The kinase ZAK is activated at collided ribosomes to mediate the ribotoxic stress response.

Translation inhibitors are invaluable for probing ribosome function and therapeutic applications, but systematic discovery in human systems is limited by the lack of scalable, screening-compatible cel...

Gade et al. report that polysomes and mRNA levels impact the biophysical properties of the cytoplasm. Using passive rheology probes and single-particle tracking, they demonstrate that intracellular diffusion is enhanced when polysomes are disassembled or when mRNA levels are reduced in eukaryotic cells.

Cell-free translation is a powerful tool for studying protein synthesis. Here, we present a protocol for monitoring mRNA translation and degradation u…

Nature Biotechnology - A deep convolutional neural network model predicts the influence of the full-length mRNA sequence on translation efficiency.

Matched ribosome profiling and RNA sequencing data quantify translation efficiency patterns across 140 human and mouse cell lines.

Intracellular pathogens hijack host gene expression to subvert cellular processes. Here, the authors show that Legionella pneumophila’s PieF effector inhibits the human CCR4-NOT deadenylation machiner...

Helicobacter pylori CagA protein strongly inhibits functional and pathogenic amyloid fibril formation in both bacteria and humans.

Bäumlin et al. investigate the roles of coronavirus Nsp1 in translation inhibition and mRNA degradation using a human cell-free translation system. They demonstrate that SARS-CoV-2 Nsp1 mediates mRNA degradation independently of translation, whereas MERS-CoV Nsp1 inhibits translation without degradation, revealing distinct mechanisms and co-evolutionary protection of viral mRNAs.

Bäumlin et al. investigate the roles of coronavirus Nsp1 in translation inhibition and mRNA degradation using a human cell-free translation system. They demonstrate that SARS-CoV-2 Nsp1 mediates mRNA ...

Certain viruses with bicistronic mRNAs use reinitiation to translate the downstream open reading frame. Sherlock et al. use bioinformatics to find new examples of RNA structures that mediate reinitiat...

RNA plays a central role in protein biosynthesis and performs diverse regulatory and catalytic functions, making it essential for all processes of life. Like DNA, RNA is constantly subjected to damage...

There are many large language models to choose from; some excel at coding, whereas others are better for synthesizing information.

Certain viruses with bicistronic mRNAs use reinitiation to translate the downstream open reading frame. Sherlock et al. use bioinformatics to find new examples of RNA structures that mediate reinitiat...

Nature Reviews Molecular Cell Biology, Published online: 24 January 2025; doi:10.1038/s41580-024-00818-9Many new small RNA species and their derived fragments have been recently identified in animals. This Review discusses the biogenesis and functions of…

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Large-scale, structure-guided computational pipeline sifts through millions of phage proteins to identify those that bind and antagonize host immune proteins. Detected proteins are shown to inhibit no...

Recent sequencing-based methods, including novel ribosome profiling, ribosome affinity purification and spatial translation methods, have enabled the measurement of translation at single-cell resoluti...

Large-scale experimental analysis of Human Domainome 1, a library containing more than 500,000 missense mutation variants across more than 500 human protein domains, reveals that 60% of pathogenic mis...