
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Dani Rodrik @drodrik.bsky.social · 23/05/2025
Three things made the US a rich and powerful nation: the rule of law, its science & innovation system, and openness to foreign talent. Remarkable how Trump has taken a sledgehammer to all three. No enemy of this country could do more.
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Michael Baym @baym.lol · 14/05/2025
Yesterday, the NIH R35 “Outstanding Investigator” grant to fund scientists in my lab studying antibiotic resistance was terminated for reasons not related to the content of the science, or any actions taken by me or members of my lab
A screenshot of the termination notice showing "Outstanding Investigator Grants"A screenshot of the termination notice with "This award is terminated effective the date of this award, due to unsafe antisemitic actions that suggest the institution lacks concern for the safety and wellbeing of Jewish students." highlighted
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Gabriel Rocklin @grocklin.bsky.social · 09/05/2025
youtu.be/8MaWf2B-Th0?...
youtu.be
Dvořák: String Quartet No. 14 in A Flat Major, Op. 105, B. 193 - II. Molto vivace
YouTube video by Prague String Quintet - Topic
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Jeremy Berg @jeremymberg.bsky.social · 21/04/2025
“However, the NIH General Counsel’s office was very clear that this was not possible. The statutory authority for NIH is to support research and NIH does not have a say in how this is accomplished (as long as it is legal).” /fin
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@LaBonneLaB @labonnelab.bsky.social · 21/04/2025
Should say "How the federal government became so deppendent on academic research labs"
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Jeremy Berg @jeremymberg.bsky.social · 18/04/2025
Sources have shared 2 important pieces of information about NIH grant payments (although I am trying to get further confirmation). Hang on... (1) ALL payments to Columbia, Brown, Northwestern, Cornell, Weill-Cornell, Harvard are frozen. 1/3
media.tenor.com
a close up of a red light that looks like a heart on make a gif .
ALT: a close up of a red light that looks like a heart on make a gif .
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