Biochemical, structural and cell biological analyses reveal that UAP56 (DDX39B) assembles with a TREX-2–like module that redirects non-functional polyadenylated RNAs from export to degradati...

Execution of mRNA cleavage in nonsense-mediated decay remained elusive. The authors show that SMG5 complements SMG6 to form a highly active, composite endonuclease with expanded catalytic center that ...

Translation inhibitors are invaluable for probing ribosome function and therapeutic applications, but systematic discovery in human systems is limited by the lack of scalable, screening-compatible cel...

UPF1 depletion inhibits nonsense-mediated mRNA decay (NMD) and stabilizes direct target transcripts within hours. Boehm et al. use conditional protein degradation and advanced transcriptomics to map t...

Cell-free translation is a powerful tool for studying protein synthesis. Here, we present a protocol for monitoring mRNA translation and degradation u…

Vacancy at Department of Molecular Biology and Genetics - RNA Biology and Innovation, Aarhus University

m6A is a potent inducer of ribosome stalling and collisions that trigger YTHDF-mediated mRNA degradation, a process that highlights the ribosome as a critical sensor linking translation dynamics to m6...

Intracellular pathogens hijack host gene expression to subvert cellular processes. Here, the authors show that Legionella pneumophila’s PieF effector inhibits the human CCR4-NOT deadenylation machiner...

The heterogeneity of isoform level m6A RNA methylation in single cells is unclear. The authors characterize m6A at both single-cell and isoform level through ONT long-read sequencing on single-cell cD...

Sharma, Jiao and colleagues report glycosylation of small RNAs within exosomes and propose a glycosylation-dependent mechanism for RNA targeting into exosomes, which may influence intercellular commun...

Cell-free translation systems are indispensable for studying protein synthesis, enabling researchers to explore translational regulation across different cell types. The difficulties in producing cell...

The noncanonical translation initiation factor eIF2A plays critical roles in diverse cellular processes, including the integrated stress response, neurodegeneration and tumorigenesis. However, the pre...

Chemically modified nucleotides in mRNA are critical regulators of gene expression, primarily through interactions with reader proteins that bind to t…

Using high-throughput functional profiling, how disordered regions control mRNA stability and translation is explored.

Bicknell et al. demonstrate that exogenously delivered mRNAs are degraded in a translation-dependent manner at a rate that correlates with ribosome load. ORF sequences leading to high ribosome load ca...

Upon intramuscular administration, COVID-19 mRNA vaccines are primarily taken up by macrophages, in which the cellular machinery extends their poly(A) tails, thereby increasing mRNA stability and tran...

Soles et al. demonstrate that the combination of a 5′ splice site and a poly(A) junction constitutes a nuclear RNA degradation code that targets RNAs for degradation by the PAXT adaptor and the RNA ex...

The ATP-dependent RNA helicase Up-frameshift 1 (UPF1) is an essential protein in mammalian cells and a key factor in nonsense-mediated mRNA decay (NMD), a translation-dependent mRNA surveillance proce...

Bäumlin et al. investigate the roles of coronavirus Nsp1 in translation inhibition and mRNA degradation using a human cell-free translation system. They demonstrate that SARS-CoV-2 Nsp1 mediates mRNA ...

Mammalian cells present RNA-binding proteins on the cell surface that form clustered domains containing glycoRNAs.

YouTube video by NCCR RNA & Disease

Using a deep neural network and statistical analyses of atomic force microscopy images of individual RNA molecules enables the mapping of RNA conformational space in solution.

Antiviral signaling downstream of RIG-I–like receptors (RLRs) proceeds through a multi-protein complex organized around the adaptor protein mitochondrial antiviral signaling protein (MAVS). Protein co...

Cell-free translation systems are indispensable for studying protein synthesis, enabling researchers to explore translational regulation across different cell types. Here, we present a scalable method...

Allosteric communication between the eIF4F subunits drives mRNA cap-recognition and subsequent activation of the message.

A compound that enhances homology-directed repair in CRISPR editing leads to genome instability.

Myriad families of natural RNAs have been proposed, but not yet experimentally shown, to form biologically important structures. Here we report three-dimensional structures of three large ornate bacte...

Translation entails ribosomes reading a gene’s messenger RNA transcript to generate a protein. Image credit: ART-ur/ Shutterstock.

Kamel et al. apply complementary approaches to elucidate the composition of Sindbis virus ribonucleoproteins. They identified a subset of nuclear proteins that selectively translocate to the cytoplasm, interact with viral RNA, and suppress virus infection. Among them, U2 snRNP emerges as a ribonucleoprotein complex with moonlighting antiviral activity.

The CCR4-NOT complex is a major regulator of eukaryotic messenger RNA (mRNA) stability. Slow decoding during translation promotes association of CCR4-NOT with ribosomes, accelerating mRNA degradation....

Zhou et al. discovered a specific role of adenosine modifications in the coding region of mRNAs. These chemical alterations slow down the movement of the ribosome during translation and trigger degrad...

imageimageTranscriptome-wide measurement of subcellular mRNA dynamics in mouse ES cells reveals that nuclear retention is the rate-limiting step in the life-cycle of most mRNAs and provides a resource for understanding spatiotemporal gene ...